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Glucosylation of exogenous vanillin by plant cell cultures

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Abstract

After feeding with vanillin,Vanilla planifolia Andrews cell culture was able to produce the highest amount of glucovanillin compared to Ilex dumosaReissek and Catharanthus roseus (L.) G.Don cell cultures. The optimum yield of glucovanillin was obtained from V. planifolia cells fed with 6.6 mM vanillin and harvested after 12 h. The yield was 3.28 mM glucovanillin (49.7%). This glucoside was stored mainly in the cells.

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References

  • Deus-Neumann B & Zenk MH (1986) Accumulation of alkaloids in plant vacuoles does not involve an ion-trap mechanism. Planta 167: 44–53

    Google Scholar 

  • Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res. 50: 151–158

    Google Scholar 

  • Hallard D, Van der Heijden R, Verpoorte R, Cardoso MIL, Pasquali G, Memelink J & Hoge JHC (1997) Suspension cultured transgenic cells of Nicotiana tabacum expressing tryptophan decarboxylase and strictosidine synthase cDNAs from Catharanthus roseus produce strictosidine upon secologanin feeding. Plant Cell Rep 17: 50–54

    Google Scholar 

  • Kometani T, Tanimoto H, Nishimura T & Okasa S (1993) Glucosylation of vanillin by cultured plant cells. Biosci. Biotech. Biochem. 57(8): 1290–1293

    Google Scholar 

  • Kraemer KH, Schenkel EP & Verpoorte R (1999) Glucosylation of ethanol in Ilex paraguariensis cell suspension cultures. Plant Cell Rep. 18: 509–513

    Google Scholar 

  • Leong G, Uzio R & Derbesy M (1989) Synthesis, identification, and determination of glucosides present in green vanilla beans (Vanilla fragrans Andrews). Flav. Fragr. J. 4: 163–167

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Sommer J, Schroeder C & Stöckigt J (1997) In vivo formation of vanillin glucoside. Plant Cell Tiss. Org. Cult. 50: 119–123

    Google Scholar 

  • Tabata M, Umetani Y, Ooya M & Tanaka S (1988) Glucosylation of phenolic compounds by plant cell cultures. Phytochemistry 27: 809–513

    Google Scholar 

  • Winterhalter P & Skouroumounis GK (1997) Glycoconjugated aroma compounds: Occurence, role and biotechnological transformation. In: Scheper T (ed) Advances in Biochemical Engineering/Biotechnology, Vol 55 (pp 73–105). Springer Verlag, Berlin

    Google Scholar 

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Correspondence to M.J.W. Dignum.

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Yuana, Dignum, M. & Verpoorte, R. Glucosylation of exogenous vanillin by plant cell cultures. Plant Cell, Tissue and Organ Culture 69, 177–182 (2002). https://doi.org/10.1023/A:1015224425075

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  • DOI: https://doi.org/10.1023/A:1015224425075

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