Abstract
Sex differences in the kinetic parameters of p-aminohippuric acid (PAH) transport in brush border (BBMV) and basolateral (BLMV) membrane vesicles from kidney cortex have been observed. Membrane fluidity of BBMV was higher in females as compared with male rats as indicated by anisotropy values (0.1897 ± 0.0010 vs. 0.2003 ± 0.0014, p < 0.05, for females and males respectively). Membrane fluidity of BLMV were similar in both sexes. Western blot studies revealed that OAT1 protein in female BLMV was present at only 40% of level found in BLMV from male rats. The lower expression of OAT1 in BLMV in association with the higher BBMV fluidity (which may affect the affinity of PAH transporter in this membrane domain) observed in females may be responsible, at least in part, for the gender difference described in renal PAH secretion.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brenner BB, Rector FC: In: The Kidney, 5th edn. W.B. Saunders Company, Pennsylvania, USA, 1996, 607–625
Burckhardt G, Wolff N: Structure of renal organic anion and cation transporters. Am J Physiol 278: F853–F866, 2000
Inui K-I, Masuda S, Saito H: Cellular and molecular aspects of drug transport in the kidney. Kidney Int 58: 944–958, 2000
Van Aubel R, Masereeuw R, Russel FGM: Molecular pharmacology of organic anion transporters. Am J Physiol 279: F216–F232, 2000
Berkhin EB, Humphreys MH: Regulation of renal tubular secretion of organic compounds. Kidney Int 59: 17–30, 2001
Sekine T, Watanabe N, Hosoyamada M, Kanai Y, Endou H: Expression cloning and characterization of a novel multispecific organic anion transporter. J Biol Chem 272: 18526–18529, 1997
Sweet DH, Wolff NA, Pritchard JB: Expression cloning and characterization of ROAT1: The basolateral organic anion transporter in rat kidney. J Biol Chem 272: 30088–30095, 1997
Van Aubel RAM, Peters JG, Masereeuw R, Van Os CH, Russel FGM: Multidrug resistance protein Mrp2 mediates ATP-dependent transport of classical renal organic anion p-aminohippurate. Am J Physiol 279: F713–F717, 2000
Leier I, Hummel-Eisenbeiss J, Cui Y, Keppler D: ATP-dependent paraaminohippurate transport by apical multidrug resistance protein MRP2. Kidney Int 57: 1636–1642, 2000
Bowman HM, Hook JB: Sex differences in organic ion transport by rat kidney. Proc Soc Exp Biol Med 141: 258–262, 1972
Braunlich H, Meinig T, Grosch U: Postnatal development of sex differences in renal tubular transport of p-aminohippurate (PAH) in rats. Exp Toxic Pathol 45: 309–313, 1993
Reyes JL, Melendez E, Alegria A, Jaramillo Juarez F: Influence of sex differences on the renal secretion of organic anions. Endocrinology 139: 1581–1587, 1998
Cerrutti JA, Quaglia NB, Torres AM: Characterization of the mechanisms involved in the gender differences in p-aminohippurate renal elimination in rats. Can J Physiol Pharmacol 79: 805–813, 2001
Simon FR, Fortune J, Iwahashi M, Bowman S, Wolkoff A, Sutherland E: Characterization of the mechanisms involved in the gender differences in hepatic taurocholate uptake. Am J Physiol 276: G556–G565, 1999
Montagna G, Hofer C, Torres AM: Impairment of cellular redox status and membrane proteins activities in kidneys from rats with ischemic acute renal failure. Biochim Biophys Acta 1407: 99–108, 1998
Jensen RE, Berndt WO: Epinephrine and norephinephrine enhance p-aminohippurate transport into basolateral membrane vesicles. J Pharmacol Exp Ther 244: 543–549, 1988
Schoner W, Von Ilberg C, Kramer R, Seubert W: On the mechanism of Na+ and K+ stimulation hydrolysis of adenosine triphosphate. Eur J Biochem 1: 334–343, 1967
Widnell CB: Purification of rat liver 5′ nucleotidase as a complex with sphingomyelin. Meth Enzymol 32: 368–374, 1974
Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–257, 1976
Torres AM, Lunazzi G, Stremmel W, Tiribelli C: Bilitranslocase and BSP/bilirubin binding protein are both involved in the hepatic uptake of organic anions. Proc Natl Acad Sci USA 90: 8136–8139, 1993
Shinitzky M, Barenholz Y: Dynamics of the hydrocarbon layer in liposomes of lecitin and sphingomyelin containing dicetylphosphate. J Biol Chem 249: 2652–2657, 1974
Shinitzky M: In: M. Shinitzky (ed). Physiology of Membrane Fluidity. CRC Press, Boca Raton, 1984, pp 1–52
Le Grimellec C, Friedlander G, Yandouzi EHE, Zlatkine P, Giocondi MC: Membrane fluidity and transport properties in epithelia. Kidney Int 42: 825–836, 1992
Urakami Y, Nakamura N, Takahashi K, Okuda M, Saito H, Hashimoto Y, Inui K-I: Gender differences in expression of organic cation transporter OCT2 in rat kidney. FEBS Lett 461: 339–342, 1999
Lu R, Kanai N, Bao Y, Wolkoff AW, Schuster VL: Regulation of renal oatp mRNA expression by testosterone. Am J Physiol 270: F332–F337, 1996
Urakami Y, Okuda M, Saito H, Hashimoto Y, Inui K-I: Hormonal regulation of organic cation transporter OCT2 expression in rat kidney. FEBS Lett 473: 173–176, 2000
Rights and permissions
About this article
Cite this article
Cerrutti, J.A., Brandoni, A., Quaglia, N.B. et al. Sex differences in p-aminohippuric acid transport in rat kidney: Role of membrane fluidity and expression of OAT1. Mol Cell Biochem 233, 175–179 (2002). https://doi.org/10.1023/A:1015563021602
Issue Date:
DOI: https://doi.org/10.1023/A:1015563021602