Abstract
The addition of menadione into the medium during cultivation ofNeurospora crassa in the dark activated its constitutive superoxide dismutase. Exposure to light not only activated superoxide dismutase and catalase, but also increased the content of neurosporaxanthin. Superoxide dismutase activity in the mixed (+/–) cultures of Blakeslea trispora synthesizing β-carotene in the dark was much lower than that inNeurospora crassa. The superoxide dismutase activity and catalase activity further decreased in oxidative stress with a parallel increase in the content of β-carotene. Our results indicate that neurosporaxanthin possesses photoprotective properties in Neurospora crassa. In Blakeslea trispora (+/–) fungi, β-carotene acts as a major antioxidant during inactivation of enzymes that detoxify reactive oxygen species.
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REFERENCES
Bennett, J.W., Secondary Metabolism and Differentiation in Fungi, Bennett, J.W. and Ciegler, A, Eds., New York: Marcel Dekker, 1983, pp. 1-32.
Hansberg, W. and Aguirre, J., J. Theor. Biol., 1990, vol. 142, pp. 201-221.
Anisimov, V.N., Usp. Sovrem. Biol., vol. 120, no. 2, pp. 146-164.
Feofilova, E.P., Pigmenty mikroorganizmov (Microbial Pigments), Moscow: Nauka, 1974.
Ruddat, M. and Garber, E.D., Secondary Metabolism and Differentiation in Fungi, Bennett, J.W. and Ciegler, A., Eds., New York: Marcel Dekker, 1983, pp. 95-152.
Johnson, E.A. and Schroeder, W.A., Adv. Biochem. Engin. Biotechnol., 1996, vol. 53, pp. 119-175.
DeFabo, E.C., Harding, R.W., and Shropshire, W., Plant Physiol., 1976, vol. 57, pp. 440-445.
Tereshina, V.M., Kiseleva, A.I., Feofilova, E.P., and Kashporova, E.V., Mikrobiologiya, 1993, vol. 62, no. 1, p. 56.
Vogel, H.J., Nature (London), 1964, vol. 98, pp. 435-446.
Belozerskaya, T.A., Kritskii, M.S., Levina, N.N., et al., Biologicheskie Membrany, 1988, vol. 5, pp. 1081-1089.
Bannister, J.V. and Bannister, W.H., Methods Enzymology,1984, vol. 105, pp. 88-93.
Kostyuk, V.A., Potapovich, A.I., and Kovaleva, Zh.V., Vopr. Med. Khimii, 1990, vol. 36, no. 2, pp. 88-91.
Eldred, G.E. and Hoffert, J.R., Anal. Biochem., 1981, vol. 110, no. 1, pp. 137-143.
Belozerskii, A.N., Prakticheskoe rukovodstvo po biokhimii rastenii (Practical Guide in Plant Biochemistry), Moscow: Sovetskaya Nauka, 1951, p. 281.
Beauchamp, C. and Fridovich, I., Anal. Biochem., 1971, vol. 44, pp. 276-287.
Lledias, S.F., Rangel, P., and Hansberg, W., J. Biol. Chem., 1998, vol. 273, no. 17, pp. 10 630-10 637.
Chary, P., Dillon, D., Schroeder, A.L., and Natvig, D.O., Genetics, 1994, vol. 137, pp. 723-730.
Natvig, D.O., Sylvester, K., Dvorachek, W.H., and Baldwin, J.L., The Mycota, Brambl, R. and Marzluf, G., Eds., Berlin: Springer, 1996, pp. 191-209.
Henry, L.E.A., Cammack, R., Schwitzguebel, J.P., et al., Biochem. J., 1980, vol. 187, pp. 321-328.
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Gessler, N.N., Sokolov, A.V., Bykhovsky, V.Y. et al. Superoxide Dismutase and Catalase Activities in Carotenoid-Synthesizing Fungi Blakeslea trispora and Neurospora crassa Fungi in Oxidative Stress. Applied Biochemistry and Microbiology 38, 205–209 (2002). https://doi.org/10.1023/A:1015454921023
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DOI: https://doi.org/10.1023/A:1015454921023