Abstract
Vgt1 (Vegetative to generative transition 1) is a quantitative trait locus (QTL) for flowering time in maize (Zea mays L.). Vgt1 was initially mapped in a ca. 5-cM interval on chromosome bin 8.05, using a set of near-isogenic lines (NILs) in the genetic background of the late dent line N28, with the earliness allele introgressed from the early variety Gaspé Flint. A new large mapping population was produced by crossing N28 and one early NIL with a ca. 6-cM long Gaspé Flint introgression at the Vgt1 region. Using PCR-based assays at markers flanking Vgt1, 69 segmental NILs homozygous for independent crossovers near the QTL were developed. When the NILs were tested in replicated field trials for days to pollen shed (DPS) and plant node number (ND), the QTL followed a Mendelian segregation. Using bulk segregant analysis and AFLP profiling, 17 AFLP markers linked to the QTL region were identified. Statistical analysis indicated a substantial coincidence of the effects of Vgt1 on both DPS and ND. Vgt1 was mapped at ca. 0.3 cM from an AFLP marker. As compared to DPS, the higher heritability of ND allowed for a more accurate assessment of the effects of Vgt1. The feasibility of the positional cloning of Vgt1 is discussed.
Similar content being viewed by others
References
Ahn, S. and Tanksley, S. D. 1993. Comparative linkage maps of the rice and maize genomes. Proc. Natl. Acad. Sci. USA 90: 7980-7984.
Allard, R.W. 1956. Formulas and tables to facilitate the calculation of recombination values in heredity. Hilgardia 24: 235-278.
Alonso-Blanco, C., El-Assal, S.E., Coupland, G. and Koornneef, M. 1998. Analysis of natural allelic variation at flowering time loci in the Landsberg erecta and Cape Verde Islands ecotypes of Arabidopsis thaliana. Genetics 149: 749-764.
Alpert, K.B., Grandillo, S. and Tanksley, S.D., 1995. fw 2.2: amajor QTL controlling fruit weight is common to both red-and greenfruited tomato species. Theor. Appl. Genet. 91: 994-1000.
Araki, T. 2001. Transition from vegetative to reproductive phase. Curr. Opin. Plant Biol. 4: 63-68.
Austin, D.F. and Lee, M. 1996. Genetic resolution and verification of quantitative trait loci for flowering and plant height with recombinant inbred lines of maize. Genome 39: 957-968.
Bassam, B.J., Caetano-Anolles, G. and Gresshoff, P.M. 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal. Biochem. 196: 80-83.
Bickmore, W.A. and Bird, A.P. 1992. Use of restriction endonucleases to detect and isolate genes from mammalian cells. Meth. Enzymol. 216: 224-244.
Brawn, R.I., 1968. Breeding corn for earliness. Proc. Ann. Corn Sorghum Res. Conf. 23: 59-66.
Churchill, G.A and Doerge, R.W. 1994. Empirical threshold values for quantitative trait mapping. Genetics 138: 963-971.
Civardi, L., Xia, Y., Edwards, K.J., Schnable, P.S. and Nikolau, B.J. 1994. The relationship between genetic and physical distances in the cloned a1-sh2 interval of the Zea mays L. genome. Proc. Natl. Acad. Sci. USA 91: 8268-8272.
Cnops, G., de Boer, B., Gerats, A., Van Montagu, M. and Van Lijsebettens M. 1996. Chromosome landing at the Arabidopsis TORNADO1 locus using an AFLP-based strategy. Mol. Gen. Genet. 253: 32-41.
Colasanti, J., Yuan, Z. and Sundaresan, V. 1998. The indeterminate gene encodes a zinc finger protein and regulates a leaf-generated signal required for the transition to flowering in maize. Cell 93: 593-603.
Cross, H.Z. and Zuber, M.S. 1972. Prediction of flowering dates in maize based on different methods of estimating thermal units. Agron. J. 64: 351-355.
Doebley, J., Stec, A. and Gustus, C. 1995. Teosinte branched1 and the origin of maize: evidence for epistasis and the evolution of dominance. Genetics 141: 333-346.
Dooner, H.K., Martinez-Ferez, I.M. 1997. Recombination occurs uniformly within the bronze gene, a meiotic recombination hotspot in the maize genome. Plant Cell 9: 1633-1646.
Frary, A., Nesbitt, T.C., Grandillo, S., Knaap, E., Cong, B., Liu, J., Meller, J., Elber, R., Alpert, K.B. and Tanksley, S.D. 2000. fw2.2: a quantitative trait locus key to the evolution of tomato fruit size. Science 289: 85-88.
Fridman, E., Pleban, T. and Zamir, D. 2000. A recombination hotspot delimits a wild-species quantitative trait locus for tomato sugar content to 484 bp within an invertase gene. Proc. Natl. Acad. Sci. USA 97: 4718-4723.
Gerdes, J.T., Behr, C.F., Coors, J.G. and Tracy, W.F. 1993. Compilation of North American Maize Breeding Germplasm. Crop Science Society of America, Madison, WI.
Graham, G.I., Wolff, S.W. and Stuber, C.W. 1997. Characterization of a yield quantitative trait locus on chromosome five of maize by fine mapping. Crop Sci. 37: 1601-1610.
Haldane, J.B.S. 1919. The combination of linkage values, and the calculation of distance between the loci of linked factors. J. Genet. 8: 299-309.
Hay, R.K.M. and Ellis, R.P. 1998. The control of flowering in wheat and barley: what recent advances in molecular genetics can reveal. Ann. Bot. 82: 541-554.
Jansen, R.C. 1993. Interval mapping of multiple quantitative trait loci. Genetics 135: 205-211.
Jiang, C., Edmeades, G.O., Armstead, I., Lafitte, H.R., Hayward, M.D. and Hoisington, D. 1999. Genetic analysis of adaptation differences between highland and lowland tropical maize using molecular markers. Theor. Appl. Genet. 99: 1106-1119.
Keller, B. and Feuillet, C. 2000. Colinearity and gene density in grass genomes. Trends Plant Sci. 5: 246-251.
Kim, T.S. 1992. Identification of genomic regions controlling maturity in maize (Zea mays L.). Ph.D. Thesis, University of Minnesota, St. Paul, MN.
Konieczny, A. and Ausebel, F.M. 1993. A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. Plant J. 4: 403-410.
Kraja, A.T. and Dudley, J.W. 2000. QTL analysis of two maize inbred line crosses. Maydica 45: 1-12.
Lander, E.S. and Botstein, D. 1989. Mapping mendelian factors underlying quantitative traits using RFLPs linkage maps. Genetics 121:185-199.
Laurie, D.A. 1997. Comparative genetics of flowering time. Plant Mol. Biol. 35: 167-177.
Legare, M.E., Bartlett, F.S. and Frankel, W.N. 2000. A major effect QTL determined by multiple genes in epileptic EL mice. Genome Res. 10: 42-48.
Leng, E.R. 1951. Time relationships in tassel development in inbred and hybrid corn. Agron. J. 43: 445-449.
Lin, Y.-R., Schertz, K. F. and Paterson, A. 1995. Comparative analysis of QTLs affecting plant height and maturity across the Poaceae, in reference to an interspecific sorghum population. Genetics 141: 391-411.
Nei, M. and Lee, W.-H. 1979. Mathematical model for studying genetic variation in terms of restriction endonuclease. Proc. Natl. Acad. Sci. USA 76: 5269-5273.
Neuffer, M.G., Coe, E.H. and Wessler, S.R. 1997. Mutants of maize. Cold Spring Harbor Laboratory Press, Plainview, NY.
Pasyukova, E.G., Vieira, C. and Mackay, T.F.C. 2000. Deficiency mapping of quantitative trait loci affecting longevity in Drosophila melanogaster. Genetics 156: 1129-1146.
Paterson, A.H., De Verna, J.W., Lanini, B. and Tanksley, S.D. 1990. Fine mapping of quantitative trait loci using selected overlapping chromosomes, in an interspecific cross of tomato. Genetics 124: 735-742.
Paterson, A.H., Lander, E.S., Hewitt, J.D., Peterson, S., Lincoln, S.E. and Tanksley, S.D. 1988. Resolution of quantitative traits into Mendelian factors by using a complete RFLP linkage map. Nature 335: 721-726.
Pedersen, C. and Linde-Laursen, I. 1995. The relationship between physical and genetic distance at the Hor1 and Hor2 loci of barley estimated by two colour fluorescent in situ hybridization. Theor. Appl. Genet. 91: 941-946.
Phillips, R.L., Kim, T.S., Kaeppler, S.M., Parentoni, S.N., Shaver, D.L., Stucker, R.I. and Openshaw, S.J. 1992. Genetic dissection of maturity using RFLPs. Proc. Ann. Corn and Sorghum Res. Conf. 47: 135-150.
Robertson, D.S. 1985. A possible technique for isolating genic DNA for quantitative traits in plants. J. Theor. Biol. 117: 1-10.
Russel, W.K. and Stuber, C.W. 1983. Effects of photoperiod and temperatures on the duration of vegetative growth in maize. Crop Sci. 23: 847-850.
Saghai-Maroof, M.A., Soliman, K.M., Jorgesen, R.A. and Allard R.W. 1984. Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc. Natl. Acad. Sci. USA 81: 8014-8018.
Salvi, S., Tuberosa, R. and Phillips, R.L. 2001. Development of PCR-based assays for allelic discrimination in maize, by using the 5'-nuclease procedure. Mol. Breed, 8: 169-176.
Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Plainview, NY.
Simons, G., van der Lee, T., Diergaarde, P., van Daelen, R., Groenendijk, J., Frijters, A., Buschges, R., Hollricher, K., Topsch, S., Schulze-Lefert, P., Salamini, F., Zabeau, M. and Vos, P. 1997. AFLP-based fine mapping of the Mlo gene to a 30-kb DNA segment of the barley genome. Genomics 44: 61-70.
Simpson, GG., Gendall, A.R. and Dean, C. 1999. When to switch to flowering. Ann. Rev. Cell. Dev. Biol. 99: 519-550.
Sommer, S.S., Groszbach, A.R. and Bottema, C.D.K. 1992. PCR amplification of specific alleles (PASA) is a general method for rapidly detecting known single-base changes. Biotechniques 12: 82-87.
Stein, N., Feuillet, C., Wicker, T., Schlagenhauf, E. and Keller, B. 2000. Subgenome chromosome walking in wheat: a 450-kb physical contig in Triticum monococcum L. spans the Lr10 resistance locus in hexaploid wheat (Triticum aestivum L.) Proc. Natl. Acad. Sci. USA. 97: 13436-13441.
Tanksley, S.D., Ganal, M.W. and Martin, G.B. 1995. Chromosome landing: a paradigm for map-based gene cloning in plants with large genomes. Trends Genet. 11: 63-68.
Thoday, J.M. 1961. Location of polygenes. Nature 191: 368-370.
Tikhonov, A.P., SanMiguel P.J., Nakajima, Y., Gorenstein, N.M., Bennetzen, J.L. and Avramova, Z. 1999. Colinearity and its exceptions in orthologous ADH regions of maize and sorghum. Proc. Natl. Acad. Sci. USA. 96: 7409-7414.
Timmermans, M.C., Das, O.P. and Messing, J. 1996. Characterization of a meiotic crossover in maize identified by a restriction fragment length polymorphism-based method. Genetics 143: 1771-1783.
Tuberosa, R., Salvi, S., Sanguineti, M.C., Landi, P., Conti, S., Frascaroli, E. and Noli, E. 1997. Identification of QTLs for leaf abscisic acid concentration and agronomic traits in droughtstressed maize. Plant and Animal Genome Conference, San Diego, USA, W5.
Vladutu, C., McLaughlin J. and Phillips R.L. 1999. Fine mapping and characterization of linked quantitative trait loci involved in the transition of the maize apical meristem from vegetative to generative structures. Genetics 153: 993-1007.
Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M. and Zabeau, M. 1995. AFLP: a new technique for DNA fingerprinting. Nucl. Acids Res. 11: 4407-4414.
Wang, R.-L., Stec, A., Hey, J., Lukens, L. and Doebley, J. 1999. The limits of selection during maize domestication. Nature 398: 236-239.
Wehrhahn, C. and Allard, R.W., 1965. The detection and measurement of the effects of individual genes involved in the inheritance of a quantitative trait in wheat. Genetics 51: 109-119.
Wei, F., Gobelman-Werner, K., Morroll, S.M., Kurth, J., Mao, L., Wing, R., Leister, D., Schulze-Lefert, P. and Wise, R.P. 1999. TheMla (powdery mildew) resistance cluster is associated with three NBS-LRR gene families and suppressed recombination within a 240-kb DNA interval on chromosome 5S (1HS) of barley. Genetics 153: 1929-1948.
Yamamoto, T., Kuboki, Y., Lin, S.Y., Sasaki, T. and Yano, M. 1998. Fine mapping of quantitative trait loci, Hd-1, Hd-2 and Hd-3, controlling heading date of rice as single Mendelian factors. Theor. Appl. Genet. 97: 37-44.
Yano, M., Katayose, Y., Ashikari, M., Yamanouchi, U., Monna, L., Fuse, T., Baba, T., Yamamoto, K., Umehara, Y., Nagamura, Y. and Sasaki, T. 2000. Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell 12: 2473-2484.
Yu, X.G., Bush, A.L. and Wise, R. 1996. Comparative mapping of homoeologous group 1 regions and genes for resistance to obligate biotrophs in Avena, Hordeum, and Zea mays. Genome 39: 155-164.
Zamir D. and Eshed, Y. 1998. In: A.H. Paterson (Ed.) Molecular Dissection of Complex Traits, CRC, Boca Raton, FL, pp. 207-217.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Salvi, S., Tuberosa, R., Chiapparino, E. et al. Toward positional cloning of Vgt1, a QTL controlling the transition from the vegetative to the reproductive phase in maize. Plant Mol Biol 48, 601–613 (2002). https://doi.org/10.1023/A:1014838024509
Issue Date:
DOI: https://doi.org/10.1023/A:1014838024509