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On-line monitoring of the denitrification process by measurement of NADH fluorescence

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Abstract

On-line monitoring method of the denitrification process was developed by NADH fluorescence measurements using the facultative microorganism, Ochrobactrum anthropi SY509. The NADH fluorescence signals showed a rapid drop and a rise at the initiation and termination points of the denitrification, respectively. This NADH fluorescence method could be applied successfully to the monitoring of the denitrification process in sequential batch and continuous operations while these rapid changes of fluorescence were not observed in a batch operation due to accumulation of some metabolites secreted from the microorganism.

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Authors and Affiliations

  1. School of Chemical Engineering, Seoul National University, Seoul, 151-742, Republic of Korea

    Dohoon Lee & Young Je Yoo

  2. Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University, Seoul, 151-742, Republic of Korea

    Seung Hoon Song

  3. Biotechnology and Chemicals Industries Division, Ministry of Commerce, Industry and Energy, Kyonggi-Do, 427-723, Republic of Korea

    Ji Hyeon Kim

Authors
  1. Dohoon Lee
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  2. Seung Hoon Song
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  3. Ji Hyeon Kim
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  4. Young Je Yoo
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Lee, D., Hoon Song, S., Kim, J.H. et al. On-line monitoring of the denitrification process by measurement of NADH fluorescence. Biotechnology Letters 24, 949–952 (2002). https://doi.org/10.1023/A:1015577132408

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  • DOI: https://doi.org/10.1023/A:1015577132408

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