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Improved product formation in high density Chinese hamster ovary cell cultures transfected at confluency

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Abstract

Cell-specific productivity was compared in Chinese hamster ovary cell clones transfected at confluency versus subconfluency. Following lipofection of a pCDNA1-derived vector encoding a proteinA-fucosyltransferaseIV fusion protein, selection and single cell sorting by flow cytometry, clones were expanded to tissue flasks and assessed for cell specific productivity. The probability of obtaining high producers at confluency was significantly higher from cells transfected at confluency than at subconfluency (P=0.05).

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Authors and Affiliations

  1. Institute of Biotechnology, Research Center Jülich, Jülich, Germany

    Thomas Noll, Detlef Eisenkrätzer, André Kiesewetter, Christian Wandrey & Manfred Biselli

  2. Pharma Biotech Development & Production, Animal Cell Fermentation, Bldg. 352/563, Roche Diagnostics GmbH, Nonnenwald 2, D-82377, Penzberg, Germany

    Detlef Eisenkrätzer

  3. Institute of Physiology, University of Zürich, Zürich, Switzerland

    André Dinter, Steffen Zeng & Eric G. Berger

  4. Biochemie GmbH, R&D – Bioproducts, Cell Culture Process Development, Biochemiestrasse 10, A-6250, Kundl/Tirol, Austria

    Steffen Zeng

  5. University of Applied Sciences Aachen, Ginsterweg 1, D-52428, Jülich, Germany

    Manfred Biselli

Authors
  1. Thomas Noll
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  2. Detlef Eisenkrätzer
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  3. André Kiesewetter
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  4. André Dinter
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  5. Steffen Zeng
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  6. Christian Wandrey
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  7. Manfred Biselli
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  8. Eric G. Berger
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Noll, T., Eisenkrätzer, D., Kiesewetter, A. et al. Improved product formation in high density Chinese hamster ovary cell cultures transfected at confluency. Biotechnology Letters 24, 861–866 (2002). https://doi.org/10.1023/A:1015512607352

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