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Ginkgo biloba Extracts EGb 761 and Bilobalide Increase NADH Dehydrogenase mRNA Level and Mitochondrial Respiratory Control Ratio in PC12 Cells

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Abstract

In the present study, we investigated the effect of Ginkgo biloba extract, EGb 761, and one of its components, bilobalide, on gene expression of subunit 1 of mitochondrial NADH dehydrogenase (ND1) in PC12 cells. By Northern blot analysis we found a ∼2-fold significant increase in ND1 mRNA level, after 48 and 72 h exposure to 100 μg/ml EGb 761 and to 10 μg/ml bilobalide. We also evaluated, by oxygraphy measurements, mitochondrial respiration during state 3 and state 4. In cells treated with EGb 761 and bilobalide for 48 and 72 h, state 4 respiration was significantly decreased, and the respiratory control ratio was increased. These results provide evidence that EGb 761 and bilobalide exert their protective effects by up-regulating mitochondrial ND1 gene expression and by decreasing state 4 respiration, whose increase is thought to be responsible for oxidative damage.

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Authors and Affiliations

  1. Brain Physiology and Metabolism Section, National Institute on Aging, National Institutes of Health, Bethesda, MD, 20892

    Elisabetta A. Tendi, Francesca Bosetti, S. Fenella DasGupta & Stanley I. Rapoport

  2. Section of Biochemistry and Molecular Biology, Department of Chemistry, Faculty of Medicine, University of Catania, 95125, Catania, Italy

    Anna Maria Giuffrida Stella

  3. IHB-IPSEN, 75781, Paris, France

    Katy Drieu

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  1. Elisabetta A. Tendi
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  2. Francesca Bosetti
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  3. S. Fenella DasGupta
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  4. Anna Maria Giuffrida Stella
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  5. Katy Drieu
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  6. Stanley I. Rapoport
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Tendi, E.A., Bosetti, F., DasGupta, S.F. et al. Ginkgo biloba Extracts EGb 761 and Bilobalide Increase NADH Dehydrogenase mRNA Level and Mitochondrial Respiratory Control Ratio in PC12 Cells. Neurochem Res 27, 319–323 (2002). https://doi.org/10.1023/A:1014963313559

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