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Plant Cell, Tissue and Organ Culture

, Volume 69, Issue 1, pp 65–70 | Cite as

Direct somatic embryogenesis from mature embryos of sandalwood

  • V. Ravishankar RaiEmail author
  • Jen McComb
Article

Abstract

Plants were regenerated from mature zygotic embryos of sandalwood (Santalum album L.) through direct somatic embryogenesis. Somatic embryos were formed directly without any intervening callus phase on zygotic embryos plated on Murashige and Skoog (MS) medium containing thidiazuron or benzylaminopurine. Individual somatic embryos were then isolated and transferred to MS medium without cytokinin on which they formed secondary embryos in repetitive cycles with or without the addition of indole acetic acid to the medium. Conversion of somatic embryos into plantlets was achieved by isolating somatic embryos with distinct cotyledons and reculturing them onto half-strength MS medium with GA3 (1.4 μM). Recovered plantlets were acclimatised and grown in the greenhouse. This is the first report on in vitro regeneration via direct somatic embryogenesis of sandalwood.

zygotic embryo repetitive embryogenesis Santalum album

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  1. 1.Department of Studies in Applied Botany and BiotechnologyUniversity of Mysore, ManasagangotriMysoreIndia
  2. 2.Biological SciencesMurdoch UniversityPerthAustralia

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