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Pathways Involved in Mild Gastrointestinal Inflammation Induced by a Low Level Exposure to a Food Contaminant

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Abstract

Chronic gut inflammation is associated with radical oxygen species (ROS) genesis. ROS may activate certain transcription factors such as nuclear factor κ beta (NF-κB), which regulates cyclooxygenase-2 (COX-2). Diquat, a food contaminant, is responsible for oxidative stress. This work aimed to establish the involvement of ROS and prostanoids on diquat-induced gastrointestinal inflammation and mast cell hyperplasia. Diquat increased gastrointestinal MPO activity and mast cell number. Its effect on gastric MPO activity was reversed by PD 138,387 (a COX-2 selective inhibitor) and PDTC (an inhibitor of NF-κB activation) but not by DMSO (a hydroxyl radical scavenger) and allopurinol (a xanthine oxidase inhibitor). In contrast, increased jejunal MPO activity was blocked by both DMSO, PD 138,387, and PDTC, while allopurinol enhanced it. PD 138,387 and PDTC reduced gastrointestinal mast cell number while DMSO and allopurinol did not Diquat-induced inflammation involves a gastrointestinal NF-κB activation and COX-2 dependent proinflammatory prostanoid synthesis. Furthermore, the hydroxyl radical is involved in intestinal but not gastric inflammation.

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Authors and Affiliations

  1. INRA, Neuro-Gastroenterology and Nutrition Unit, 180 chemin de Tournefeuille, BP3, 31931, Toulouse, France

    Pauline M. Anton, Vassilia Theodorou, Sabine Roy, Jean Fioramonti & Lionel Bueno

  2. Ecole Superieure d’Agriculture de Purpan, 75 voie du TOEC, 31076, Toulouse, France

    Pauline M. Anton

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  1. Pauline M. Anton
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  2. Vassilia Theodorou
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  4. Jean Fioramonti
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  5. Lionel Bueno
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Anton, P.M., Theodorou, V., Roy, S. et al. Pathways Involved in Mild Gastrointestinal Inflammation Induced by a Low Level Exposure to a Food Contaminant. Dig Dis Sci 47, 1308–1315 (2002). https://doi.org/10.1023/A:1015322514547

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