Skip to main content
Log in

Optically detected magnetic resonance of intact membranes from Chloroflexus aurantiacus. Evidence for exciton interaction between the RC and the B808–866 complex

  • Published:
Photosynthesis Research Aims and scope Submit manuscript

Abstract

Optically detected magnetic resonance of chlorosome-containing membranes from the green filamentous bacterium Chloroflexus aurantiacus has been performed both by fluorescence and absorption detection. Triplet states localized in the chlorosomes and in the B808–866 complex have been characterized. After chemical reduction with ascorbate followed by illumination at 200 K, recombination triplet state localized in the primary donor becomes largely populated under illumination at low temperature while all the antenna triplet states, which are localized in carotenoids and BChl a molecules, are strongly quenched. We were able to obtain the T-S spectrum of the primary donor P870 surrounded by all the antenna complexes connected to the RC via energy transfer and then in its intact environment. We found clear spectroscopic evidence for exciton interaction between the RC and the B808–866 antenna complex. This evidence was provided by the comparison of the T−S spectrum of P870 in the membranes with that of isolated RC. The analogy of some features of the difference spectra with those previously found in the same kind of experiments for Rb. sphaeroides, allows to predict a similar coupling among the primary donor and the nearby antenna BChl a molecules, assembled as circular aggregate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Angerhofer A, Bornhaüser F, Gall A and Cogdell RJ (1995) Optical and optically detected magnetic resonance investigation on purple photosynthetic bacterial antenna complexes. Chem Phys 194: 259–274

    Article  Google Scholar 

  • Brune DC, Nozawa T and Blankenship RE (1987) Antenna organization in green photosynthetic bacteria. Oligomeric bacteriochlorophyll c as a model for the 740 nm absorbing bacteriochlorophyll c in Cf. aurantiacus chlorosomes. Biochemistry 26: 8644–8651

    Article  PubMed  Google Scholar 

  • Brune DC, King GH and Blankenship RE (1988) In: Scheer H and Schneider S (eds) Photosynthetic Light-Harvesting Systems, pp 141–151. Walter de Gruyter, Berlin

    Google Scholar 

  • Carbonera D, Giacometti G and Agostini G (1992a) FDMR of carotenoid and chlorophyll triplets in light harvesting complex LHC II of spinach. Appl Magn Reson 3: 859–872

    Google Scholar 

  • Carbonera D, Giacometti G, Agostini G, Angerhofer A and Aust V (1992b) ODMR of carotenoid and chlorophyll triplets in CP43 and CP47 complexes of spinach. Chem Phys Lett 194: 275–281

    Article  Google Scholar 

  • Carbonera D, Giacometti G and Agostini G (1994) A well resolved triplet minus singlet spectrum of P680 from PS II particles. FEBS Lett 343: 200–204

    Article  PubMed  Google Scholar 

  • Carbonera D, Giacometti G and Segre U (1996) Carotenoid interactions in peridinin chlorophyll a proteins from Dinoflagellates: evidence for optical excitons and triplet migration. J Chem Soc Faraday Trans 92: 989–993

    Article  Google Scholar 

  • Carbonera D, Collareta P and Giacometti G (1997) The P700 triplet state in an intact environment detected by ODMR. A well resolved triplet minus singlet spectrum. Biochim Biophys Acta 1322: 115–128

    Google Scholar 

  • Carbonera D, Bordignon E, Giacometti G, Agostini G, Vianelli A and Vannini C (2001) Fluorescence and absorption detected magnetic resonance of chlorosomes from green bacteria Chlorobium tepidum and Chloroflexus aurantiacus. A comparative study. J Phys Chem B 105: 246–255

    Article  Google Scholar 

  • Fetisova ZG, Kharchenko SG and Abdourakhmanov IA (1986) Strong orientational ordering of the near infrared transition moment vectors of light-harvesting antenna bacterioviridin in chromatophores of the green photosynthetic bacterium Chlorobium limicola. FEBS Lett 199: 234–236

    Article  Google Scholar 

  • Gerola PD and Olson JM (1986) A new bacteriochlorophyll a protein complex associated with chlorosomes of green sulfur bacteria. Biochim Biophys Acta 848: 69–76

    Google Scholar 

  • Griebenow K, Müller MG and Holzwarth AR (1991) Pigment organization and energy transfer in green bacteria. 3. Picosecond energy transfer kinetics within the B808–866 bacteriochlorophyll a antenna complex isolated from Chloroflexus aurantiacus. Biochim Biophys Acta 1059: 226–232

    Google Scholar 

  • Hala J, Searle GFW, Schaafsma TJ, van Hoek A, Pancoska P, Blaha K and Vacek K (1986) Picosecond laser spectroscopy and optically detected magnetic resonance on a model photosynthetic system. Photochem Photobiol 44: 527–534

    Google Scholar 

  • Hayes JM and Small GJ (1986) Photochemical hole burning and strong electron-phonon coupling: primary donor states of reaction centers of photosynthetic bacteria. J Phys Chem 90: 4928

    Article  Google Scholar 

  • Hoff AJ (1989) Optically-detected-magnetic resonance of triplet states. In: Hoff AJ (ed) Advanced EPR. Application in Biology and Biochemistry, pp 633–681. Elsevier Science Publishers, Amsterdam

    Google Scholar 

  • Hoff AJ, Vasmel H, Lous EJ and Amesz J (1988) Triplet minus singlet optical difference spectroscopy of some green photosynthetic bacteria. In: Olson JM, Omerod JG, Amesz J, Stackebrandt E and Trüper HG (eds) Green Photosynthetic Bacteria, pp 119–125. Plenum Press, New York

    Google Scholar 

  • Holzwarth AR and Schaffner K (1994) On the structure of bacteriochlorophyll molecular aggregates in the chlorosomes of green bacteria. A molecular modeling study. Photosynth Res 41: 225–233

    Article  Google Scholar 

  • Matsuura K and Olson JM (1990) Reversible conversion of aggregated bacteriochlorophyll c to the monomeric form by 1-hexanol in chlorosomes from Chlorobium and Chloroflexus. Biochim Biophys Acta 1019: 233–238

    Google Scholar 

  • Matsuura K, Hirota M, Shimada K and Mimuro M (1993) Spectral forms and orientation of bacteriochlorophylls c and a in chlorosomes of the green photosynthetic bacterium Chloroflexus aurantiacus. Photochem Photobiol 57: 92–97

    Google Scholar 

  • Mimuro M, Hirota M, Nishimura Y, Moriyama T, Yamazaki I, Shimada K and Matsuura K (1994) Molecular organization of bacteriochlorophyll in chlorosomes of the green photosynthetic bacterium Chloroflexus aurantiacus: studies of fluorescence depolarization accompanied by energy transfer processes. Photosynth Res 41: 181–191

    Article  Google Scholar 

  • Novoderezhkin V and Fetisova Z (1999) Exciton delocalization in the B808–866 antenna of the green bacterium Chloroflexus aurantiacus as revealed by ultrafast pump-probe spectroscopy. Biophys J 77: 424–430

    PubMed  Google Scholar 

  • Novoderezhkin VI, Taisova AS, Fetisova ZG, Blankenship RE, Savikhin S, Buck DR and Struve WS (1998) Energy transfer in the B808–866 antenna from the green bacterium Chloroflexus aurantiacus. Biophys J 74: 2069–2075

    PubMed  Google Scholar 

  • Olson JM (1998) Chlorophyll organization and Function in Green Photosynthetic bacteria. Photochem Photobiol 67: 61–75

    Article  Google Scholar 

  • Owen GM, Hoff AJ and Jones MR (1997) Excitonic interactions between the reaction center and antennae in purple photosynthetic bacteria. J Phys Chem B 101: 7197–7204

    Article  Google Scholar 

  • Prokhorenko VI, Steensgaard DB and Holzwarth AR (2000) Exciton dynamics in the chlorosomal antennae of the green bacteria Chloroflexus aurantiacus and Chlorobium tepidum. Biophys J 79: 2105–2120

    PubMed  Google Scholar 

  • Sakuragi Y, Frigaard NU, Shimada K and Matsuura K (1999) Association of bacteriochlorophyll a with the CsmA protein in chlorosomes of the photosynthetic green filamentous bacterium Chloroflexus aurantiacus. Biochim Biophys Acta 1413: 172–180

    PubMed  Google Scholar 

  • Scherer POJ and Fischer SF (1987) Model studies to lowtemperature optical transitions of photosynthetic reaction centers. II. Rhodobacter sphaeroides and Chloroflexus aurantiacus. Biochim Biophys Acta 891: 157–164

    Google Scholar 

  • Schmidt K (1980) A comparative study on the composition of chlorosomes (Chlorobium vesicles) and cytoplasmic membranes from Cf. aurantiacus strain Ok-70-fl and Cb. limicola f. thiosulfatophilum strain 6230. Arch Microbiol 124: 21–31

    Article  Google Scholar 

  • Schmidt KA and Trissl HW (1998) Combined fluorescence and photovoltage studies on chlorosomes containing bacteria. Photosynth Res 58: 43–55

    Article  Google Scholar 

  • Sprague SG and Fuller RC (1991) The green phototrophic bacteria and heliobacteria. In: Stolz JF (ed) Structure of Phototrophic Prokaryotes, pp 79–103. CRC Press, Boca Raton, Florida

    Google Scholar 

  • Takaichi S, Tsuji K, Hanada S, Matsuura K and Shimada K (1995) A novel carotenoid glucoside ester in green filamentous bacteria. In: Mathis P (ed) Photosynthesis: From Light to Biosphere, Vol IV, pp 127–130. Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Tsuji K, Takaichi S, Matsuura K and Shimada K (1995) Specificity of carotenoids in chlorosomes of the green filamentous bacterium Cf. aurantiacus. In: Mathis P (ed) Photosynthesis: From Light to Biosphere, Vol IV, pp 99–102. Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • van Amerongen H, Vasmel H and van Grondelle R (1988) Linear dichroism of chlorosomes from Chloroflexus aurantiacus in compressed gel and electric fields. Biophys J 54: 65–76

    Google Scholar 

  • van der Vos R, Carbonera D and Hoff AJ (1991) Microwave and optical spectroscopy of carotenoid triplets in light-harvesting complexes LHC II of spinach by absorbance detected magnetic resonance. Appl Magn Reson 2: 179–202

    Google Scholar 

  • van Dorssen RJ, Vasmel H and Amesz J (1986) Pigment organization and energy transfer in the green photosynthetic bacterium Cf. aurantiacus. II. The chlorosome. Photosynth Res 9: 33–45

    Article  Google Scholar 

  • Vasmel H, van Dorssen RJ, De Vos GJ and Amesz J (1986) Pigment organization and energy transfer in the green photosynthetic bacterium Chloroflexus aurantiacus. I. The cytoplasmic membrane. Photosynth Res 7: 281–294

    Article  Google Scholar 

  • Wullink W, Knudsen J, Olson JM, Redlinger TE and van Bruggen EFJ (1991) Localization of polypeptides in isolated chlorosomes from green phototrophic bacteria by immuno-gold labeling electron microscopy. Biochim Biophys Acta 1060: 97–105

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Donatella Carbonera.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bordignon, E., Scarzello, M., Agostini, G. et al. Optically detected magnetic resonance of intact membranes from Chloroflexus aurantiacus. Evidence for exciton interaction between the RC and the B808–866 complex. Photosynthesis Research 71, 45–57 (2002). https://doi.org/10.1023/A:1014947412940

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1014947412940

Navigation