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Plant regeneration from leaf-derived callus in Citrus grandis(pummelo): Effects of auxins in callus induction medium

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Abstract

Calli were induced from leaf explants of seedling in Citrus grandis (L.) Osbeck (pummelo) on MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthaleneacetic acid (NAA), 2,4,5-trichlorophenoxyacetic acid, 2-methyl-4-chlorophenoxyacetic, 4-chlorophenoxyacetic acid, 4-methoxy-3,6-dichlorobenzoic acid or 4-amino-3,5,6-trichloropicolinic acid. 2,4-D was most effective. Only green, compact calli induced by 2,4-D at low concentrations (0.9 and 4.5 μM) were capable of shoot formation and regenerated more than 13 shoots per callus on MS medium containing at least 6.66 μM benzyladenine (BA). Calli induced by other auxins did not regenerate shoots on MS medium containing BA at all concentrations studied. A multiplication rate of 5–7 shoots was achieved from shoot tip culture on MS medium with 0.89 μM BA. Roots developed when regenerated shoots were cultured on MS medium with 9.84 μM indole-3-butyric acid and 5.37 μM NAA. No response was obtained on mature leaves cultured on MS medium supplemented with the above mentioned auxins at various concentrations.

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Authors and Affiliations

  1. South China Institute of Botany, the Chinese Academy of Sciences, Guangzhou, 510650, China

    Huang Tao

Authors
  1. Huang Tao
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  2. Peng Shaolin
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  3. Dong Gaofeng
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  4. Zhang Lanying
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  5. Li Gengguang
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Tao, H., Shaolin, P., Gaofeng, D. et al. Plant regeneration from leaf-derived callus in Citrus grandis(pummelo): Effects of auxins in callus induction medium. Plant Cell, Tissue and Organ Culture 69, 141–146 (2002). https://doi.org/10.1023/A:1015223701161

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  • DOI: https://doi.org/10.1023/A:1015223701161

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