Abstract
A cell density-dependent metabolic switch in amino acid metabolism occurs in E. coli W3110 batch cultures at 1.15 g dry wt l−1 (Han L, Doverskog M, Enfors S-O, Häggström L, 2002, J. Biotechnol. 92: 237–249). A two- to three-fold decrease of the concentration of most glycolytic and citric acid cycle metabolites, and an increase in acetyl-CoA concentration after the switch, indicates that the central metabolism also is affected. The specific acetate production rate decreases throughout the culture, except for a temporary increase at the switch point. The intracellular acetate concentration remains relatively constant during the culture.
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Han, L., Enfors, SO. & Häggström, L. Changes in intracellular metabolite pools, and acetate formation in Escherichia coli are associated with a cell-density-dependent metabolic switch. Biotechnology Letters 24, 483–488 (2002). https://doi.org/10.1023/A:1014586205347
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DOI: https://doi.org/10.1023/A:1014586205347