Abstract
The effects of tissue culture conditions and explant characteristics on direct somatic embryogenesis were studied on Oncidium `Gower Ramsey'. Embryo formation was significantly affected by explant position. Leaf tip segments had a significantly higher embryogenic response than other segments of leaves. Adaxial-side-up orientation significantly promoted embryogenesis in comparison with abaxial-side-up orientation. There was no significant effect of sucrose in a range of concentrations (10–60 g l−1). Modified 1/2-MS medium (containing 85 mg l−1 KH2PO4) supplemented with 170 mg l−1 NaH2PO4 significantly promoted direct somatic embryogenesis. Peptone at 0.5 mg l−1 gave significantly higher emrbyogenic response (80%) on leaf tips than control treatment (50%). The best response on direct embryo formation was obtained on the modified 1/2-MS medium supplemented with 10–20 g l−1 sucrose, 170 mg l−1 NaH2PO4 and 0.5 g l−1 peptone.
Similar content being viewed by others
References
Arditti J & Ernst R (1993) Micropropagation of Orchids. John Wiley and Sons, New York
Chang C & Chang WC (1998) Plant regeneration from callus culture of Cymbidium ensifolium var. misericors. Plant Cell Rep. 17: 251–255
Chen JT, Chang C & Chang WC (1999) Direct somatic embryo-genesis on leaf explants of Oncidium ‘Gower Ramsey’ and subsequent plant regeneration. Plant Cell Rep. 19: 143–149
Chen JT & WC Chang (2000a) Efficient plant regeneration through somatic embryogenesis from callus cultures of Oncidium (Orch-idaceae). Plant Sci. 160: 87–93
Chen JT & WC Chang (2000b) Plant regeneration via embryo and shoot bud formation from flower-stalk explants of Oncidium 'sweet Sugar'. Plant Cell Tiss. Org. Cult. 62: 95–100
Chen JT & Chang WC (2001) Effects of auxins and cytokinins on direct somatic embryogenesis on leaf explants of Oncidium ‘Gower Ramsey’. Plant Growth Regul. (in press)
Dodds JH & Roberts LW(1995) Experiments in plant tissue culture. Cambridge University Press, New York
Duncan DB (1955) Multiple range and multiple F test. Biometrics 11: 1–42
Ishii Y, Takamura T, Goi M & Tanaka M (1998) Callus induction and somatic embryogenesis of Phalaenopsis. Plant Cell Rep. 17: 446–450
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 495–497
Pedroso MC & Pais MS (1995) Factors controlling somatic embryo-genesis. Plant Cell Tiss. Org. Cult. 43: 147–154
Zimmerman JL (1993) Somatic embryogenesis: a model for early development in higher plants. Plant Cell 5: 1411–1423
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chen, JT., Chang, WC. Effects of tissue culture conditions and explant characteristics on direct somatic embryogenesis in Oncidium `Gower Ramsey'. Plant Cell, Tissue and Organ Culture 69, 41–44 (2002). https://doi.org/10.1023/A:1015004912408
Issue Date:
DOI: https://doi.org/10.1023/A:1015004912408