Abstract
Detection and identification of members of the genus Streptomyces are of great value because they provide a rich source of antibiotics. Toward the goal of identifying additional novel antibiotics, a total of 292 different Streptomyces isolates were recovered from 54 soil samples collected from 28 different locations in Jordan. These were then characterized by conventional methods and assessed for their activity against two antibiotic-resistant Gram-negative isolates of Escherichia coli and Klebsiella pneumoniae. Results revealed that grey, white and yellow series isolates were the most abundant, with 15% of the Streptomyces isolates active against at least one of the test pathogens. Most of the active isolates exhibited activity against E. coli (96%), while less activity was exhibited against K. pneumoniae (18%). Overall screening revealed the characterization of six Streptomyces isolates (I7, AC32, G17, Z11, Bb36 and AQ16) which inhibited the growth of both pathogens. All were obtained from a region characterized by low-nutrient soils and harsh conditions. The unusual antibiotic profile of these isolates stressed their potential as a source of novel antibiotics.
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References
Abdulla, K.A. 1998 Distribution of Streptomyces colour series in some non cultivated Iraqi soils and their antimicrobial activity against gram-negative bacteria. MSc Thesis, Yarmouk University, Jordan.
Bauer, A.W., Kirby, W.M., Sherris, J.C. & Turk, M. 1966 Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology 45, 493–496.
Blatz, R.H. 1998 Genetic manipulation of antibiotic producing Streptomyces. Trends in Microbiology 6, 76–83.
Demain, A.L. 1989 Functions of secondary metabolites. In Genetics and Molecular Biology of Industrial Microorganisms, eds. Hershberger, C.L., Queener, S.W. Hegema, G. pp. 1–11. Washington, D.C. ASM Press, ISBN 1-55581-063-2.
El-nakeeb, M.A. & Lechevalier, H.A. 1963 Selective isolation of aerobic actinomycetes. Applied Microbiology 11, 75–77.
Goodfellow, M. & Simpson, K.E. 1987. Ecology of streptomycete. Frontiers in Applied Microbiology 2, 97–125.
Hamdi, Y.A., Ahmed, D. & Al-tai, A. 1981 Genera and species of actinomycetes isolated from Iraqi soils. Egyptian Journal of Microbiology 15, 7–22.
Katsifas, E.A., Giannoutsou, E.P. & Karagouni, A.D. 1999 Diversity of streptomycetes among speci.c Greek terrestrial ecosystems. Letters in Applied Microbiology 29, 48–51.
Konishi, M., Okuma, H. & Kawaguchi, H. 1991 Dynamics new antibiotics with the 1,5 diyn-3-ene and anthraquinone subunit. Production, isolation and physico-chemical properties. Journal of Antibiotics 44, 1300–1305.
Kuster, E. & Williams, S.T. 1964 Selection of media for isolation of streptomycetes. Nature 202, 928–929.
Labeda, D.P. & Shearer, M.C. 1990 Isolation of actinomycetes for biotechnological applications. In Isolation of Biotechnological Organisms from Nature ed. Labeda, D.P. pp. 1–19. McGraw-Hill Publishing Company. ISBN 0-07-035701-3.
Martin, J. & Demain, A.L. 1980 Control of antibiotic biosynthesis. Microbiological Reviews 44, 230–251.
Mesameh, Y.M. 1992 Streptomyces in Jordan: Distribution and antibiotic activity. MSc Thesis, Yarmouk University, Jordan.
Nonomura, H. 1974 Key for classi.cation and identi.cation of 485 species of the streptomycetes included in the ISP. Journal of Fermentation Technology 52, 78–92.
Prauser, H. 1964 Aptness and application of colour for exact description of colours of Streptomyces. Zeitschrift fur Allgemeine Microbiologie 4, 95–98.
Saadoun, I. & Al-momani, F. 1996 Bacterial and Streptomyces flora of some Jordan Valley soils. Actinomycetes 7, 95–99.
Saadoun, I. & Al-momani, F. 1997 Studies on soil Streptomycetes from Jordan. Actinomycetes 8, 42–48.
Saadoun, I., Al-momani, F., Malkawi, H. & Mohammad, M.J. 1999 Isolation, identification and analysis of antibacterial activity of soil streptomycetes isolates from North Jordan. Microbios 100, 41–46.
Saadoun, I., Mohammad, M.J., Malkawi, H.I., Al-momani, F & Meqdam, M. 1998 Diversity of soil streptomycetes in Northern Jordan. Actinomycetes 9, 52–60.
Sanglier, J.J., Haag, H., Huck, T.A. & Fehr, T. 1993 Novel bioactive compounds from actinomycetes. Research Microbiology 144, 633–642.
Seto, H., Fujioka, T., Furihatha, K., Kaneko, J. & Takahkashi, S. 1989 Structure of complestain a very strong inhibitor of protease activity of complement in the human complement system. Tetrahedron Letters 37, 4987–4990.
Shirling, E.B. & Gottlieb, D. 1966 Methods for characterization of Streptomyces species. International Journal of Systematic Bacteriology 16, 313–340.
Takizawa, M.R., Colwell, R. & Hill R.T. 1993 Isolation and diversity of actinomycetes in the Chesapeake Bay. Applied and Environmental Microbiology 59, 997–1002.
Williams, S.T., Shameemullah, M., Watson, E.T. & Mayfield, C.I. 1972 Studies on the ecology of actinomycetes in soil VI. The influence of moisture tension on growth and survival. Soil Biology and Biochemistry 4, 215–225.
Williams, S.T., Goodfellow M., Alderson G., Wellington, E.M., Sneath P.H. & Sackin, M.J. 1983 Numerical classi.cation of Streptomyces and related genera. Journal of General Microbiology 129, 1743–1813.
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Saadoun, I., Gharaibeh, R. The Streptomyces flora of Jordan and its' potential as a source of antibiotics active against antibiotic-resistant Gram-negative bacteria. World Journal of Microbiology and Biotechnology 18, 465–470 (2002). https://doi.org/10.1023/A:1015531205871
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DOI: https://doi.org/10.1023/A:1015531205871