Abstract
Two recombinant plasmids, expressing ice nucleation activity, were constructed and named pCPP30inaZ and pCPP38inaZ. They were transferred to the ice-negative, xanthan-producing Xanthomonas campestris pv. campestris by electroporation. The transformants were used for co-production of xanthan gum and ice nuclei from sugar beet molasses. The highest values obtained were 20 g l−1 and 1018 ice nuclei ml−1, respectively. The above values fulfil the criteria for industrial manipulation. This is the first report on co-formation of two products by a transformed X. campestris strain.
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Afendra, A.S., Yiannaki, E.E., Palaiomylitou, M.A. et al. Co-production of ice nuclei and xanthan gum by transformed Xanthomonas campestris grown in sugar beet molasses. Biotechnology Letters 24, 579–583 (2002). https://doi.org/10.1023/A:1014872811167
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DOI: https://doi.org/10.1023/A:1014872811167