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The Fatty Acid Composition of Plasmopara halstedii and Its Taxonomic Significance

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Abstract

The fatty acid composition of sunflower downy mildew, Plasmopara halstedii, was compared to other parasitic Oomycetes and to pathogenic fungi associated with sunflower by gas chromatographic analysis. P. halstedii revealed a characteristic profile differing from all other samples by the dominance of 5Z,8Z,11Z,14Z,17Z-eicosapentaenoic acid. The infraspecific variation between the isolates of sunflower downy mildew was low: no correlation was visible between fatty acid profiles and isolates of different pathotypes or from different geographic origins. Lipids from hyphae, zoosporangia and oospores of the pathogen contained the same fatty acids, though the content of 5Z,8Z,11Z,14Z,17Z-eicosapentaenoic acid was significantly lower in oospores. The taxonomic and diagnostic value of the fatty acid profile in P. halstedii is discussed.

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References

  • Beakes GW (1980) Electron microscopic study of oospore maturation and germination in an emasculate isolate of Saprolegnia ferax. 1. Gross changes. Canadian Journal of Botany 58: 182–194

    Google Scholar 

  • Christie WW (1984) Extraction and hydrolysis of lipids and some reactions of their fatty acid components. In: Mangold HK, Zweig G and Sherma J (eds) CRC Handbook of Chromatography, Lipids, Vol 1 (pp 33–46) CRC Press, Boca Raton

    Google Scholar 

  • Cohen Z, Margheri MC and Tomaselli L (1995) Chemotaxonomy of cyanobacteria. Phytochemistry 40: 1155–1158

    Google Scholar 

  • Cottrell M, Kock JLF, Lategan PM and Britz TJ (1986) Long-chain fatty acid composition as an aid in the classification of the genus Saccharomyces. Systematic and Applied Microbiology 8: 166–168

    Google Scholar 

  • Dick MW (1995) Sexual reproduction in Peronosporomycetes (chromistan fungi). Canadian Journal of Botany 73(suppl. 1): 712–724

    Google Scholar 

  • Erwin JA (1973) Comparative biochemistry of fatty acids in eukaryotic microorganisms. In: Erwin JA (ed) Lipids and Biomembranes of Eukaryotic Microorganisms (pp 41–143) Academic Press, New York

    Google Scholar 

  • Hall GS (1996) Modern approaches to species concepts in downy mildew. Plant Pathology 45: 1009–1026

    Google Scholar 

  • Lösel DM (1988) Fungal lipids. In: Ratledge C and Wilkinson SG (eds) Microbial Lipids, Vol 1 (pp 713–806) Academic Press, London

    Google Scholar 

  • Morrison WR and Smith LM (1964) Preparation of fatty acid methyl esters and dimethyl acetals from lipids with boron fluoride — methanol. Journal of Lipid Research 5: 600–608

    Google Scholar 

  • Nishimura M (1922) Studies in Plasmopara halstedii. Journal of the College of Agricultue, Hokkaido Imperial University 11: 185–210

    Google Scholar 

  • Romano I, Bellitti MR, Nicolaus B, Lama L, Manca MC, Pagnotta E and Gambacorta A (2000) Lipid profile: A useful chemotaxonomic marker for classification of a new cyanobacterium in Spirulina genus. Phytochemistry 54: 289–294

    Google Scholar 

  • Rozynek B and Spring O (2000) Pathotypes of sunflower downy mildew in southern parts of Germany. HELIA 23: 27–34

    Google Scholar 

  • Spring O (2000) Homothallic sexual reproduction in Plasmopara halstedii, the downy mildew of sunflower. HELIA 23: 19–26

    Google Scholar 

  • Spring O and Zipper R (2000) Isolation of oospores of sunflower downy mildew, Plasmopara halstedii, and microscopical studies on oospore germination. Journal of Phytopathology 148: 227–231

    Google Scholar 

  • Tourvieille de Labrouhe D, Gulya TJ, Masirevic S, Penaud A, Rashid KY and Viranyi F (2000) New nomenclature of races of Plasmopara halstedii (sunflower downy mildew). Proceedings of the 15th International Sunflower Conference Toulouse, France, pp i61–i66

  • Van de Peer Y and de Wachter R (1997) Evolutionary relationships among the eukaryotic crown taxa taking into account site-to-site rate variation in 18S-rRNA. Journal of Molecular Evolution 45: 619–630

    Google Scholar 

  • Viljoen BC, Kock JL and Thoupou K (1989) The significance of cellular long-chain fatty acid compositions and other criteria in the study of the relationship between sporogenous ascomycete species and asporogenous Candida species. Systematic and Applied Microbiology 12: 80–90

    Google Scholar 

  • Volkman JK, Dunstan GA, Jeffrey SW and Kearney PS (1991) Fatty acids from microalgae of the genus Pavlova. Phytochemistry 30: 1855–1859

    Google Scholar 

  • Wells J, Civerolo E, Hartung J and Pohronezny K (1993) Cellular fatty acid composition of nine pathovars of Xanthomonas campestris. Journal of Phytopathology 138: 125–136

    Google Scholar 

  • Wood BJB (1988) Lipids of algae and protozoa. In: Ratledge C and Wilkinson SG (eds) Microbial Lipids, Vol 1 (pp 807–867) Academic Press, London

    Google Scholar 

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Authors and Affiliations

  1. Institute of Botany, University of Hohenheim, 70593, Stuttgart, Germany

    Otmar Spring & Klaus Haas

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  1. Otmar Spring
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  2. Klaus Haas
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Spring, O., Haas, K. The Fatty Acid Composition of Plasmopara halstedii and Its Taxonomic Significance. European Journal of Plant Pathology 108, 263–267 (2002). https://doi.org/10.1023/A:1015173900047

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  • DOI: https://doi.org/10.1023/A:1015173900047

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