Plant Cell, Tissue and Organ Culture

, Volume 69, Issue 1, pp 41–44 | Cite as

Effects of tissue culture conditions and explant characteristics on direct somatic embryogenesis in Oncidium `Gower Ramsey'

  • Jen-Tsung Chen
  • Wei-Chin ChangEmail author


The effects of tissue culture conditions and explant characteristics on direct somatic embryogenesis were studied on Oncidium `Gower Ramsey'. Embryo formation was significantly affected by explant position. Leaf tip segments had a significantly higher embryogenic response than other segments of leaves. Adaxial-side-up orientation significantly promoted embryogenesis in comparison with abaxial-side-up orientation. There was no significant effect of sucrose in a range of concentrations (10–60 g l−1). Modified 1/2-MS medium (containing 85 mg l−1 KH2PO4) supplemented with 170 mg l−1 NaH2PO4 significantly promoted direct somatic embryogenesis. Peptone at 0.5 mg l−1 gave significantly higher emrbyogenic response (80%) on leaf tips than control treatment (50%). The best response on direct embryo formation was obtained on the modified 1/2-MS medium supplemented with 10–20 g l−1 sucrose, 170 mg l−1 NaH2PO4 and 0.5 g l−1 peptone.

explant orientation explant position medium composition 


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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  1. 1.Institute of BotanyAcademia SinicaTaipeiTaiwan Republic of China

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