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Effects of Atrial Natriuretic Peptide on DNA Synthesis and NADPH Diaphorase Activity in Epitheliocytes and Smooth Muscle Cells of the Respiratory Tract in Newborn Albino Rats

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Abstract

Newborn rats received single intraperitoneal injections of atrial natriuretic peptide (1-28) in a dose of 3.2×10-8 mol/kg on day 6 of life. Autoradiography with 3H-thymidine showed that the peptide inhibited DNA synthesis in smooth muscle cells of the respiratory tract. Pretreatment with nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester attenuated, but did not abolish the effect of atrial natriuretic peptide (1-28). Histochemical assay for NADPH diaphorase showed that nitric oxide constitutively produced in the epithelium is involved in the growth-inhibitory effect of atrial natriuretic peptide (1-28) on proliferating smooth muscle cells.

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Authors and Affiliations

  1. Institute of Maternity and Child Welfare, Khabarovsk Branch, Far-Eastern Research Center for Physiology and Pathology of Respiration, Siberian Division of the Russian Academy of Medical Sciences; Central Research Laboratory, Far-Eastern State Medical University, Khabarovsk

    O. A. Lebed'ko, S. S. Timoshin & V. I. Tsygankov

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  1. O. A. Lebed'ko
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  2. S. S. Timoshin
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  3. V. I. Tsygankov
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Lebed'ko, O.A., Timoshin, S.S. & Tsygankov, V.I. Effects of Atrial Natriuretic Peptide on DNA Synthesis and NADPH Diaphorase Activity in Epitheliocytes and Smooth Muscle Cells of the Respiratory Tract in Newborn Albino Rats. Bulletin of Experimental Biology and Medicine 133, 26–29 (2002). https://doi.org/10.1023/A:1015187923652

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