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Desulfurization of light gas oil by a novel recombinant strain from Pseudomonas aeruginosa

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Abstract

The dsz desulfurization gene cluster from Rhodococcus erythropolis KA2-5-1 was transferred into the chromosomes of Pseudomonas aeruginosa NCIMB 9571 by using a transposon vector. Resting cells of the recombinant strain, PAR41, desulfurized 63 mg sulfur l−1 of light gas oil (LGO) containing 360 mg S l−1. The desulfurization activity for LGO by the resting cells of strain PAR41 grown with n-tetradecane (50% v/v) was much higher (10∼18-fold) than in glucose-grown cells. P. aeruginosa NCIMB 9571 is able to take up water-insoluble compounds from an oil phase which is enhanced by n-alkane.

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References

  • Alexeyev MF, Shokolenko IN, Croughan TP (1995) New mini-Tn5 derivatives for insertion mutagenesis and genetic engineering in Gram-negative bacteria. Can. J. Microbiol. 41: 1053-1055.

    Google Scholar 

  • Beal R, Betts WB (2000) Role of rhamnolipid biosurfactants in the uptake and mineralization of hexadecane in Pseudomonas aeruginosa. J. Appl. Microbiol. 89; 158-168.

    Google Scholar 

  • Csordas-Toth E, Boros I, Venetianer P (1979) Structure of the promoter region for the rrnB gene in Escherichia coli. Nucl. Acids Res. 7: 2189-2197.

    Google Scholar 

  • Gallardo ME, Ferrandez A, Lorenzo AF, Garcia JL, Diaz E (1997) Designing recombinant Pseudomonas strains to enhance biodesulfurization. J. Bacteriol. 179: 7156-7160.

    Google Scholar 

  • Gray KA, Pogrebinsky OS, Mrachko GT, Montecello LXiDJ, Squires CS (1996) Molecular mechanisms of biocatalytic desulfurization of fossil fuels. Nat. Biotechnol. 14: 1705-1709.

    Google Scholar 

  • Hirasawa K, Ishii Y, Kobayashi M, Koizumi K, Maruhashi K (2001) Improvement of desulfurization activity in Rhodococcus erythropolis KA2-5-1 by genetic engineering. Biosci. Biotechnol. Biochem. 65: 239-246.

    Google Scholar 

  • Kabe T, Ishihara A, Tajima H (1992) Hydrodesulfurization of sulfur containing polyaromatic compounds in light oil. Ind. Eng. Chem. Res. 31: 1577-1580.

    Google Scholar 

  • Kobayashi M, Onaka T, Ishii Y, Konishi J, Takaki M, Okada H, Ohta Y, Koizumi K, Suzuki M (2000) Desulfurization of alkylated forms of both dibenzothiophene and benzothiophene by a single bacterial strain. FEMS Microbiol. Lett. 187: 123-126.

    Google Scholar 

  • Koch AK, Kappeli O, Fiechter A, Reiser J (1991) Hydrocarbon assimilation and biosurfactant production in Pseudomonas aeruginosa mutants. J. Bacteriol. 173: 4212-4219.

    Google Scholar 

  • Monticello DJ (2000) Biodesulfurization and the upgrading of petroleum distillates. Curr. Opin. Biotechnol. 11: 540-546.

    Google Scholar 

  • Monticello DJ, Finnerty ER (1985) Microbial desulfurization of fossil fuels. Ann. Rev. Microbiol. 39: 371-389.

    Google Scholar 

  • Onaka T, Okumura K, Suzuki M (1997) Analysis of biodesulfurization products using solid-phase extraction. J. Chromatogr. Sci. 35: 417-422.

    Google Scholar 

  • Setti L, Lanzarini G, Pafferi PG (1995) Dibenzothiophene biodegradation by a Pseudomonas sp. in model solutions. Process Biochem. 30: 721-728.

    Google Scholar 

  • Siegmund I, Wagner F (1991) New method for detecting rhamnolipids excreted by Pseudomonas species during growth on mineral agar. Biotechnol. Tech. 5: 265-268.

    Google Scholar 

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Watanabe, K., Noda, Ki., Ohta, Y. et al. Desulfurization of light gas oil by a novel recombinant strain from Pseudomonas aeruginosa . Biotechnology Letters 24, 897–903 (2002). https://doi.org/10.1023/A:1015549600509

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  • DOI: https://doi.org/10.1023/A:1015549600509

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