Skip to main content
SpringerLink
Log in

Temperature and Light Dependence of Photosynthetic Activities in Wheat Seedlings Grown in the Presence of DCMU [3-(3,4-Dichlorophenyl)-1,1-Dimethylurea]

  • Published:
Photosynthetica

Abstract

Photosynthetic electron transfer was studied in thylakoids isolated from control and DCMU-grown wheat (Triticum aestivum L.) seedlings. When exposed to high temperature (HT) and high iradiance (HI), thylakoids showed large variations in the photosynthetic electron transport activities and thylakoid membrane proteins. A drastic reduction in the rate of whole electron transport chain (H2O → MV) was envisaged in control thylakoids when exposed to HT and HI. Such reduction was mainly due to the loss of photosystem 2, PS2 (H2O → DCBQ) activity. The thylakoids isolated from seedlings grown in the presence of DCMU showed greater resistance to HT and HI treatment. The artificial exogenous electron donors MnCl2, DPC, and NH2OH failed to restore the HI induced loss of PS2 activity in both control and DCMU thylakoids. In contrast, addition of DPC and NH2OH significantly restored the HT induced loss of PS2 activity in control thylakoids and partially in DCMU thylakoids. Similar results were obtained when Fv/Fm was evaluated by chlorophyll fluorescence measurements. The marked loss of PS2 activity in control thylakoids was evidently due to the loss of 33, 23, and 17 kDa extrinsic polypeptides and 28-25 kDa LHCP polypeptides.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Akerlund, H.-E., Jansson, C.: Localization of a 34 000 and 23 000 Mr polypeptide to the lumenal side of the thylakoid membrane.-FEBS Lett. 124: 229-232, 1981.

    Google Scholar 

  • Andersson, B., Ljungberg, U., Åkerlund, H.-E., Bishop, N.I.: Discrimination by immunological analysis between two 33-34 kDa polypeptides involved in photosynthetic oxygen evolution.-Biochim. biophys. Acta 809: 288-290, 1985.

    Google Scholar 

  • Armond, P.A., Schreiber, U., Björkman, O.: Photosynthetic acclimation to temperature in the desert shrub, Larrea divaricata. II. Light-harvesting efficiency and electron transport.-Plant Physiol. 61: 411-415, 1978.

    Google Scholar 

  • Armond, P.A., Björkman, O., Staehelin, L.A.: Dissociation of supramolecular complexes in chloroplast membranes. A manifestation of heat damage to the photosynthetic apparatus.-Biochim. biophys. Acta 601: 433-442, 1980.

    Google Scholar 

  • Arnon, D.I.: Copper enzymes in isolated thylakoids. Polyphenoloxidase in Beta vulgaris.-Plant Physiol. 24: 1-15, 1949.

    Google Scholar 

  • Arntz, B., Trebst, B.: On the role of the QB protein of PS II in photoinhibition.-FEBS Lett. 194: 43-49, 1986.

    Google Scholar 

  • Berry, J., Björkman, O.: Photosynthetic response and adaptation to temperature in higher plants.-Annu. Rev. Plant Physiol. 31: 491-543, 1980.

    Google Scholar 

  • Berry, J.A., Fork, D.C., Garrison, S.: Mechanistic studies of thermal damage to leaves.-Carnegie Inst. Year Book 74: 751-759, 1975.

    Google Scholar 

  • Bose, S., Vijayan, P., Santhanam, R., Kandasamy, M.K.: Inhibition of the cation induced reversible changes in excitation energy distribution in thylakoids of BASF 13.338 grown plants.-Biochim. biophys. Acta 1098: 351-358, 1992.

    Google Scholar 

  • Bradbury, M., Baker, N.R.: The kinetics of photoinhibition of the photosynthetic apparatus in pea chloroplasts.-Plant Cell Environ. 9: 286-297, 1986.

    Google Scholar 

  • Cleland, R.E., Critchley, C.: Studies on the mechanism of photoinhibition in higher plants. II. Inactivation by light of photosystem II reaction centre function in isolated spinach chloroplasts and O2 evolving particles.-Photochem. Photobiol. 10: 83-92, 1985.

    Google Scholar 

  • Critchley, C.: The molecular mechanism of photoinhibition — facts and fiction.-Aust. J. Plant Physiol. 15: 27-41, 1988.

    Google Scholar 

  • Eckert, H.-J., Geiken, B., Bernarding, J., Napiwotzki, A., Eichler, H.-J., Renger, G.: Two sites of photoinhibition of the electron transfer in oxygen evolving and Tris-treated PS II membrane fragments from spinach.-Photosynth. Res. 27: 97-108, 1991.

    Google Scholar 

  • Emmett, J.M., Walker, D.A.: Thermal uncoupling in chloroplasts. Inhibition of photophosphorylation without depression of light-induced pH change.-Arch. Biochem. Biophys. 157: 106-113, 1973.

    Google Scholar 

  • Gounaris, K., Brain, A.R.R., Quinn, P.J., Williams, W.P.: Structural reorganisation of chloroplast thylakoid membranes in response to heat stress.-Biochim. biophys. Acta 766: 198-208, 1984.

    Google Scholar 

  • Ivanov, A.G., Velitchkova, M., Kafalieva, D.: Multiple effects of trypsin-and heat-treatments on the ultrastructure and surface charge density of pea chloroplast membranes. Influence on P700+ parameters.-In: Biggins, J. (ed.): Progress in Photosynthesis Research. Vol. 2. Pp. 741-744. Martinus Nijhoff Publ., Dordrecht-Boston-Lancaster 1987.

    Google Scholar 

  • Komenda, J., Masojídek, J.: The effect of Photosystem II inhibitors DCMU and BNT on the high-light induced D1 turnover in two cyanobacterial strains Synechocystis PCC 6803 and Synechocystis PCC 7942.-Photosynth. Res. 57: 193-202, 1998.

    Google Scholar 

  • Komenda, J., Koblížek, M., Prášil, O.: Characterization of processes responsible for the distinct effect of herbicides DCMU and BNT on Photosystem II photoinactivation in cells of the cyanobacterium Synechocystis PCC 7942.-Photosynth. Res. 63: 135-144, 2000.

    Google Scholar 

  • Kulandaivelu, G., Annamalainathan, K.: Interaction of herbicide and ultraviolet-B radiation on the photosynthetic apparatus.-In: Abrol, Y.P., Wattal, P.N., Gnanam, A., Govindjee, Ort, D.R., Teramura, A.H. (ed.): Impact of Global Climatic Changes on Photosynthesis and Plant Productivity. Pp. 59-75. Asi Publ. House, Sittingbourne 1991.

    Google Scholar 

  • Kuwabara, T., Miyao, M., Murata, T., Murata, N.: The function of 33-kDa protein in the photosynthetic oxygen evolution system studied by reconstitution experiments.-Biochim. biophys. Acta 806: 283-289, 1985.

    Google Scholar 

  • Laemmli, U.K.: Clevage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680-685, 1970.

    Google Scholar 

  • Long, S.P., Humphries, S., Falkowski, P.G.: Photoinhibition of photosynthesis in nature.-Annu. Rev. Plant Physiol. Plant mol. Biol. 45: 633-662, 1994.

    Google Scholar 

  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265-275, 1951.

    Google Scholar 

  • Mannan, R.M., Bose, S.: BASF 13.338 induced changes in structure and function of the photosynthetic apparatus in wheat seedlings.-Photochem. Photobiol. 41: 63-72, 1985.

    Google Scholar 

  • Mannan, R.M., Bose, S.: Temperature dependence of photosynthetic activities in wheat seedlings grown in the presence of BASF 13.338 (4-chloro-5-dimethylamino-2-phenyl-3(2H) pyridazinone).-Plant Physiol. 80: 264-268, 1986.

    Google Scholar 

  • Mohanty, N., Murthy, S.D.S., Mohanty, P.: Reversal of heat induced alterations in photochemical activities in wheat přimary leaves.-Photosynth. Res. 14: 259-267, 1987.

    Google Scholar 

  • Nauš, J., Melis, A.: Response of the photosynthetic apparatus in Dunaliella salina to sublethal concentrations of the herbicide 3-(3,4-dichlorophenyl)-1,1-dimethyl urea.-Photosynthetica 26: 67-78, 1992.

    Google Scholar 

  • Nedunchezhian, N., Kulandaivelu, G.: Increased stability of thylakoid components in Vigna sinensis L. seedlings grown under ultraviolet-B enhanced radiation.-Photosynthetica 29: 369-375, 1993.

    Google Scholar 

  • Noorudeen, A.M., Kulandaivelu, G.: On the possible site of inhibition of photosynthetic electron transport by ultraviolet-B (UV-B) radiation.-Physiol. Plant. 55: 161-166, 1982.

    Google Scholar 

  • Ohad, I., Adir, N., Koike, H., Kyle, D.J., Inoue, Y.: Mechanism of photoinhibition in vivo. A reversible light-induced conformational change of reaction centre II is related to an irreversible modification of the D1 protein.-J. biol. Chem. 265: 1972-1874, 1990.

    Google Scholar 

  • Pearcy, R.W., Berry, J.A., Fork, D.C.: Effects of growth tempcrature on thermal stability of the photosynthetic apparatus of Atriplex lentiformis (Torr.) Wats.-Plant Physiol. 59: 873-878, 1977.

    Google Scholar 

  • Powles, S.B.: Photoinhibition on photosynthesis induced by visible light.-Annu. Rev. Plant Physiol. 35: 15-44, 1984.

    Google Scholar 

  • Prášil, O., Adir, N., Ohad, I.: Dynamics of photosystem II: mechanism of photoinhibition and recovery processes.-In: Barber, J. (ed.): The Photosystems: Structure, Function and Molecular Biology. Pp. 295-348. Elsevier Science Publishers, Amsterdam-London-New York-Tokyo 1992.

    Google Scholar 

  • Richter, M., Ruhle, W., Wild, A.: Studies on the mechanism of photosystem II photoinhibition. II. The involvement of toxic oxygen species.-Photosynth. Res. 24: 237-243, 1990.

    Google Scholar 

  • Riethman, H.C., Sherman, L.A.: Purification and characterization of an iron stress-induced chlorophyll-protein from the cyanobacterium Anacystis nidulans.-Biochim. biophys. Acta 935: 141-151, 1988.

    Google Scholar 

  • Roháček, K., Barták, M.: Technique of the modulated chlorophyll fluorescence: basic concepts, useful parameters, and some applications.-Photosynthetica 37: 339-363, 1999.

    Google Scholar 

  • Santarius, K.A.: The protective effect of sugars on chloroplast membranes during temperature and water stress and its relationship to frost, desication and heat resistance.-Planta 113: 105-114, 1973.

    Google Scholar 

  • Seidler, A: Expression of the 23 kDa protein from the oxygen-evolving complex of higher plants in Escherichia coli.-Biochim. biophys. Acta 1187: 73-79, 1994.

    Google Scholar 

  • Siwiak, A., Waloszek, A., Wieckowski, S.: Heat and photosensitivites of chlorophyll-protein complexes in thylakoid membranes isolated from cucumber cotyledons.-Acta Physiol. Plant. 16: 95-100, 1994.

    Google Scholar 

  • Tischer, W., Strotmann, S.: Relationship between inhibitor binding by chloroplasts and inhibition of photosynthetic electron transport.-Biochim. biophys. Acta 460: 113-125, 1977.

    Google Scholar 

  • Waloszek, A., Tulej, M., Wieckowski, S.: Spectroscopic analysis of chlorophyll bleaching in heat pretreated thylakoid membranes isolated from cucumber cotyledons.-Photosynthetica 27: 109-118, 1992.

    Google Scholar 

  • Williams, W.P., Sen, A., Fork, D.C.: Selective photobleaching of PSI-related chlorophylls in heat stressed pea thylakoids.-Photosynth. Res. 10: 75-92, 1986.

    Google Scholar 

  • Wydrzynski, T., Govindjee: A new site of bicabonate effect in photosystem II of photosynthesis. Evidence from chlorophyll fluorescence transients in spinach chloroplasts.-Biochim. biophys. Acta 387: 403-408, 1975.

    Google Scholar 

  • Yordanov, I.: Response of photosynthetic apparatus to temperature stress and molecular mechanisms of its adaptations.-Photosynthetica 26: 517-531, 1992.

    Google Scholar 

  • Zer, H., Ohad, I.: Photoinactivation of photosystem II induces changes in the photochemical reaction centre abolishing the regulatory role of the QB site in the D1 protein degradation.-Eur. J. Biochem. 231: 448-453, 1995.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Energy, Environment and Natural Resources, Madurai Kamaraj University, Madurai, 625 021, India

    K. Muthuchelian

  2. Istituto Agrario di San Michele all' Adige, 38010, San Michele all' Adige, Italy

    M. Bertamini & N. Nedunchezhian

Authors
  1. K. Muthuchelian
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Bertamini
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. Nedunchezhian
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Muthuchelian, K., Bertamini, M. & Nedunchezhian, N. Temperature and Light Dependence of Photosynthetic Activities in Wheat Seedlings Grown in the Presence of DCMU [3-(3,4-Dichlorophenyl)-1,1-Dimethylurea]. Photosynthetica 39, 411–418 (2001). https://doi.org/10.1023/A:1015138628501

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1015138628501

Search

Navigation