Abstract
The kinetics of the α-chymotrypsin catalysed transesterification of N-acetyl-l-phenylalanine ethyl ester with 1-butanol and the competing hydrolysis were evaluated at fixed water activity in two ionic liquids and two non-ionic organic solvents. In most respects the four solvents behaved similarly. However, at a water activity of 0.33, higher catalytic activity was observed in the ionic liquid, 1-butyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]amide, than in the other solvents, and at aw=0.11 catalysis was only observed in this solvent.
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Eckstein, M., Sesing, M., Kragl, U. et al. At low water activity α-chymotrypsin is more active in an ionic liquid than in non-ionic organic solvents. Biotechnology Letters 24, 867–872 (2002). https://doi.org/10.1023/A:1015564608261
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DOI: https://doi.org/10.1023/A:1015564608261