Abstract
Comparison of the putative iteron-binding proteins of lambdoid phages allows us to propose that in the case of lambdoid replication modules, the units on which natural selection acts do not coincide with the open reading frames. Rather, the first replication gene is split into two segments, and its 3′ part (corresponding to the C-terminal domain of the iteron-binding protein) forms one unit with the second gene. We also propose from the phylogenetic analysis of phage-encoded homologs of E. coli DnaB and DnaC, that the recombination with the host sequences is not frequent. Accessory ATP-ases for helicase loading (E. coli DnaC homologs) may not be universal replication proteins. Our analysis may suggest that the bacterial helicase loaders might be of phage origin. The comparison of DnaC homologs of enterobacteria and enterobacterial phages supports the experimental data on residues important in interaction with DnaB. We propose that construction of plasmids carrying the replication origins of lambdoid prophages could be useful not only in further research on DNA replication but also on the role of these prophages in shuttling genes for bacterial virulence. The phage replication sequences could be also useful for identification of clinical enterobacterial isolates.
Similar content being viewed by others
![](https://media.springernature.com/w215h120/springer-static/image/art%3A10.1007%2Fs11262-020-01734-8/MediaObjects/11262_2020_1734_Fig1_HTML.png)
References
Hendrix R.W., Smith M.C., Burns R.N., Ford M.E., and Hatfull G.F., Proc Natl Acad Sci USA 96, 2192-2197, 1999.
Brussow H. and Desiere F., Mol Microbiol 39, 213-222, 2001.
Hatfull G.F. and Sarkis G.J., Mol Microbiol 7, 395-405, 1993.
Campbell A., Annu Rev Micriobiol 48, 193-222, 1994.
Bergh O., Borsheim Y., Bratbak G., and Heldal M., Nature 340, 467-468, 1989.
Anilionis A. and Riley M., J Bacteriol 143, 355-365, 1980.
Diaz R. and Pritchard R.H., Nature 275, 561-564, 1978.
Diaz R., Barnsley P., and Pritchard R.H., Mol Gen Genet 175, 151-157, 1979.
Taylor K. and Węgrzyn G., FEMS Microbiol Rev 17, 109-119, 1995.
Moore D.D., Denniston K.J., and Blattner F.R., Gene 14, 91-101, 1981.
Odegrip R., Shoen S., Haggard-Ljunngquist E., Park K., and Chattoraj D.K., J Virol 74, 4057-4063, 2000.
Smith T.F. and Waterman M.S., J Mol Biol 147, 195-197, 1981.
Thompson J.D., Higgins D.G., and Gibson T.J., Nucleic Acid Res 22, 4673-4680, 1994.
Strimmer K. and von Haeseler A., Mol Biol Evol 13, 964-969, 1996.
Potrykus K., Wróbel B., Węgrzyn A. and Węgrzyn G., Plasmid 44, 111-126, 2000.
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., and Shao Y., Science 277, 1453-1474, 1997.
Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W., Lim A., Dimalanta E., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., and Blattner F.R., Nature 409, 529-533, 2001.
Imai Y., Ogosawara N., Ishigo-oka D., Kadoya R., Daito T., and Moriya S., Mol Microbiol 36, 1037-1048, 2001.
Koonin E.V., Nucleic Acid Res 20, 1997, 1992.
Ludlam A.V., McNatt M.W., Carr K.M., and Kaguni J.M., J Biol Chem 276, 27345-27353
Nakayama N., Bond M.W., Miyajima A., Kobori J., and Arai K., J Biol Chem 262, 10475-10480, 1987.
Dodd I.B. and Egan J.B., Nucleic Acids Res 18, 5019-5026, 1990.
Simpson A.J.G. et al., Nature 406, 151-157, 2000.
Backhaus H. and Petri J.B., Gene 32, 289-303, 1984.
Desiere F., Pridmore R.D., and Brussow H., Virology 275, 294-305, 2000.
Caspi R., Pacek M., Consiglieri G., Helinski D.R., Toukdarian A., and Konieczny I., EMBO J 20, 3262-3271, 2001.
Mallory J.B., Alfano C., and McMacken R., J Biol Chem 265, 13297-13307, 1990.
Konieczny I. and Marszalek J., J Biol Chem 270, 9792-9799, 1995.
Moran N.A., Munson M.A., Baumann P., and Ishikawa H., Proc R Soc Lond Biol Sci 253, 167-171.
Figueroa-Bossi N.F., Coissac E., Netter P., and Bossi L., Mol Microbiol 25, 161-173, 1997.
Figueroa-Bossi N.F. and Bossi L., Mol Microbiol 28, 1040-1041, 1998.
Figueroa-Bossi N.F. and Bossi L., Mol Microbiol 33, 167-176, 1999.
Figueroa-Bossi N.F., Uzzau S., Maloriol D., and Bossi L., Mol Microbiol 39, 260-271, 2001.
Diaz R. and Kaiser K., Mol Gen Genet 183, 483-489, 1981.
Bakshi C.S., Singh V.P., Wood M.W., Jones P.W., Wallis T.S., and Galyov E.E., J Bacteriol 182, 2341-2344, 2000.
Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P., Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M., Rutherford K., Simmonds M., Skelton J., Stevens K., Whitehead S., and Barrell B.G., Nature 413, 848-852, 2001.
Miao E.A. and Miller S.I., Proc Natl Acad Sci USA 96, 9452-9454, 1999.
Pritchett L.C., Konkel M.E., Gay J.M., and Besser T.E., J Clin Microbiol 38, 3484-3488, 2000.
Węgrzyn A., Węgrzyn G., Herman A., and Taylor K., Genes Cells 1, 953-963, 1996.
Węgrzyn G. and Taylor K., J Mol Biol 226, 681-688, 1992.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wróbel, B., Węgrzyn, G. Evolution of Lambdoid Replication Modules. Virus Genes 24, 163–171 (2002). https://doi.org/10.1023/A:1014576701341
Issue Date:
DOI: https://doi.org/10.1023/A:1014576701341