Abstract
The differentiating effect of DMSO on K562 cells was studied against the background of pretreatment of the cells by the modulators of activities of protein kinase C and Ca signaling, phorbol 12-myristate 13-acetate, and ionophore A23187. The 2-hour pretreatment of K562 cells with A23187 (1 μM), rather than phorbol 12-myristate 13-acetate (0.1 μM), led to inhibition of the differentiating effect of DMSO (0.1%) during the subsequent four days of incubation. When the cells were pretreated jointly with A23187 and phorbol 12-myristate 13-acetate, the DMSO-induced erythroid differentiation was restored. Analysis of the DNA-degrading effect of the reagents used on K562 cells by fluorescent dyes under the same conditions suggests that this activity is induced in the presence of DMSO in the cells pretreated with a combination of phorbol 12-myristate 13-acetate and A23187 or without pretreatment.
Similar content being viewed by others
REFERENCES
Andersson, L.C., Nilsson, K., and Gahmberg, C.G., K562—a Human Erythroleukemic Cell Line, Int.J.Cancer, 1979, vol. 23, pp. 143–147.
Anisimov, A.G. and Bolotnikov, I.A., Treatment of Synchronized K562 Cells by Tetrafluoroluminate Does Not Modulate Fluorescence of Ethidium Bromide and 4',6-Diamidino-2-Phenylindole upon Binding to Nucleoid DNA, Tsitologiya, 1999, vol. 41, no. 8, pp. 680–684.
Anisimov, A.G., Bolotnikov, I.A., and Volkova, T.O., Influence of Thymidine and Phorbol 12-Myristate 13-Acetate on Erythroid Differentiation of K562 Cells and Their Sensitivity to Unspecific Lysis by Rat Splenocytes, Byull.Eksperim.Biol.Med., 1999, vol. 128, no. 11, pp. 521–524.
Anisimov, A.G., Bolotnikov, I.A., and Volkova, T.O., Changes in Sensitivity of K5652 Cells to Unspecific Lysis by Human and Rat Leucocytes under the Influence of Sodium Butyrate, DMSO, and Phorbol 12-Myristate 13-Acetate, Ontogenez, 2000, vol. 31, no. 1, pp. 47–52.
Arcangeli, A., Ricupero, L., and Olivotto, M., Commitment to Differentiation of Murine Erythroleukemia Cells Involves a Modulated Plasma Membrane Depolarization through Ca2+-Activated K+-channels, J.Cell Physiol., 1987, vol. 132, pp. 387–400.
Baliga, B.S., Mankad, M., Shah, A.K., and Mankad, V.N., Mechanism of Differentiation of Human Erythroleukemic Cell Line K562 by Hemin, Cell Prolif., 1993, vol. 26, pp.519–529.
Butler, T.M., Ziemiecki, A., and Friis, R.R., Megakaryocytic Differentiation of K562 Cells Is Associated with Changes in the Cytoskeletal Organization and the Pattern of Chromatographically Distinct Forms of Phosphotyrosyl-Specific Protein Phosphatases, Cancer Res., 1990, vol. 50, pp. 6323–6329.
Chateau, M.T., Ginestier-Verne, C, Chiesa, J., et al., Dimethyl Sulfoxide-Induced Apoptosis in Human Leukemic U937 Cells, Anal.Cell Pathol., 1996, vol. 10, no. 2, pp. 75–84.
Cook, P.P. and Brazell, L.A., Spectrofluorometric Measurement of the Binding of Ethidium to Superhelical DNA from Cell Nuclei, Eur.J.Biochem., 1978, vol. 84, pp. 465–477.
Crone, T.M., Schalles, S.L., Benedict, C.M., et al., Growth Inhibition by a Triple Ribozyme Targeted to Repetitive B2 Transcripts, Hepatology, 1999, vol. 29, pp. 1114–1123.
Durkin, J.P., Chakravarthy, B., Tremblay, R., et al., Evidence That a Novel Human Differentiation-Inhibiting Protein Blocks the Dimethyl Sulfoxide-Induced Differentiation of Erythroleukemia Cells by Inhibiting the Activation of Membrane Protein Kinase C, Cancer Res., 1992, vol. 52 (22), pp.6329–6334.
Eisbruch. A., Blick, M., Evinger-Hodges, M.J., et al., Effect of Differentiation-Inducing Agents on Oncogene Expression in a Chronic Myelogenous Leukemia Cell Line, Cancer, 1988, vol. 62, pp. 1171–1178.
Farago A. and Nishizuka, Y., Protein Kinase C in Transmembrane Signalling, FEBS Lett., 1990, vol. 268(2), pp. 350–354.
Ginestier-Verne, C., Chateau, M.T., and Bureau, J.P., Implication of Tyrosine Kinases and Protein Kinase C in Dimethyl Sulfoxide-Induced Apoptosis, Anal.Cell.Pathol., 1996, vol. 11(2), pp. 115–126.
Gonchar, M. V., Sergeeva, M.G., Namgaladze, D.A., and Mevkh, A.T., Lack of Direct Connection between Arachidonic Acid Release and Prostanoid Synthesis upon Differentiation of U937 Cell, Biochem.Biophys.Res.Commun., 1998, vol. 249 (3), pp. 829–832.
Grant, S., Turner, A.J., Freemerman, A.J., et al., Modulation of Protein Kinase C Activity and Calcium-Sensitive Isoform Expression in Human Myeloid Leukemia Cells by Bryostatin I: Relationship to Differentiation and Ara-C-Induced Apoptosis, Exp.Cell Res., 1996, vol. 228 (1), pp. 65–75.
Hsu, F.F., Ma, Z., Wohltmann, M., et al., Electrospray Ionization/Mass Spectrometric Analyses of Human Promonocytic U937 Cell Glycerolipids and Evidence that Differentiation Is Associated with Membrane Lipid Composition Changes That Facilitate Phospholipase A2 Activation, J.Biol.Chem., 2000, vol. 275 (22), pp. 16579–16589.
Ivanov, S.D., Postradiation Reactions of DNA of Nucleoids of Blood Leucocytes. Detection, Patterns, and Diagnostic and Prognostic Significance, Doctoral (Biol.) Dissertation, St.-Petersburg, TsNIRRI, 1992.
Ivanov, S.D., Nikolaevskaya, L.V. Fedorov, B.A., and Chukhlovin, A.B., Changes in the Structure of DNA of Nucleoids and Adhesive Properties of Blood Leucocytes at the Early Stages after Irradiation, Byull.Eksperim.Biol.Med., 1992, vol. 114, no. 9, pp. 310–312.
Kimura, H. and Aoyama, T., Decrease in Sensitivity to Ethidium Bromide by Caffeine, Dimethylsulfoxide or 3-Ammobenzamide Due to Reduced Permeability, J.Pharmacobiodyn., 1989, vol. 12, pp. 589–595.
Konstantinova, I.M., Petukhova, O.A., Kulichkova, V.A., et? al., A New Class of Small RNP (α-RNP) Containing Antisense RNA in K562 Cells. 1. Characterization and Changes during Erythroid Differentiation, Ontogenez, 1996, vol. 27, no. 3, pp. 186–192.
Levy, J., Terada, M., Rifkind, R.A., and Marks, P.A., Induction of Erythroid Differentiation by Dimethylsulfoxide in Cells Infected with Friend Virus: Relationship to the Cell Cycle, Proc.Natl.Acad.Sci.USA, 1975, vol. 72, pp. 28–32.
Lin, C.K., Kalunta, C.I., Chen, F.S., et al., Dimethyl Sulfoxide Suppresses Apoptosis in Burkitt's Lymphoma Cells, Exp.Cell Res., 1995, vol. 216, pp. 403–410.
Lukyanov, D.V., Urusova, M.E., Shcherba, K.M., and Podgornaya, O.I., Alu-DNA Repeat-Binding Protein p68 Is a Part of Alu-RNA Containing alpha-RNP, Eur.J.Biochem., 2000, vol. 267, pp. 2362–2371.
Makowske, M., Ballester, R., Cayre, Y., and Rosen, O.M., Immunochemical Evidence that Three Protein Kinase C Isozymes Increase in Abundance during HL-60 Differentiation Induced by Dimethyl Sulfoxide and Retinoic Acid, J.Biol.Chem., 1988, vol. 263 (7), pp. 3402–3410.
Morley, P. and Whitfield, J.F., The Differentiation Inducer, Dimethyl Sulfoxide, Transiently Increases the Intracellular Calcium Ion Concentration in Various Cell Types, J.Cell.Physiol., 1993, vol. 156 (2), pp. 219–225.
Nishizawa, Y., Saeki, K., Hirai, H., et al., Potent Inhibition of Cell Density-Dependent Apoptosis and Enhancement of Survival by Dimethyl Sulfoxide in Human Myeloblastic HL-60 Cells, J.Cell Physiol., 1998, vol. 174, pp. 135–143.
Pincus, S.M., Beckman, B.S., Broadway, R.L., et al., Early Changes in Phosphoprotein Patterns of Friend Erythroleukaemia Cells Induced by Dimethylsulfoxide or Phorbol Esters, Cytobios, 1990, vol. 64 (256), pp. 23–33.
Sutherland, J.A., Turner, A.R., and Mannoni, P., Differentiation of K562 Leukemia Cells along Erythroid, Macrophage, and Megakaryocyte Lineages, J.Biol.Response Mod., 1986, vol. 5, pp.250–262.
Trubiani, O., Rapino, M., Fieri, C., and Di Primio, R., Phorbol Ester Synergizes the Dimethyl Sulfoxide-Dependent Programmed Cell Death through Diacylglycerol Increment, Cancer Detect.Prev., 1998, vol. 22 (5), pp. 463–469.
Tyers, M. and Harley, C.B., Ca2+ and Phorbol Ester Synergistically Induce HL-60 Differentiation, FEBS Lett., 1986, vol. 206 (1), pp. 99–105.
Vrana, J.A., Rao, A.S., Wang, Z., et al., Effects of Bryostatin I and Calcium Ionophore (A23187) on Apoptosis and Differentiation in Human Myeloid Leukemia Cells (HL-60) Following 1-beta-D-Arabinofuranosylcytosine Exposure, Int.J.Oncol., 1998, vol. 12 (4), pp. 927–934.
Wiener, E., Shiels, A., Wickramasinghe, S.N., and Avent, N.D., Effect of 1-beta-D-Arabino-Furanosyl-Cytosine (ara-C) Induction of K562 Cells on Expression of Rh and Other Blood Group Active Proteins, Br.J.Haematol., 1998, vol. 103, pp. 259–267.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Anisimov, A.G., Volkova, T.O., Chekmasova, A.A. et al. Phorbol12-Myristate13-Acetate Relieves the Inhibitory Effect of A23187 on Erythroid Differentiation of K562 Cells Induced by Dimethylsulfoxide. Biology Bulletin 29, 109–114 (2002). https://doi.org/10.1023/A:1014394613810
Issue Date:
DOI: https://doi.org/10.1023/A:1014394613810