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Chlamydospores of Fusarium Oxysporum Schlecht f.sp. orthoceras (Appel & Wollenw.) Bilai as Inoculum for Wheat-flour—Kaolin Granules to be Used for the Biological Control of Orobanche cumana Wallr.

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Abstract

The formation of chlamydospores of Fusarium oxysporum Schlecht. f.sp. orthoceras (Appel & Wollenw.) Bilai, a potential mycoherbicide for the parasitic weed Orobanche cumana Wallr., was optimised regarding the composition of the liquid growth medium. After 16 days of incubation, highest chlamydospore counts (approximately 2 × 107ml−1) were determined in an aqueous solution amended with 1.5% (w/v) finely ground sorghum stover and 30% (v/v) wheat-based stillage, a liquid by-product of ethanol production. The produced fungal biomass retained viability after being air-dried and stored. Granules made from wheat-flour and kaolin (Pesta) containing microconidia or air-dried chlamydospore-rich biomass were prepared and showed high efficacy in controlling O. cumana in the greenhouse. No differences in efficacy were observed between the different types of inoculum used.

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Authors and Affiliations

  1. Institute of Plant Production and Agroecology in the Tropics and Subtropics (380), University of Hohenheim, Garbenstr. 13, D-70593, Stuttgart, Germany

    Dorette Müller-Stöver, Jürgen Kroschel & Joachim Sauerborn

  2. Nisso Chemical Europe GmbH, Steinstraße 27, D-40210, Düsseldorf, Germany

    Heiko Thomas

Authors
  1. Dorette Müller-Stöver
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  2. Jürgen Kroschel
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  3. Heiko Thomas
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  4. Joachim Sauerborn
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Müller-Stöver, D., Kroschel, J., Thomas, H. et al. Chlamydospores of Fusarium Oxysporum Schlecht f.sp. orthoceras (Appel & Wollenw.) Bilai as Inoculum for Wheat-flour—Kaolin Granules to be Used for the Biological Control of Orobanche cumana Wallr.. European Journal of Plant Pathology 108, 221–228 (2002). https://doi.org/10.1023/A:1015104903622

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