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Sugar Alcohols Enhance Calcium Transport from Rat Small and Large Intestine Epithelium In Vitro

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Abstract

We compared the effect of a variety of sugar alcohols on calcium absorption from the rat small and large intestine in vitro. An Ussing chamber technique was used to determine the net transport of Ca across the epithelium isolated from the jejunum, ileum, cecum, and colon of rats. The concentration of Ca in the serosal and mucosal Tris buffer solution was 1.25 mM and 10 mM, respectively. The Ca concentration in the serosal medium was determined after incubation for 30 min and the net Ca absorption was evaluated. The addition of 0.1–200 mM erythritol, xylitol, sorbitol, maltitol, palatinit, or lactitol to the mucosal medium affected net Ca absorption in the intestinal preparations. Differences in Ca transport were observed between portions of the intestine, but not between sugar alcohols tested. We concluded that sugar alcohols directly affect the epithelial tissue and promote Ca absorption from the small and large intestine in vitro.

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REFERENCES

  1. Dills WL Jr: Sugar alcohols as bulk sweeteners. Annu Rev Nutr 9:161–186, 1989

    PubMed  Google Scholar 

  2. Noda K, Oku T: Metabolism and disposition of erythritol after oral administration to rats. J Nutr 122:1266–1272, 1992

    PubMed  Google Scholar 

  3. Yoshizawa S, Moriuchi S, Hosoya N: The effects of maltitol on rat intestinal disaccharidases. J Nutr Sci Vitaminol (Tokyo) 21:31–37, 1975

    PubMed  Google Scholar 

  4. Nilsson U, Jagerstad M: Hydrolysis of lactitol, maltitol, and palatinit by human intestinal biopsies. Br J Nutr 58:199–206, 1987

    PubMed  Google Scholar 

  5. Hamalainen MM, Makinen KK, Parviainen MT, Koskinen T: Peroral xylitol increases intestinal calcium absorption in the rat independently of vitamin D action. Miner Electrolyte Metab 11:178–181, 1985

    PubMed  Google Scholar 

  6. Brommage R, Binacua C, Antille S, Carrie AL: Intestinal calcium absorption in rats is stimulated by dietary lactulose and other resistant sugars. J Nutr 123:2186–2194, 1993

    PubMed  Google Scholar 

  7. Goda T, Yamada M, Takase S, Hosoya N: Effect of maltitol intake on intestinal calcium absorption in the rat. J Nutr Sci Vitaminol (Tokyo) 38:277–286, 1992

    PubMed  Google Scholar 

  8. Goda T, Takase S, Hosoya N: Maltitol-induced increase of transepithelial transport of calcium in rat small intestine. J Nutr Sci Vitaminol (Tokyo) 39:589–595, 1993

    PubMed  Google Scholar 

  9. Kashimura J, Kimura M, Itokawa Y: The effects of isomaltulose, isomalt, and isomaltulose-based oligomers on mineral absorption and retention. Biol Trace Elem Res 54:239–250, 1996

    PubMed  Google Scholar 

  10. Ammann P, Rizzoli R, Fleisch H: Influence of the disaccharide lactitol on intestinal absorption and body retention of calcium in rats. J Nutr 118:793–795, 1988

    PubMed  Google Scholar 

  11. Hamalainen MM, Knuuttila M, Svanberg M, Koskinen T: Comparison of the effect of gluconate, lactose, and xylitol on bone recalcification in calcium-deficient rats. Bone 11:429–438, 1990

    PubMed  Google Scholar 

  12. Hamalainen MM: Retention of calcium from various xylitolcalcium combinations in rats. Proc Soc Exp Biol Med 205:253–256, 1994

    PubMed  Google Scholar 

  13. Goda T, Suruga K, Takase S, Ezawa I, Hosoya N: Dietary maltitol increases calcium content and breaking force of femoral bone in ovariectomized rats. J Nutr 125:2869–2873, 1995

    PubMed  Google Scholar 

  14. Goda T, Kishi K, Ezawa I, Takase S: The maltitol-induced increase in intestinal calcium transport increases the calcium content and breaking force of femoral bone in weanling rats. J Nutr 128:2028–2031, 1998

    PubMed  Google Scholar 

  15. Mattila PT, Svanberg MJ, Makinen KK, Knuuttila ML: Dietary xylitol, sorbitol and D-mannitol but not erythritol retard bone resorption in rats. J Nutr 126:1865–1870, 1996

    PubMed  Google Scholar 

  16. Egger B, Arnera V, Llull JB, Lauterburg BH: Effect of one month of lactitol treatment on calcium metabolism in man. Eur J Clin Pharmacol 37:205–207, 1989

    PubMed  Google Scholar 

  17. Ohta A, Ohtuki M, Takizawa T, Inaba H, Adachi T, Kimura S: Effects of fructooligosaccharides on the absorption of magnesium and calcium by cecectomized rats. Int J Vitam Nutr Res 64:316–323, 1994

    PubMed  Google Scholar 

  18. Suzuki T, Hara H, Kasai T, Tomita F: Effects of difructose anhydride III on calcium absorption in small and large intestines of rats. Biosci Biotechnol Biochem 62:837–841, 1998

    PubMed  Google Scholar 

  19. Saito K, Hira T, Suzuki T, Hara H, Yokota A, Tomita F: Effects of DFA IV in rats: Calcium absorption and metabolism of DFA IV by intestinal microorganisms. Biosci Biotechnol Biochem 63:655–661, 1999

    PubMed  Google Scholar 

  20. Hara H, Nagata M, Ohta A, Kasai T: Increases in calcium absorption with ingestion of soluble dietary fibre, guar-gum hydrolysate, depend on the caecum in partially nephrectomized and normal rats. Br J Nutr 76:773–784, 1996

    PubMed  Google Scholar 

  21. Hara H, Suzuki T, Aoyama Y: Ingestion of the soluble fiber, polydextrose, increases calcium absorption and bone mineralization in normal and total-gastrectomized rats. Br J Nutr 84:1–8, 2000

    PubMed  Google Scholar 

  22. Remesy C, Levrat MA, Gamet L, Demigne C: Cecal fermentations in rats fed oligosaccharides (inulin) are modulated by dietary calcium level. Am J Physiol 264:G855–G862, 1993

    PubMed  Google Scholar 

  23. Levrat MA, Remesy C, Demigne C: High propionic acid fermentations and mineral accumulation in the cecum of rats adapted to different levels of inulin. J Nutr 121:1730–1737, 1991

    PubMed  Google Scholar 

  24. Younes H, Demigne C, Remesy C: Acidic fermentation in the caecum increases absorption of calcium and magnesium in the large intestine of the rat. Br J Nutr 75:301–314, 1996

    PubMed  Google Scholar 

  25. Karbach U, Feldmeier H: The cecum is the site with the highest calcium absorption in rat intestine. Dig Dis Sci 38:1815–1824, 1993

    PubMed  Google Scholar 

  26. Chonan O, Matsumoto K, Watanuki M: Effect of galactooligosaccharides on calcium absorption and preventing bone loss in ovariectomized rats. Biosci Biotechnol Biochem 59:236–239, 1995

    PubMed  Google Scholar 

  27. Hara H, Haga S, Kasai T, Kiriyama S: Fermentation products of sugar-beet fiber by cecal bacteria lower plasma cholesterol concentration in rats. J Nutr 128:688–693, 1998

    PubMed  Google Scholar 

  28. Dupuis Y, Digaud A, Fournier P: The relations between intestinal alkaline phosphatase and carbohydrates with regard to calcium absorption. Arch Int Physiol Biochim 86:543–556, 1978

    PubMed  Google Scholar 

  29. Fukahori M, Sakurai H, Akatsu S, Negishi M, Sato H, Goda T, Takase S: Enhanced absorption of calcium after oral administration of maltitol in the rat intestine. J Pharm Pharmacol 50:1227–1232, 1998

    PubMed  Google Scholar 

  30. Kishi K, Goda T, Takase S: Maltitol increases transepithelial diffusional transfer of calcium in rat ileum. Life Sci 59:1133–1140, 1996

    PubMed  Google Scholar 

  31. Lacour B, Ohan J, Aznag A, Drueke TB: Stimulating effects of sorbitol and L-xylose on rat ileal Ca transport in vitro. Miner Electrolyte Metab 21:391–397, 1995

    PubMed  Google Scholar 

  32. Hidalgo IJ, Hillgren KM, Grass GM, Borchardt RT: Characterization of the unstirred water layer in Caco-2 cell monolayers using a novel diffusion apparatus. Pharm Res 8:222–227, 1991

    PubMed  Google Scholar 

  33. Duflos C, Bellaton C, Pansu D, Bronner F: Calcium solubility, intestinal sojourn time and paracellular permeability codetermine passive calcium absorption in rats. J Nutr 125:2348–2355, 1995

    PubMed  Google Scholar 

  34. Ferraris RP, Yasharpour S, Lloyd KC, Mirzayan R, Diamond JM: Luminal glucose concentrations in the gut under normal conditions. Am J Physiol 259:G822–G837, 1990

    PubMed  Google Scholar 

  35. Bronner F: Calcium absorption—a paradigm for mineral absorption. J Nutr 128:917–920, 1998

    PubMed  Google Scholar 

  36. Bronner F, Pansu D: Nutritional aspects of calcium absorption. J Nutr 129:9–12, 1999

    PubMed  Google Scholar 

  37. Ballard ST, Hunter JH, Taylor AE: Regulation of tightjunction permeability during nutrient absorption across the intestinal epithelium. Annu Rev Nutr 15:35–55, 1995

    PubMed  Google Scholar 

  38. Nusrat A, Turner JR, Madara JL: IV. Regulation of tight junctions by extracellular stimuli: Nutrients, cytokines, and immune cells [In Process Citation]. Am J Physiol. 279:G851–G857, 2000

    Google Scholar 

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Authors and Affiliations

  1. Hokkaido Foundation for the Promotion of Scientific and Industrial Technology, Colabo-Hokkaido, 001-0021, Sapporo, Japan

    Hitoshi Mineo

  2. Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan

    Hiroshi Hara & Fusao Tomita

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  1. Hitoshi Mineo
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  2. Hiroshi Hara
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  3. Fusao Tomita
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Mineo, H., Hara, H. & Tomita, F. Sugar Alcohols Enhance Calcium Transport from Rat Small and Large Intestine Epithelium In Vitro . Dig Dis Sci 47, 1326–1333 (2002). https://doi.org/10.1023/A:1015378732294

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