Abstract
The accuracy and reliability of diagnostic tests for infections and cancer can be substantially improved by using a gel, rather than a liquid medium, to amplify nucleic acids and thereby to obtain molecular colonies.
Similar content being viewed by others
REFERENCES
Koch, R., Mittheilungen aus dem Kaiserlichen Gesundheitsamte, 1881, vol. 1, pp. 1-48.
Saiki, R.K., Scharf, S., Faloona, F., et al., Science, 1985, vol. 230, pp. 1350-1354.
Haruna, I. and Spiegelman, S., Science, 1965, vol. 150, pp. 884-886.
Chetverin, A.B. and Spirin, A.S., Prog. Nucleic Acid Res. Mol. Biol., 1995, vol. 51, pp. 225-270.
Chetverin, A.B., Usp. Biol. Khim., 1998, vol. 38, pp. 375.
Brown, D. and Gold, L., Biochemistry, 1995, vol. 34, pp. 14 775-14 782.
Lizardi, P.M., Guerra, C.E., Lomeli, H., et al., Bio/Technology, 1988, vol. 6, pp. 1197-1202.
Lomeli, H., Tyagi, S., Pritchard, C.G., et al., Clin. Chem., 1989, vol. 35, pp. 1826-1831.
Tyagi, S., Landgren, U., Tazi, M., et al., Proc. Natl. Acad. Sci. USA, 1996, vol. 93, pp. 5395-5400.
Taylor, G.R. and Robinson, P., Curr. Opin. Biotechnol., 1998, vol. 9, pp. 35-42.
Panet, A. and Khorana, H.G., J. Biol. Chem., 1974, vol. 249, pp. 5213-5221.
Saiki, R.K., Gelfand, D.H., Stoffel, S., et al., Science, 1988, vol. 239, pp. 487-491.
Myers, T.W. and Gelfand, D.H., Biochemistry, 1991, vol. 30, pp. 7661-7666.
Wu, D.Y. and Wallace, R.B., Genomics, 1989, vol. 4, pp. 560-569.
Barany, F., Proc. Natl. Acad. Sci. USA, 1991, vol. 88, pp. 189-193.
Guatelli, J.C., Whitfield, K.M., Kwoh, D.Y., et al., Proc. Natl. Acad. Sci. USA, 1990, vol. 87, pp. 1874-1878.
Compton, J., Nature, 1991, vol. 350, pp. 91-92.
Fahy, E., Kwoh, D.Y., and Gingeras, T.R., PCR Methods Appl., 1991, vol. 1, pp. 25-33.
Walker, G.T., Little, M.C., Nadeau, J.G., and Shank, D.D., Proc. Natl. Acad. Sci. USA, 1992, vol. 89, pp. 392-396.
Zimmermann, K. and Mannhalter, J.W., BioTechniques, 1996, vol. 21, pp. 268-279.
Zazzi, M., Romano, L., Catucci, M., et al., J. Clin. Microbiol., 1995, vol. 33, pp. 205-208.
Barlow, K.L., Tosswill, J.H., Parry, J.V., and Clewley, J.P., J. Clin. Microbiol., 1997, vol. 35, pp. 2846-2853.
Freeman, W.M., Walker, S.J., and Vrana, K.E., BioTechniques, 1999, vol. 26, pp. 112-125.
Chetverin, A.B. and Chetverina, E.V., RF Patent 2048522, 1995.
Chetverin, A.B. and Chetverina, E.V., RF Patent 2114175, 1998.
Chetverin, A.B. and Chetverina, E.V., RF Patent 2114915, 1998.
Chetverin, A.B. and Chetverina, H.V., US Patent 5616478, 1997.
Chetverin, A.B. and Chetverina, H.V., US Patent 5958698, 1999.
Chetverin, A.B. and Chetverina, H.V., US Patent 6001568, 1999.
Sumper, M. and Luce, R., Proc. Natl. Acad. Sci. USA, 1975, vol. 72, pp. 162-166.
Biebricher, C.K., Eigen, M., and Luce, R., Nature, 1986, vol. 321, pp. 89-91.
Munishkin, A.V., Voronin, L.A., and Chetverin, A.B., Nature, 1988, vol. 333, pp. 473-475.
Chetverin, A.B., Chetverina, H.V., and Munishkin, A.V., J. Mol. Biol., 1991, vol. 222, pp. 3-9.
Chetverin, A.B., Mol. Biol., 1996, vol. 30, pp. 981-990.
Chetverina, H.V. and Chetverin, A.B., Nucleic Acids Res., 1993, vol. 21, pp. 2349-2353.
Chetverin, A.B., Chetverina, H.V., Demidenko, A.A., and Ugarov, V.I., Cell, 1997, vol. 88, pp. 503-513.
Chetverina, H.V., Demidenko, A.A., Ugarov, V.I., and Chetverin, A.B., FEBS Lett., 1999, vol. 450, pp. 89-94.
Mitra, R.D. and Church, G.M., Nucleic Acids Res., 1999, vol. 27, p. e34.
Myers, T.W. and Sigua, C.L., PCR Strategies, Innis, M.A., Gelfand, D.H., and Sninsky, J.J., Eds., San Diego, CA: Academic, 1994, pp. 58-68.
Tyagi, S. and Kramer, F.R., Nat. Biotechnol., 1996, vol. 14, pp. 303-308.
Sidransky, D., Science, 1997, vol. 278, pp. 1054-1058.
Vogelstein, B. and Kinzler, K.W., Proc. Natl. Acad. Sci. USA, 1999, vol. 96, pp. 9236-9241.
Abelev, G.I. and Eraiser, T.L., Semin. Cancer Biol., 1999, vol. 9, pp. 95-107.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chetverin, A.B., Chetverina, H.V. Accurate Diagnostics with the Use of Molecular Colonies. Molecular Biology 36, 244–250 (2002). https://doi.org/10.1023/A:1015378108109
Issue Date:
DOI: https://doi.org/10.1023/A:1015378108109