Abstract
We recently tested three inbred mouse strains (C57BL/6J, DBA/2J, C3H/HeJ) and two F1 hybrids (B6C3F1/J, C3D2F1/J) in an elevated zero-maze for 3 consecutive days. As measured by the latency to enter an open quadrant and percentage of time spent in the open, anxiety increased over the three trials. Furthermore, we observed that some strains used visual cues to avoid the open arms of the zero-maze on the initial exposure, while other strains may have used other sensory cues. These results suggest that strains differentially use or retain information, gathered from the initial exposure, to avoid the open quadrants on subsequent exposure to the maze. Moreover, this repeated trial test may more accurately reflect anxiety in strains that are visually impaired.
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REFERENCES
Almeida, S. S., Garcia, R. A., and de Oliveira, L. M. (1993). Effects of protein malnutrition and repeated testing upon locomotor and exploratory behaviors in the elevated plus-maze. Phys. Behav. 54:749–752.
Bickerdike, M. J., Marsden, C. A., Dourish, C. T., and Fletcher, A. (1994). The influence of 5-hydroxytryptamine re-uptake blockade on CCK receptor antagonist effects in the rat elevated zeromaze. Eur. J. Pharm. 271:403–411.
Bolivar, V. J., Caldarone, B. J., Reilly, A. A., and Flaherty, L. (2000). Habituation of activity in an open field: A survey of inbred strains and F1 hybrids. Behav. Genet. 30:285–293.
Cook, M. N., Williams, R. W., and Flaherty, L. (2001). Anxietyrelated behaviors in the elevated zero-maze are affected by genetic factors and retinal degeneration. Behav. Neurosci. 115:468–476.
Espejo, E. F. (1997). Effects of weekly or daily exposure to the elevated plus-maze in male mice. Behav. Br. Res. 87:233–238.
Fernandes, C., and File, S. E. (1996). The influence of open arm ledges and maze experience in the elevated plus-maze. Pharmacol. Biochem. Behav. 54:31–40.
Hogg, S. (1996). A review of the validity and variability of the elevated plus-maze as an animal model of anxiety. Pharm. Biochem. Behav. 54:21–30.
Holmes, A., and Rodgers, R. J. (1998). Responses of swiss-webster mice to repeated plus-maze experience: further evidence for a qualitative shift in emotional state? Pharm. Biochem. Behav. 60: 473–488.
Menard, J., and Treit, D. (1999). Effects of centrally administered anxiolytic compounds on animal models of anxiety. Neurosci. Biobehav. Revs. 23:591–613.
Owen, E. H., Logue, S. F., Rasmussen, D. L., and Wehner, J. M. (1997). Assessment of learning by the Morris water task and fear conditioning in inbred mouse strains and F1 hybrids: Implications of genetic background for single gene mutations and quantitative trait loci analyses. Neuroscience 80:1087–1099.
Pellow, S., and File, S. E. (1986). Anxiolytic and anxiogenic drug effects on exploratory activity in an elevated plus-maze: A novel test of anxiety in the rat. J. Neurosci. Meth. 24:525–529.
Rodgers, R. J., Johnson, N. J. T., Cole, J. C., Dewar, C. V., Kidd, G. R., and Kimpson, P. H. (1996). Plus-maze retest profile in mice: Importance of initial stages of trial 1 and response to post-trial cholinergic receptor blockade. Pharm. Biochem. Behav. 54:41–50.
Rodgers, R. J., and Shepherd, J. K. (1993). Influence of prior maze experience on behaviour and response of diazepam in the elevated plus maze and light/dark tests. Psychopharmacology 113:237–242.
Shepherd, J. K., Grewal., S. S., Fletcher, A., Bill, D. J., and Dourish, C. T. (1994). Behavioral and pharmacological characterisation of the elevated “zero-maze” as an animal model of anxiety. Psychopharmacology 116:56–64.
Treit, D., Menard, J., and Royan, C. (1993). Anxiogenic stimuli in the elevated plus maze. Pharm. Biochem. Behav. 44:463–469.
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Cook, M.N., Crounse, M. & Flaherty, L. Anxiety in the Elevated Zero-Maze Is Augmented in Mice After Repeated Daily Exposure. Behav Genet 32, 113–118 (2002). https://doi.org/10.1023/A:1015249706579
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DOI: https://doi.org/10.1023/A:1015249706579