Abstract
The complete nucleotide sequence of Cucurbit yellow stunting disorder virus (CYSDV) RNA 2, a whitefly (Bemisia tabaci)-transmitted closterovirus with a bi-partite genome, is reported. CYSDV RNA 2 is 7,281 nucleotides long and contains the closterovirus hallmark gene array with a similar arrangement to the prototype member of the genus Crinivirus, Lettuce infectious yellows virus (LIYV). CYSDV RNA 2 contains open reading frames (ORFs) potentially encoding in a 5′ to 3′ direction for proteins of 5 kDa (ORF 1; hydrophobic protein), 62 kDa (ORF 2; heat shock protein 70 homolog, HSP70h), 59 kDa (ORF 3; protein of unknown function), 9 kDa (ORF 4; protein of unknown function), 28.5 kDa (ORF 5; coat protein, CP), 53 kDa (ORF 6; coat protein minor, CPm), and 26.5 kDa (ORF 7; protein of unknown function). Pairwise comparisons of CYSDV RNA 2-encoded proteins (HSP70h, p59 and CPm) among the closteroviruses showed that CYSDV is closely related to LIYV. Phylogenetic analysis based on the amino acid sequence of the HSP70h, indicated that CYSDV clusters with other members of the genus Crinivirus, and it is related to Little cherry virus-1 (LChV-1), but is distinct from the aphid- or mealybug-transmitted closteroviruses.
Similar content being viewed by others
References
Martelli G.P., Agranovsky A.A., Bar-Joseph M., Boscia D., Candresse T., Coutts R.H.A., Dolja V.V., Duffus J.E., Falk B.W., Gonsalves D., Jelkmann W., Karasev A.V., Minafra A., Murant A., Namba S., Niblett C.L., Vetten H.J., and Yoshikawa N., in van Regenmortel M.H.V., Fauquet C.M., Bishop D.H.L., Carstens E.B., Estes M.K., Lemon S.M., Maniloff J., Mayo M.A., McGeoch D.J., Pringle C.R., and Wickner R.B. (eds), Virus Taxonomy, 7th Report of the International Committee on Taxonomy of Viruses, Academic Press, San Diego, California, USA, 2000, 949-952.
Karasev A.V., Annu Rev Phytopath 38, 293-324, 2000.
Livieratos I.C. and Coutts R.H.A., in Tidona C.A., and Darai G. (eds), The Springer Index of Viruses, Springer Verlag, Heidelberg, Germany, 2002.
Woudt L.P., de Rover A.P., de Haan P.T., and van Grinsven M.Q.J.M., Proceedings of 6th International Congress Virology, Glasgow, Scotland, 1993.
Liu H.-Y. and Duffus J.E., Phytopathol 80, 866-869, 1990.
Coffin R.S. and Coutts R.H.A., J Phytopathol 143, 375-380, 1995.
Liu H.-Y., Wisler G.C. and Duffus J.E., Plant Dis 84, 803-805, 2000.
Klaassen V.A., Boeshore M.L., Koonin E.V., Tian T., and Falk B.W., Virology 208, 99-110, 1995.
Yeh H.-H., Tian T., Rubio L., Crawford B., and Falk B.W., J Virol 74, 5762-5768, 2000.
Hassan A.A. and Duffus J.E., Emir J Agric Sci 2, 1-16, 1991.
Celix A., Lopez-Sese A., Almarza N., Gomez-Guillamon M.L., and Rodriguez-Cerezo E., Phytopathology 86, 1370-1376, 1996.
Berdiales B., Bernal J.J., Saez E., Woudt B., Beitia F., and Rodriguez-Cerezo E., Eur J Plant Path 105, 211-215, 1999.
Rubio L., Soong J., Kao J., and Falk B.W., Phytopathology 89, 707-711, 1999.
Kao J., Jia L., Tian T., Rubio L., and Falk B.W., Plant Dis 89, 101, 2000.
Livieratos I.C., Avgelis A.D., and Coutts R.H.A., Phytopathology 89, 1050-1055, 1999.
Tian T., Klaassen V.A., Soong J., Wisler G., Duffus J.E., and Falk B.W., Phytopathology 86, 1167-1173, 1996.
Livieratos I.C., Katis N., and Coutts R.H.A., Plant Path 47, 362-369, 1998.
Bringloe D.H., Gultyaev A.P., Pelpel M., Pleij C.W.A., and Coutts R.H.A., J Gen Virol 79, 1539-1546, 1998.
Liu X. and Gorovsky M.A., Nucleic Acids Res 21, 4954-4960, 1993.
Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Miller W., and Lipman D.J., Nucleic Acids Res 25, 3389-3402, 1997.
Deveraux J., Haeberli P., and Smithies O., Nucleic Acids Res 12, 387-395, 1984.
Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., and Higgins D.G., Nucleic Acids Res 25, 4876-4882, 1997.
Page R.D.M., Comp Appl Biosci 12, 357-358, 1996.
Rubio L., Yeh H.-H., Tian T., and Falk B.W., Virology 271, 205-212, 2000.
Mawassi M.E., Mietkiewska M.E., Hilf L., Ashoulin A., Karasev V., Gafny R., Lee R.F., Garnsey S.M., Dawson W.O., and Bar-Joseph M., Virology 214, 264-268, 1995.
Wisler G.C., Li R.H., Liu H.-Y., Lowry D.S., and Duffus J.E., Phytopathol 88, 402-409, 1998.
Hoyer U., Maiss E., Jelkmann W., Lesemann D.E., and Vetten H.J., Phytopathol 86, 744-750, 1996.
Wisler G.C., Liu H.-Y., Klaassen V.A., Duffus J.E., and Falk B.W., Phytopathol 86, 622-626, 1996.
Salazar L.F., Muller G., Querci M., Zapata J.L., and Owens R.A., Ann Appl Biol 137, 7-19, 2000.
Ting J. and Lee A.S., DNA 7, 275-286, 1988.
Satyanarayana T., Gowda S., Mawassi M., Albiach-Marti M.R., Ayllon M.A., Robertson C., Garnsey S.M., and Dawson W.O., Virology 278, 253-265, 2000.
Alzhanova D.V., Hagiwara Y., Peremyslov V. V., and Dolja V.V., Virology 268, 192-200, 2000.
Agranovsky A.A., Adv Virus Res 47, 119-158, 1996.
Jelkmann W., Fechtner B., and Agranovsky A.A., J Gen Virol 78, 2067-2071, 1997.
Medina V., Tian T., Yeh H-H., Livieratos I.C., and Falk B.W., Proceedings of the 20th Conference of ASV, Madison, USA, Vol. 139, 2001.
Rott M.E. and Jelkmann W., Phytopathol 91, 261-267, 2001.
Eastwell K.C. and Bernardy M.G., Phytopathol 91, 268-273, 2001.
Zhu H.-Y., Ling K.S., Goszcynski D.E., McFerson J.R., and Gonsalves D., J Gen Virol 79, 1289-1298, 1998.
Karasev A.V., Nikolaeva O.V., Mushegian A.R., Lee R.F., and Dawson W.O., Virology 221, 199-207, 1996.
Melzer M.J., Karasev A.V., Sether D.M., and Hu J.S., J Gen Virol 82, 1-7, 2001.
Fazeli C.F. and Rezaian M.A., J Gen Virol 81, 605-615, 2000.
Ling K.S., Zhu H.Y., Drong R.F., Slightom J.L., McFerson J.R., and Gonsalves D., J Gen Virol 79, 1299-1307, 1998.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Livieratos, I.C., Coutts, R.H.A. Nucleotide Sequence and Phylogenetic Analysis of Cucurbit Yellow Stunting Disorder Virus RNA 2. Virus Genes 24, 225–230 (2002). https://doi.org/10.1023/A:1015372331039
Issue Date:
DOI: https://doi.org/10.1023/A:1015372331039