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Serum IgG2 Concentration Is Associated with Gm-Allotypes of IgG2 But Not with the R131H Polymorphism of Human Fcγ Receptor Type IIa

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Abstract

Serum concentrations of immunoglobulins IgG, IgG1, and IgG2 were determined in 62 Finnish subjects who were also typed for Gm(n) allele of IgG2 and R131 and H131 alleles of the Fcγ receptor IIa. Statistically significant G2m-allotype-associated differences in serum concentrations of IgG2 were found; the mean concentration of IgG2 was high in Gm(n)-positive homozygotes (3.9 g/liter) and low in Gm(n)-negative individuals (2.6 g/liter; P = 0.0036), which is in accordance with previous reports. Contrary to an earlier report, no statistically significant R131/H131-allotype-associated differences were found in serum concentrations of IgG2, not even in the case where the IgG2 concentration was calculated relative to the IgG1 or IgG concentration (IgG2/IgG1 or IgG2/IgG). The gene frequencies of R131 and H131 alleles were 0.516 and 0.484, respectively, which did not differ significantly from those reported earlier for Finnish or other Caucasian populations.

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REFERENCES

  1. Seppälä IJT, Sarvas H, Mäkelä O: Low concentrations of Gm allotypic subsets G3 m(g) and G1(f) in homozygotes and heterozygotes. J Immunol 151:2529–2537, 1993

    Google Scholar 

  2. Normansell DE: Human immunoglobulin subclasses. Diagn Clin Immunol 5:115–128, 1987

    Google Scholar 

  3. Johansen FE, Braathen R, Brandtzaeg P: Role of J chain in secretory immunoglobulin formation. Scand J Immunol 52(3): 240–248, 2000

    Google Scholar 

  4. Ambrosino DM, Schiffman G, Gotschlich EC, Schur PH, Rosenberg GA, DeLange GG, van Loghem E, Siber GR: Correlation between G2m(n) immunoglobulin allotype and human antibody response and susceptibility to polysaccharide encapsulated bacteria. J Clin Invest 75:1935–1942, 1985

    Google Scholar 

  5. Sarvas H, Rautonen N, Mäkelä O: Allotype-associated differences in concentrations of human IgG subclasses. J Clin Immunol 11:39–45, 1991

    Google Scholar 

  6. Parren PWHI, Warmerdam PAM, Boeije LCM, Arts J, Westerdaal NAC, Vlug A, Capel PJA, Aarden LA, Van de Winkel JGJ: On the interaction of IgG subclasses with the low affinity FcgammaRlla (CD32) on human monocytes, neutrophils, and platelets; analysis of a functional polymorphism to human IgG2. J Clin Invest 90:1537–1546, 1992

    Google Scholar 

  7. Clark MR, Clarkson SM, Ory PA, Stollman N, Goldstein IM: Molecular basis for a polymorphism involving Fc receptor II on human monocytes. J Immunol 143:1731–1734, 1989

    Google Scholar 

  8. Warmerdam PAM, Van De Winkel JGJ, Gosselin EJ, Capel PJA: Molecular basis for a polymorphism of human Fcgamma receptor II (CD32). J Exp Med 172:19–25, 1990

    Google Scholar 

  9. Warmerdam PAM, van de Winkel JGJ, Vlug A, Westerdaal NAC, Capel PJA: A single amino acid in the second Ig-like domain of the human Fcgamma receptor II is critical for human IgG2 binding. J Immunol 147:1338–1343, 1991

    Google Scholar 

  10. van de Winkel JGJ, Anderson Cl: Biology of human immunoglobulin G Fc receptors. J Leukoc Biol 49:511–524, 1991

    Google Scholar 

  11. van den Herik-Oudijk IE, Westerdaal NAC, Henriquez NV, Capel PJA, van de Winkel JGJ: Functional analysis of human Fcgamma receptor II (CD32) isoforms expressed in B lymphocytes. J Immunol 152:574–585, 1994

    Google Scholar 

  12. Raghavan M, Bjürkman, PJ: Fc receptors and their interactions with immunoglobulins. Annu Rev Cell Dev Biol 12:181–220, 1996

    Google Scholar 

  13. Abo T, Tilden AB, Balch CM, Kumagai K, Troup, GM, Cooper M: Ethnic differences in the lymphocyte proliferative response induced by a murine IgG1 antibody, Leu-4, to the T3 molecule. J Exp Med 160:303–309, 1984

    Google Scholar 

  14. Parren PWHI, Geerts MEJ, Boeije LCM, Aarden LA: Induction of T-cell proliferation by recombinant mouse and chimeric mouse/ human anti-CD3 monocional antibodies. Res Immunol 142:749–763, 1991

    Google Scholar 

  15. Parren PWHI, Warmerdam, PAM, Boeije LCM, Capel PJA, Van de Winkel JGJ, Aarden LA: Characterization of IgG FcR-mediated proliferation of human T-cells induced by mouse and human anti-CD3 monoclonal antibodies; identification of a functinal polymorphism to human IgG2 anti-CD3. J Immunol 148:695–701, 1992

    Google Scholar 

  16. Lahiri DK, Nurnberger JI: A rapid non-enzymatic method for the preparation of HMW DNA from blood for RFLP studies. Nucleic Acids Res 19:5444‐1991

    Google Scholar 

  17. Osborne JM, Chacko GW, Brandt JT, Anderson CL: Ethnic variation in frequency of an allelic polymorphism of human FcgammaRIIA determined with allele specific oligonucleotide probes. J Immunol Methods 173:207–217, 1994

    Google Scholar 

  18. Stuart SG, Trounstine ML, Vaux DJT, Koch T, Martens CL, Mellman I, Moore KW: Isolation and expression of cDNA clones encoding a human receptor for IgG (Fc_RII). J Exp Med 166: 1668–1684, 1987

    Google Scholar 

  19. Brooks DG, Qiu WQ, Luster AD, Ravetch J: Structure and expression of human IgG FcRll (CD32). Functional heterogeneity is encoded by the alternatively spliced products of multiple genes. J Exp Med 170:1369–1385, 1989

    Google Scholar 

  20. Clark MR, Stuart SG, Kimberly RP, Ory PA, Goldstein IM: A single amino acid distingishes the high-responder from the low-responder form of Fc receptor II on human monocytes. Eur J Immunol 21:1911–1916, 1991

    Google Scholar 

  21. Rautonen N, Seppälä I, Hallberg T, Grubb R, Mäkelä O: Determination of homozygosity or heterozygosity for the G2m(n) allotype by a monoclonal, precipitating antibody. Expl Clin Immunogenet6:31–38, 1989

    Google Scholar 

  22. Jefferis R, Reimer CB, Skvaril F, de Lange G, Ling NR, Lowe J, Walker MR, Phillips DJ, Aloisio CH, Wells TW, Vaerman JP, Magnusson CG, Kubagawa H, Cooper M, Vartdal F, Vandvik B, Haaijman JJ, Mäkelä O, Sarnesto A, Lando Z, Gergely J, Rajnavölgyi E, Laszlo G, Radl J, Molinaro GA: Evaluation of monoclonal antibodies having specificity for human IgG subclasses: Results of an IUIS/WHO collaborative study. Immunol Lett 10:223–252, 1985

    Google Scholar 

  23. Joutsi L, Javela K, Partanen J, Kekomäki R: Genetic polymorphism H131R of Fcgamma receptor type IIA (FcgammaRIIA) in a healthy Finnish population and in patients with or without plateletassociated IgG. Eur J Haematol 61:183–189, 1998

    Google Scholar 

  24. Morell A, Skvaril F, Steinberg, AG, van Loghem E, Terry WD: Correlations between the concentrations of the four subclasses of IgG and Gm allotypes in normal human sera. J Immunol 108:195–206, 1972

    Google Scholar 

  25. Bredius RGM, Derkx BHF, Fijen CAP, de Wit TPM, de Haas M, Weening RS, van de Winkel JGJ, Out TA: Fcgamma receptor IIA (CD32) polymorphism in fulminant meningococcal septic shock in children. J Infect Dis 170:848–853, 1994

    Google Scholar 

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Authors and Affiliations

  1. Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Haartmaninkatu 3, FIN-00014, Helsinki, Finland

    Heikki Sarvas, Paula Vesterinen & Olli Mäkelä

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  1. Heikki Sarvas
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  2. Paula Vesterinen
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  3. Olli Mäkelä
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Correspondence to Heikki Sarvas.

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Sarvas, H., Vesterinen, P. & Mäkelä, O. Serum IgG2 Concentration Is Associated with Gm-Allotypes of IgG2 But Not with the R131H Polymorphism of Human Fcγ Receptor Type IIa. J Clin Immunol 22, 92–97 (2002). https://doi.org/10.1023/A:1014431819413

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