Abstract
The 3a protein of Cucumber mosaic virus is essential for the cell-to-cell movement of the viral RNA through plasmodesmata. We have introduced an epitope peptide before the stop codon of the 3a protein and cloned the tagged ORF into a binary vector for Agrobacterium-mediated transformation. The established transgenic tobacco lines produced the 3a protein, which was specifically detected with anti-3a and anti-epitope antisera. Metabolic labeling and subsequent immunoprecipitation revealed that [32P]-orthophosphate was incorporated into the 3a protein. The phosphoamino acid analysis indicated that the 3a protein contained phosphoserine but not phosphothreonine or phosphotyrosine. This is the first demonstration of the 3a protein phosphorylation in planta.
Similar content being viewed by others
References
Canto T., Prior D.A., Hellwald K.H., Oparka K.J., and Palukaitis P., Virology 237, 237-248, 1997.
Takahashi H., Suzuki M., Natsuaki K., Shigyo T., Hino K., Teraoka T., Hosokawa D., and Ehara Y., Plant Cell Physiol 42, 340-347, 2001.
Reichel C., Más P., and Beachy R.N., Trends Plant Sci 4, 458-462, 1999.
Matsushita Y., Deguchi M., Youda M., Nishiguchi M., and Nyunoya H., Mol Cells 12, 57-66, 2001.
Atkins D., Roberts K., Hull R., Prehaud C., and Bishop D.H., J Gen Virol 72, 2831-2835, 1991.
Watanabe Y., Ogawa T., and Okada Y., FEBS Lett 313, 181-184, 1992.
Citovsky V., McLean B.G., Zupan J.R., and Zambryski P., Genes Dev 7, 904-910, 1993.
Haley A., Hunter T., Kiberstis P., and Zimmern D., Plant J 8, 715-724, 1995.
Kawakami S., Padgett H.S., Hosokawa D., Okada Y., Beachy R.N., and Watanabe Y., J Virol 73, 6831-6840, 1999.
Karpova O.V., Rodionova N.P., Ivanov K.I., Kozlovsky S.V., Dorokhov Y.L., and Atabekov J.G., Virology 261, 20-24, 1999.
Matsushita Y., Hanazawa K., Yoshioka K., Oguchi T., Kawakami S., Watanabe Y., Nishiguchi M., and Nyunoya H., J Gen Virol 81, 2095-2102, 2000.
Waigmann E., Chen M.-H., Bachmaier R., Ghoshroy S., and Citovsky V., EMBO J 19, 4875-4884, 2000.
Tacke E., Schmitz J., Prüfer D., and Rohde W., Virology 197, 274-282, 1993.
Sokolova M., Prüfer D., Tacke E., and Rohde W., FEBS Lett 400, 201-205, 1997.
Sato K., Yoshikawa N., Takahashi T., and Taira H., J Gen Virol 76, 1503-1507, 1995.
Hayakawa T., Mizukami M., Nakajima M., and Suzuki M., J Gen Virol 70, 499-504, 1989.
Gleave A.P., Plant Mol Biol 20, 1203-1207, 1992.
Takahashi H. and Ehara Y., Mol Plant Microbe Interact 6, 182-189, 1993.
Ding B., Li Q., Nguyen L., Palukaitis P., and Lucas W.J., Virology 207, 345-353, 1995.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Matsushita, Y., Yoshioka, K., Shigyo, T. et al. Phosphorylation of the Movement Protein of Cucumber Mosaic Virus in Transgenic Tobacco Plants. Virus Genes 24, 231–234 (2002). https://doi.org/10.1023/A:1015324415110
Issue Date:
DOI: https://doi.org/10.1023/A:1015324415110