Abstract
Zanthoxylum piperitum (prickly ash) was grown as a suspension culture in Schenk and Hildebrandt medium supplemented with 50 g sucrose l−1 and 0.5 mg 2,4-dichlorophenoxyacetic acid l−1 for 21 days with elicitation by pectin added at day 15. Volatile compounds were extracted from the culture and 3-hydroxy-2-butanone was identified by GC-MS as the most abundant compound, followed by γ-butyrolactone and 2,3-butanedione. 2,3-Butanedione, ethyl 3-methylbutyrate, and ethyl 2-methylpropanoate were identified as the most intense aroma-active compounds and represented the characteristic aroma of the culture.
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References
Chang JH, Shin JH, Chung IS, Lee HJ (1998) Improved menthol production from chitosan-elicited suspension culture of Mentha piperita. Biotechnol. Lett. 20: 1097-1099.
Collin HA (1988) Flavors. In: Constabel F, Vasil IK, eds. Cell Culture and Somatic Cell Genetics of Plants, Vol. 5. San Diego, CA: Academic Press, pp. 569-585.
Dönenburg H, Knorr D (1995) Strategies for the improvement of secondary metabolite production in plant cell cultures. Enzyme Microb. Technol. 17: 674-684.
Fenn PF, Durbin RD, Kuntz JE (1977) Synthesis of tryptophol and o-acetyltryptophol from tryptophan by Ceratocystis fagacearum. Phytochemistry 16: 899-901.
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151-158.
Kim JH, Lee KS, Oh WT, Kim KR (1989) Flavor components of the fruit peel and leaf oil from Zanthoxylum piperitum DC. Korean J. Food Sci. Technol. 21: 562-568.
Kojima H, Kato A, Kubota K, Kobayashi A (1997) Aroma compounds in the leaves of Japanese pepper (Zanthoxylum piperitum DC) and their formation from glycosides. Biosci. Biotechnol. Biochem. 61: 491-494.
Lin ML, Staba EJ (1961) Peppermint and spearmint tissue cultures. I. Callus formation and submerged culture. Lloydia 24: 139-145.
Mills DR, Lee JM (1996) A simple, accurate method for determining wet and dry weight concentration of plant cell suspension cultures using microcentrifuge tubes. Plant Cell Rep. 15: 634-636.
Mistry BS, Reineccius T, Olson LK (1997) Gas chromatographyolfactometry for the determination of key odorants in foods. In: Marsili R, ed. Techniques for Analyzing Food Aroma. New York: Marcel Dekker, pp. 265-292.
Molimard P, Spinnler HE (1996) Compounds involved in the flavor of surface mold-ripened cheeses: origins and properties. J. Dairy Sci. 79: 169-184.
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant 15: 473-497.
Murashige T, Tucker DPH (1969) Growth factor requirements of citrus tissue culture. In: Chapman HD, ed. Proceedings of the 1st International Citrus Symposium, Vol. 3. University of California, Reverside: Reverside Publication, pp. 1155-1161.
Park IS, Kim DI (1993) Significance of fresh weight to dry cell weight ratio in plant cell suspension cultures. Biotechnol. Tech. 7: 627-630.
Sahai O (1994) Plant tissue culture. In: Gabelman A, ed. Bioprocess Production of Flavor, Fragrance, and Color Ingredients. New York: John Wiley & Sons, pp. 239-275.
Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199-204.
Schieberle P, Hofmann T (1997) Evaluation of the character impact odorants in fresh strawberry juice by quantitative measurements and sensory studies on model mixtures. J. Agric. Food Chem. 45: 227-232.
Song WS, Oh SD, Park EH, Yu SO (1991) In vitro propagation of Zanthoxylum piperitum DC. I. Somatic embryogenesis and plant regeneration. Korean J. Plant Tiss. Cult. 18: 17-25.
Speranza G, Corti S, Fontana G, Manitto P (1997) Conversion of meso-2,3-butanediol into 2-butanol by lactobacilli. Stereochemical and enzymatic aspects. J. Agric. Food Chem. 45: 3476-3480.
Van den Dool H, Kratz PD (1963) A generalisation of the retention index system including linear temperature programmed gas-liquid partition chromatography. J. Chromatogr. 11: 463-471.
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Kim, T.H., Kim, T.H., Shin, J.H. et al. Characteristics of aroma-active compounds in the pectin-elicited suspension culture of Zanthoxylum piperitum (prickly ash). Biotechnology Letters 24, 551–556 (2002). https://doi.org/10.1023/A:1014812508441
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DOI: https://doi.org/10.1023/A:1014812508441