Effects of tissue culture conditions and explant characteristics on direct somatic embryogenesis in Oncidium `Gower Ramsey'
- 655 Downloads
The effects of tissue culture conditions and explant characteristics on direct somatic embryogenesis were studied on Oncidium `Gower Ramsey'. Embryo formation was significantly affected by explant position. Leaf tip segments had a significantly higher embryogenic response than other segments of leaves. Adaxial-side-up orientation significantly promoted embryogenesis in comparison with abaxial-side-up orientation. There was no significant effect of sucrose in a range of concentrations (10–60 g l−1). Modified 1/2-MS medium (containing 85 mg l−1 KH2PO4) supplemented with 170 mg l−1 NaH2PO4 significantly promoted direct somatic embryogenesis. Peptone at 0.5 mg l−1 gave significantly higher emrbyogenic response (80%) on leaf tips than control treatment (50%). The best response on direct embryo formation was obtained on the modified 1/2-MS medium supplemented with 10–20 g l−1 sucrose, 170 mg l−1 NaH2PO4 and 0.5 g l−1 peptone.
Unable to display preview. Download preview PDF.
- Arditti J & Ernst R (1993) Micropropagation of Orchids. John Wiley and Sons, New YorkGoogle Scholar
- Chang C & Chang WC (1998) Plant regeneration from callus culture of Cymbidium ensifolium var. misericors. Plant Cell Rep. 17: 251–255Google Scholar
- Chen JT, Chang C & Chang WC (1999) Direct somatic embryo-genesis on leaf explants of Oncidium ‘Gower Ramsey’ and subsequent plant regeneration. Plant Cell Rep. 19: 143–149Google Scholar
- Chen JT & WC Chang (2000a) Efficient plant regeneration through somatic embryogenesis from callus cultures of Oncidium (Orch-idaceae). Plant Sci. 160: 87–93Google Scholar
- Chen JT & WC Chang (2000b) Plant regeneration via embryo and shoot bud formation from flower-stalk explants of Oncidium 'sweet Sugar'. Plant Cell Tiss. Org. Cult. 62: 95–100Google Scholar
- Chen JT & Chang WC (2001) Effects of auxins and cytokinins on direct somatic embryogenesis on leaf explants of Oncidium ‘Gower Ramsey’. Plant Growth Regul. (in press)Google Scholar
- Dodds JH & Roberts LW(1995) Experiments in plant tissue culture. Cambridge University Press, New YorkGoogle Scholar
- Duncan DB (1955) Multiple range and multiple F test. Biometrics 11: 1–42Google Scholar
- Ishii Y, Takamura T, Goi M & Tanaka M (1998) Callus induction and somatic embryogenesis of Phalaenopsis. Plant Cell Rep. 17: 446–450Google Scholar
- Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 495–497Google Scholar
- Pedroso MC & Pais MS (1995) Factors controlling somatic embryo-genesis. Plant Cell Tiss. Org. Cult. 43: 147–154Google Scholar
- Zimmerman JL (1993) Somatic embryogenesis: a model for early development in higher plants. Plant Cell 5: 1411–1423Google Scholar