Skip to main content
SpringerLink
Log in

In vitro mutagenesis induced novel thermo/photoperiod-sensitive genic male sterile indica rice with green-revertible xanthan leaf color marker

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

A series of leaf color mutants were isolated from young inflorescenceculture of thermo/photoperiod-sensitive genic male sterile (T/PGMS) indicarice `Xinguang S'. Most mutants maintained the temperature-insensitivityfor color expression, although some of the mutants weretemperature-sensitive and expressed the mutated colors only under higheror lower temperatures. One elite mutant `R172s' with a single recessivegene controlling green-revertible xanthan marker was found to possess thesame agronomic traits, combining ability, and T/PGMS characteristics as theoriginal parent. The marker-labeled seedlings were easily distinguished fromnormal green seedlings, and their survival rate under field conditions wassignificantly lower than that of hybrid seedlings. These results suggest thatthe leaf color mutations may be used as an ideal marker for monitoring theseed purity in hybrid rice production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bi, CH., Z.B. Li & J.M. Wan, 1990. Effect of the lower temperature in mid-summer on the fertility stability of HPGMR. Chin J Rice Sci 4(4): 181–184.

    Google Scholar 

  • Cao, L., Q. Qian, X. Zhu, D. Zheng, S. Min & Z. Xiong, 1999. Breeding of a photoperiod-sensitive genic male sterile indica rice Zhongsi S with a purple leaf marker and the heterosis of its hybrid rice produced with it. Acta Agron Sinica 25(1): 44–49.

    Google Scholar 

  • Dong, F., X. Zhu & Z. Xiong, 1995. Release of temperaturesensitive genic male sterile M2s with greenish leaf color marker. Chinese Rice Science 9(2): 65–70.

    Google Scholar 

  • Gao, M., Q. Cai & Z. Liang, 1992. In vitro culture of hybrid indica rice combined with mutagenesis. Plant Breeding 108: 104–110.

    Google Scholar 

  • Kato, H., S. Yanwgihara, K. Margama & H. Araki, 1990. Sterility responses of a rice thermo-sensitive genic male in some area of Japan. Japan J Breed 40 (Suppl): 378–379.

    Google Scholar 

  • Li, P., G. Huang, Y. Lin, C. Gao & K. Zhou, 1991. Cultures and fertility expression of ‘T Zhan S’ from rice somaclones. In: Y. Chen (Ed.), Plant Somaclonal Variation and Breeding, pp. 157–160.

  • Jiangsu Science and Technology Publishing House, Nangjing. Maluszynski, M., Beant S., Ahloowalia & Bjorn Siguibjorusson, 1995. Application of in vivo and in vitro mutation techniques for crop improvement. Euphytica 100: 1–13.

    Google Scholar 

  • Oard, J.H., J. Hu & J.N. Rutger, 1991. Genetic analysis of male sterility in rice mutants with environmentally influenced level of fertility. Euphytica: 179–186.

  • Shen, Y. & M. Gao, 1992. Current status on environment-sensitive genic male sterile rice in China. IRRI Newsl 41: 41–46.

    Google Scholar 

  • Shen, Y., M. Gao & Q. Cai, 1994. A novel environment-induced genic male sterile (EGMS) mutant in indica rice. Euphytica 76: 89–96.

    Google Scholar 

  • Shu, Q., Y. Xia & G. Liu, 1995. Development of a colored-leaf mutant line of thermosensitive genic male sterile rice. IRRI Newsl 20(3): 7.

    Google Scholar 

  • Shu, Q., D. Wu & Y. Xia, 1996. Marker-assisted elimination of contaminations in two-line hybrid rice seed production and multiplication. Acta Agriculturae Universitatis Zhejiangenesis 22(1): 56–60.

    Google Scholar 

  • Sing, R. & S.S. Limani, 1990. Recent progress in the technology and development of hybrid rice in Asia. Int Rice Comm Newsl 39: 133.

    Google Scholar 

  • Virmani, S.S., 1990. Prospect and Limitations: the International Conference on seed science and technology, New Dehli, India, pp. 21–25.

  • Wu, D., Y. Xia, Q. Shu & G. Liu, 1997. Induction and identification of temperature-sensitive albino genes in indica rice (Oryza sativa L). Cereal Res Commun 25(4): 905–910.

    Google Scholar 

  • Xia, Y., G. Liu & Q. Shu, 1995. Preliminary analysis of leaf color mutants of temperature-sensitive genic male sterile of indica rice. Acta Agri Nul Sinica 9(3): 129–133.

    Google Scholar 

  • Yuan, L., 1992. The strategy of breeding PGMR and TGMS lines. Hybrid Rice 1: 1–4.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Zhejiang University, Hua-Jia-Chi Campus, Hangzhou, 310029, China

    Dianxing Wu, Qingyao Shu & Yingwu Xia

Authors
  1. Dianxing Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qingyao Shu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yingwu Xia
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wu, D., Shu, Q. & Xia, Y. In vitro mutagenesis induced novel thermo/photoperiod-sensitive genic male sterile indica rice with green-revertible xanthan leaf color marker. Euphytica 123, 195–202 (2002). https://doi.org/10.1023/A:1014924418395

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1014924418395

Search

Navigation