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Chlorophyll a Fluorescence Response of Norway Spruce Needles to the Long-Term Effect of Elevated CO2 in Relation to Their Position Within the Canopy

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Photosynthetica

Abstract

The long-term impact of elevated CO2 concentration on photosynthetic activity of sun-exposed (E) versus shaded (S) foliage was investigated in a Picea abies stand (age 12 years) after three years of cultivation in adjustable-lamella-domes (ALD). One ALD is supplied with either ambient air [ca. 350 µmol(CO2) mol−1; AC-variant) and the second with elevated CO2 concentration [ambient plus 350 µmol(CO2) mol−1; EC-variant). The pronounced vertical profile of the photosynthetically active radiation (PAR) led to the typical differentiation of the photosynthetic apparatus between the S- and E-needles in the AC-variant estimated from the irradiance-responses of various parameters of the room temperature chlorophyll (Chl) a fluorescence parameters. Namely, electron transport rate (ETR), photochemical efficiency of photosystem 2, PS2 (ΦPS2), irradiance-saturated values of non-photochemical quenching of minimum (SV0) and maximum (NPQ) fluorescence levels, and photochemical fluorescence quenching (qp) at higher irradiances were all significantly higher for E-needles as compared with the S-ones. The prolonged exposure to EC did not cause any stimulation of ETR for the E-needles but a strongly positive effect of EC on ETR was observed for the S-needles resulting in more than doubled ETR capacity in comparison with S-needles from the AC-variant. For the E-needles in EC-variant a slightly steeper reduction of the ΦPS2 and qp occurred with the increasing irradiance as compared to the E-needles of AC-variant. On the contrary, the S-needles in EC variant revealed a significantly greater capacity to maintain a high ΦPS2 at irradiances lower than 200 µmol m−2 s−1 and to prevent the over-reduction of the PS2 reaction centres. Moreover, compared to the AC-variant the relation between SV0 and NPQ exhibited a strong decrease (up to 72 %) of the SV0-NPQ slope for the E-needles and an increase (up to 76 %) of this value for the S-needles. Hence the E- and S-foliage responded differently to the long-term impact of EC. Moreover, this exposure was responsible for the smoothing of the PAR utilisation vertical gradient in PS2 photochemical and non-photochemical reactions within the canopy.

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Authors and Affiliations

  1. Laboratory of Ecological Physiology of Forest Trees, Institute of Landscape Ecology, Academy of Sciences of the Czech Republic, Poříčí 3b, CZ-603 00, Brno, Czech Republic

    M.V. Marek, M. Šprtová & O. Urban

  2. Department of Physics, Faculty of Science, University of Ostrava, Bráfova 7, CZ-701 03, Ostrava 1, Czech Republic

    V. Špunda

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  1. M.V. Marek
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  2. M. Šprtová
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  3. O. Urban
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  4. V. Špunda
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Marek, M., Šprtová, M., Urban, O. et al. Chlorophyll a Fluorescence Response of Norway Spruce Needles to the Long-Term Effect of Elevated CO2 in Relation to Their Position Within the Canopy. Photosynthetica 39, 437–445 (2001). https://doi.org/10.1023/A:1015146830318

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