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Hemoglobin and subunit multiplicity in the rainbow trout (Oncorhynchus mykiss) hemoglobin system

Fish Physiology and Biochemistry volume 24, pages 335–342 (2001)Cite this article

Abstract

Hemoglobin (Hb) multiplicity is a commonly used index of phylogenetic differentiation and molecular adaptation in fish. Despite improvements in technologies for characterizing protein structure, our knowledge of the component composition of the Hb system in rainbow trout, Oncorhychus mykiss, has remained almost unchanged through three decades. The Hb is considered to consist of four components, of which two (HbI and HbIV) have been extensively characterised with regard to structure and function. Using a variety of molecular techniques we demonstrate the presence of at least nine different Hb fractions composed of nine (five α and four β globin) chains, and that HbI and HbIV consist of two and five individual components, respectively. These findings indicate that the published data on trout HbI and HbIV refer to mixtures of isoHbs and need to be reappraised.

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Authors and Affiliations

  1. Department of Zoophysiology, Institute of Biological Sciences, University of Aarhus, Buiding 131, 8000, Aarhus C, Denmark

    A. Fago & R.E. Weber

  2. Laboratoire de Spectrometrie de Masse des Proteines, Institut de Biologie Structurale, 41 Avenue des Martyrs, 38027, Grenoble Cedex 1, France

    E. Forest

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  1. A. Fago
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  2. E. Forest
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  3. R.E. Weber
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Fago, A., Forest, E. & Weber, R. Hemoglobin and subunit multiplicity in the rainbow trout (Oncorhynchus mykiss) hemoglobin system. Fish Physiology and Biochemistry 24, 335–342 (2001). https://doi.org/10.1023/A:1015063625855

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  • DOI: https://doi.org/10.1023/A:1015063625855

  • fish hemoglobin
  • globin chains
  • mass spectrometry
  • multiplicity
  • root effect
  • trout

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