Abstract
Six kinds of heat-released soluble cell-wall fragments (elicitors) were prepared respectively from Neurospora crassa, Monascus purpureus, Sporobolomyces roseus, Rhodotorula rubra, Nocardia corallina N89 and Actinoplanes tuftoflagellus A05. When Penicillium sp. PT95 was grown on corn meal (CM) solid medium containing appropriate amounts of elicitors, both its sclerotia biomass and the amount of carotenoid accumulated in sclerotia were enhanced significantly (P < 0.01). Every one of the elicitors except that fromM. purpureus could also increase significantly the β-carotene fraction of total pigment (P < 0.01). Among elicitors tested, the elicitor (150 μg/g CM) originating from R. rubra gave a maximum value of sclerotia biomass, reaching 15.90 g/100g CM; the elicitor (100 μg/g CM) from M. purpureus gave the highest total carotenoid of 14,446 μg/100 g CM and β-carotene yield of 10,112 μg/100 g CM, which were respectively 2.76 and 2.72 times higher than that of control. Experimental results also showed that the elicitor from M. purpureus could inhibit effectively the occurrence of sectoring during solid-state fermentation of strain PT95.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abdalla, M.H. 1975 Sectoring of fungal colonies induced by low concentration of fernasan. Mycopathologia 56, 39–40.
An, G.H., Schuman, D.B. & Johnson, E.A. 1989 Isolation of Phaffa rhodozyma mutants with increased astaxanthin content. Applied Environmental Microbiology 55, 116–124.
Ayers, A.R., Ebel, J., Valent, B. & Albersheim, P. 1976 Host pathogen interactions - X. Fractionation and biological activity of an elicitor isolated from the mycelial walls of Phytophthora megasperma var. sojae. Plant Physiology 57, 760–765.
Chen, Y., Zhu, W. & Wu, Y. 1999 Effects of fungus elicitors on taxol production in suspension cells of Taxus yunnanensis. Chinese Journal of Biotechnology 15, 522–524.
Du, R.J. 1985 Biological Statistics. pp. 180–184. Beijing: Higher Education Press.
Ebel, J. & Scheel, D. 1992 Genes involved in plant defense, eds. Boller, T. & Meins, F., pp. 183-205. Vienna: Springer-Verlag Wien. ISBN3-21182312-3.
Goodwin, T.W. 1972 Carotenoids in fungi and non-photosynthetic bacteria. Progress in Industrial Microbiology 11, 31–88.
Han, J.R. 1998 Sclerotia growth and carotenoid production of Penicillium sp. PT95 during solid state fermentation of corn meal. Biotechnology Letters 20, 1063–1065.
Han, J.R., Wang, X.J. & Yuan, X.E. 1998 Studies on the production of carotenoids in sclerotia of PT95 strain of Penicillium. Microbiology 25, 319–321.
Hawker, L.E. 1956 The Physiology of Reproduction in Fungi. Cambridge: Cambridge University Press.
Salton, M.R.J. 1961 Microbial Cell Walls. New York: Wiley.
Schwacke, R. & Hager, A. 1992 Fungal elicitors induce a transient release of active oxygen species from cultured spruce cells that is dependent on Ca2+ and protein-kinase activity. Planta 187, 136–141.
Wang, Y.Q. & Li, Q.S. 1997 Advances in Natural Carotenoids. pp. 150–165. Beijing: Chinese Medicinal Science and Technology Press.
Wei, Z.M., Laby, R.L., Zumo., C.H., Baver, D.W., He, S-Y., Collmer, A. & Beer, S.V. 1992 Harpin, elicitor of the hypersensitive response produced by the plant pathogen Erwinia amylovoxa. Science 257, 85–88.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Han, JR., Gao, PP. Effect of several elicitors on sclerotia biomass and carotenoid yield of Penicillium sp. PT95 during solid-state fermentation of corn meal. World Journal of Microbiology and Biotechnology 18, 371–375 (2002). https://doi.org/10.1023/A:1015269322340
Issue Date:
DOI: https://doi.org/10.1023/A:1015269322340