Abstract
Ferritin-binding proteins circulating in mammalian blood are thought to be involved in the clearance of ferritin. The present study characterizes canine serum autoantibodies (IgM and IgA) that react with ferritin. Canine IgM and IgA bound to bovine spleen ferritin as well as canine liver ferritin. To examine the specificity of canine IgM and IgA to ferritin H and L subunits, we used canine heart ferritin and canine liver ferritin with H/L subunit ratios of 3.69 and 0.43, respectively. Canine IgM and IgA recognized both of the H- and L-subunit-rich isoferritins, showing that their binding activities to ferritin depend on the H-subunit content. Recombinant bovine H-chain ferritin homopolymer expressed in a baculovirus expression system bound more with IgM and IgA than the recombinant L-chain homopolymer expressed under the same conditions. These results suggest that canine IgM and IgA recognize H-subunit-rich isoferritins, and that H-subunit-rich isoferritins are cleared from the circulation more rapidly than L-subunit-rich isoferritins.
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References
Addison GM, Beamish MR, Hales CN, Hodgkins M, Jacobs A, Llewellin P. 1972 An immunoradiometric assay for ferritin in the serum of normal subjects and patients with iron deficiency and iron overload. J Clin Pathol 25, 326-329.
Andrews GA, Smith JE, Gray M, Chavey PS, Weeks BR. 1992 An improved ferritin assay for canine sera. Vet Clin Pathol 21, 57-60.
Blight GD, Morgan EH. 1983 Ferritin and iron uptake by reticulocytes. Br J Haematol 55, 59-71.
Covell AM, Jacobs A, Worwood M. 1984 Interaction of ferritin with serum: implications for ferritin turnover. Clin Chim Acta 139, 75-84.
Cragg SJ, Wagstaff M, Worwood M. 1981 Detection of a glycosylated subunit in human serum ferritin. Biochem J 199, 565-571.
Davis BJ. 1964 Disc electrophoresis-II. Method and application to human serum proteins. Ann NY Acad Sci 121, 404-427.
Harrison PM, Arosio P. 1996 The ferritins: molecular properties, iron storage function and cellular regulation. Biochim Biophys Acta 1275, 161-203.
Kakuta K, Orino K, Yamamoto S, Watanabe K. 1997 High levels of ferritin and its iron in fetal bovine serum. Comp Biochem Physiol 118A, 165-169.
Lamparelli RDV, Friedman BM, MacPhail AP, Bothwell TH, Phillips JI, Baynes RD. 1989 The fate of intravenously injected tissue ferritin in pregnant guinea-pigs. Br J Haematol 72, 100-105.
Levi S, Luzzago A, Cesareni G, Cozzi A, Franceschinelli F, Albertini A, Arosio P. 1988 Mechanism of ferritin iron uptake: activity of the H-chain and deletion mapping of the ferro-oxidase site. A study of iron uptake and ferro-oxidase activity of human liver, recombinant H-chain ferritins, and of two H-chain deletion mutants. J Biol Chem 263, 18086-18092.
Levi S, Salfeld J, Franceshinelli F, Cozzi A, Dorner MH, Arosio P. 1989 Expression and structural and functional properties of human ferritin L-chain from Escherichia coli. Biochemistry 28, 5179-5184.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951 Protein measurement with the Folin phenol reagent. J Biol Chem 193, 265-275.
Luzzago A, Arosio P, Iacobello C, Ruggeri G, Capucci L, Brocchi E, de Simone F, Gamba D, Gabri E, Levi S, Albertini A. 1986 Immunochemical characterization of human liver and heart ferritins with monoclonal antibodies. Biochim Biophys Acta 872, 61-71.
Massover WH. 1994 α 2-Macroglobulin: a ferritin-binding protein. Ann N Y Acad Sci 737, 468-471.
Orino K, Eguchi K, Nakayama T, Yamamoto S, Watanabe K. 1997 Sequencing of cDNA clones that encode bovine ferritin H and L chains. Comp Biochem Physiol 118B, 667-673.
Orino K, Yamamoto S, Watanabe K. 1993 Fibrinogen as a ferritin-binding protein in horse plasma. J Vet Med Sci 55, 785-787.
Pollock AS, Lipschitz DA, Cook JD. 1978 The kinetics of serum ferritin. Proc Soc Exp Biol Med 157, 481-485.
Santambrogio P, Cozzi A, Levi S, Arosio P. 1987 Human serum ferritin G-peptide is recognized by anti-L ferritin subunit antibodies and concanavalin-A. Br J Haematol 65, 235-237.
Santambrogio P, Massover WH. 1989 Rabbit serum alpha-2-macroglobulin binds to liver ferritin: association causes a heterogeneity of ferritin molecules. Br J Haematol 71, 281-290.
Schägger H, von Jagow G. 1987 Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166, 368-379.
Smith JE, Moore K, Cipriano JE, Morris PG. 1984 Serum ferritin as a measure of stored iron in horses. J Nutr 114, 677-681.
Torti SV, Torti FM. 1998 Human H-kininogen is a ferritin-binding protein. J Biol Chem 273, 13630-13635.
Walters GO, Miller FM, Worwood M. 1973 Serum ferritin concentration and iron stores in normal subjects. J Clin Pathol 26, 770-772.
Watanabe K, Hayashi K, Miyamoto T, Tanaka M, Okano S, Yamamoto S. 2000a Characterization of ferritin and ferritin-binding proteins in canine serum. BioMetals 13, 57-63.
Watanabe K, Muranishi N, Murata Y, Orino K, Okano S, Yamamoto S. 2000b Biochemical properties of canine serum ferritin: iron content and nonbinding to concanavalin A. BioMetals 13, 319-324.
Worwood M, Cragg SJ, Wagstaff M, Jacobs A. 1979 Binding of human serum ferritin to concanavalin A. Clin Sci 56, 83-87.
Worwood M, Cragg SJ, Williams AM, Wagstaff M, Jacobs A. 1982 The clearance of 131I-human plasma ferritin in man. Blood 60, 827-833.
Worwood M, Dawkins S, Wagstaff M, Jacobs A. 1976 The purification and properties of ferritin from human serum. Biochem J 157, 97-103.
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Orino, K., Higa, M., Fujikawa, M. et al. Characterization of autoantibodies to ferritin in canine serum. Biometals 15, 161–165 (2002). https://doi.org/10.1023/A:1015239324414
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DOI: https://doi.org/10.1023/A:1015239324414