Skip to main content
Log in

Characterization of resistance genes against scald (Rhynchosporium secalis (Oudem.) J.J. Davis) in barley (Hordeum vulgare L.) lines from central Norway, by means of genetic markers and pathotype tests

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Rhynchosporium secalis is a serious pathogen of barley (Hordeum vulgare L.) in central Norway. A breeding effort was initiated in 1977 to introduce resistance from different sources into adapted genotypes, and the first cultivar from the program was recently released. However, little is known about the resistance genes introgressed in this cultivar or in advanced breeding lines. An effort was made to address this issue through a set of isolates and available molecular markers. Fourteen breeding lines and their resistance donors were investigated by evaluating their reactions to 11 R. secalis isolates. Bulked segregant analysis was used to identify molecular markers linked to resistance genes in 12 of the breeding lines. The isolates were found to be of less discriminating value than the markers. Useful information has been obtained as to the nature of several of the resistance genes introgressed. Eight of the 12 breeding lines contained introgressed genes that were located at the `complex Rh' locus on chromosome 3H and hence may not easily be pyramided into the same genotype. Previous information about the nature of the resistance in `Jet' is questioned. Neither of the resistance genes Rh or Rh2 seems to have been incorporated into Norwegian breeding material.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Abbott, D.C., A.H.D. Brown & J.J. Burdon, 1992. Genes for scald resistance from wild barley (Hordeum vulgare ssp. spontaneum) and their linkage to isozyme markers. Euphytica 61: 225–231.

    Google Scholar 

  • Abbott, D.C., E.S. Lagudah & A.H.D. Brown, 1995. Identification of RFLPs flanking a scald resistance gene on barley chromosome 6. J Heredity 86: 152–154.

    Google Scholar 

  • Baker, R.J. & E.N. Larter, 1963. The inheritance of scald resistance in barley. Can J Genet Cytol 5: 445–449.

    Google Scholar 

  • Barua, U.M., K.J. Chalmers, C.A. Hackett, W.T.B. Thomas, W. Powell & R. Waugh, 1993. Identification of RAPD markers linked to a Rhynchosporium secalis resistance locus in barley using near-isogenic lines and bulked segregant analysis. Heredity 71: 177–184.

    Google Scholar 

  • Beer, W.W., 1991. Leaf blotch of barley (Rhynchosporium secalis). Zentralbl Mikrobiol 146: 339–358.

    Google Scholar 

  • Bockelman, H.E., E.L. Sharp & R.F. Eslick 1977. Trisomic analysis of genes for resistance to scald and net blotch in several barley cultivars. Can J Bot 55: 2142–2148.

    Google Scholar 

  • Ceoloni, C., 1980. Race differentiation and search for sources of resistance to Rhynchosporium secalis in barley in Italy. Euphytica 29: 547–553.

    Google Scholar 

  • Dyck, P.L. & C.W. Schaller, 1961a. Association of two genes for scald resistance with a specific barley chromosome. Can J Genet Cytol 3: 165–169.

    Google Scholar 

  • Dyck, P.L. & C.W. Schaller, 1961b. Inheritance of resistance in barley to several physiologic races of the scald fungus. Can J Genet Cytol 3: 153–164.

    Google Scholar 

  • Elen, I., 1987. Parallel development of scald virulence and distribution of varietal resistance in barley. Växtskyddsrapporter: Jordbruk 48: 18–20.

    Google Scholar 

  • Garvin, D.F., A.H.D. Brown & J.J. Burdon, 1997. Inheritance and chromosome locations of scald-resistance genes derived from Iranian and Turkish wild barleys. Theor Appl Genet 94: 1086–1091.

    Google Scholar 

  • Garvin, D.F., A.H.D. Brown, H. Raman & B.J. Read, 2000. Genetic mapping of the barley Rrs14 scald resistance gene with RFLP, isozyme and seed storage protein markers. Plant Breeding 119: 193–196.

    Google Scholar 

  • Goodwin, S.B., R.W. Allard & R.K. Webster, 1990. A nomenclature for Rhynchosporium secalis pathotypes. Phytopathology 80: 1330–1336.

    Google Scholar 

  • Graner, A., A. Jahoor, J. Schondelmaier, H. Siedler, K. Pillen, G. Fischbeck, G. Wenzel & R.G. Herrmann, 1991. Construction of an RFLP map of barley. Theor Appl Genet 83: 250–256.

    Google Scholar 

  • Graner, A. & A. Tekauz, 1996. RFLP mapping in barley of a dominant gene conferring resistance to scald (Rhynchosporium secalis). Theor Appl Genet 93: 421–425.

    Google Scholar 

  • Grønnerød, S., A.G. Maroy, J. MacKey, A. Tekauz, G.A. Penner & A. Bjørnstad, 2001a. Genetic analysis of resistance to barley scald (Rhynchosporium secalis). I. The Ethiopian line ‘Abyssinian' (Submitted to Euphytica).

  • Grønnerød, S., A.G. Maroy, J. MacKey, A. Tekauz & A. Bjørnstad, 2001b. Genetic analysis of resistance to barley scald (Rhynchosporium secalis). II. The Ethiopian donor lines ‘steudelli’ and ‘Jet’, in prep.

  • Habgood, R.M. & J.D. Hayes, 1971. The inheritance of resistance to Rhynchosporium secalis in barley. Heredity 27: 25–37.

    Google Scholar 

  • Hansen, L.R. & H.A. Magnus, 1973. Virulence spectrum of Rhynchosporium secalis in Norway and sources of resistance in Barley. Phytopathol Zeitschr 76: 303–313.

    Google Scholar 

  • Heun, M., A.E. Kennedy, J.A. Anderson, N.L.V. Lapitan, M.E. Sorrells & S.D. Tanksley, 1991. Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare). Genome 34: 437–447.

    Google Scholar 

  • Kleinhofs, A., A. Killian, M.A. Saghai Maroof, R.M. Biyashev, P. Hayes, F.Q. Chen, N. Lapitan, A. Fenwick, T.K. Blake, V. Kanazin, E. Ananiev, L. Dahleen, D. Kudrna, J. Bollinger, S.J. Knapp, B. Liu, M. Sorrells, M. Heun, J.D. Franckowiak, D. Hoffman, R. Skadsen & B.J. Steffenson, 1993. A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theor Appl Genet 86: 705–712.

    Google Scholar 

  • Lyngs Jørgensen, H.J., 1992. Rhynchosporium secalis, Effect on Yield, Physiologic Specialization and Infection Biology. The Royal Vet and Agric Univ, Copenhagen, Denmark. Ph.D. Thesis. 136 pp.

    Google Scholar 

  • Mackie, W.W., 1929. Inheritance of resistance to barley scald. Phytophathology 19: 1141.

    Google Scholar 

  • Michelmore, R.W., I. Paran & R.V. Kesseli, 1991. Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88: 9828–9832.

    Google Scholar 

  • Newton, A.C., 1997. Cultivar mixtures in intensive agriculture. In: I.R. Crute, E.B. Holub & J.J. Burdon (Eds.), The Genefor-Gene Relationship in Plant-Parasite Interactions, pp. 65–80, CAB International.

  • Paltridge, N.G., N.C. Collins, A. Bendahmane & R.H. Symons, 1998. Development of YLM, a codominant PCR marker closely linked to the Yd2 gene for resistance to barley yellow dwarf disease. Theor Appl Genet 96: 1170–1177.

    Google Scholar 

  • Penner, G.A., A. Tekauz, E. Reimer, G.J. Scoles, B.G. Rossnagel, P.E. Eckstein, W.G. Legge, P.A. Burnett, T. Ferguson & J.F. Helm, 1996. The genetic basis of scald resistance in western Canadian barley cultivars. Euphytica 92: 367–374.

    Google Scholar 

  • Ramsay, L., M. Macaulay, K. McLean, J. Fuller, K. Edwards, S. Tuvesson, M. Morgante, S. degli Ivanissivich, N. Marmiroli, E. Maesti, A. Massari, W. Powell & R. Waugh, 2000. A simple sequence repeat – based linkage map of barley. Genetics 156 (in press).

  • Reitan, L., 1997. Resistens mot grå øyeflekk hos bygglinjer i Midt-Norge. Jord-og plantekultur 04/97, forsøksresultater 1996. Grønn forskning, Planteforsk Apelsvoll. 220 pp.

    Google Scholar 

  • Salamati, S., 1997. Barley leaf blotch (Rhynchosporium secalis): on pathogenic variability, epidemiology and control. Agricultural University of Norway, Ph.D. Thesis.

  • Salamati, S. & A.M. Tronsmo, 1997. Pathogenicity of Rhynchosporium secalis isolates from Norway on 30 cultivars of Barley. Plant Pathology 46: 416–424.

    Google Scholar 

  • Salamati, S., J. Zhan, J.J Burdon & B.A. McDonald, 2000. The genetic structure of field populations of Rhynchosporium secalis from three continents suggests moderate gene flow and regular recombination. Phytopathology 90: 901–908.

    Google Scholar 

  • Schweizer, G.F., M. Baumer, G. Daniel, H. Rugel & M.S. Röder, 1995. RFLP markers linked to scald (Rhynchosporium secalis) resistance gene Rh2 in barley. Theor Appl Genet 90: 920–924.

    Google Scholar 

  • Wells, S.A. & W.P. Skoropad, 1963. Inheritance of reaction to Rhynchosporium secalis in barley. Can J Plant Sci 43: 184–187.

    Google Scholar 

  • Williams, R.J. & H. Owen, 1973. Physiologic races of Rhynchosporium secalis on barley in Britain. Trans Br Mycol Soc 60: 223–234.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Reitan, L., Grønnerød, S., Ristad, T. et al. Characterization of resistance genes against scald (Rhynchosporium secalis (Oudem.) J.J. Davis) in barley (Hordeum vulgare L.) lines from central Norway, by means of genetic markers and pathotype tests. Euphytica 123, 31–39 (2002). https://doi.org/10.1023/A:1014442218346

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1014442218346

Navigation