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Depolymerization of High-Molecular-Weight Chitosan by the Enzyme Preparation Celloviridine G20x

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Abstract

A low-molecular-weight water-soluble chitosan was obtained from high-molecular-weight crab chitosan using the enzyme preparation Celloviridine G20x. Optimum conditions for enzymatic hydrolysis were designed. The reaction should be performed for 4 h in a sodium-acetate buffer (pH 5.2) at 55°C and an enzyme to substrate ratio of 1 : 400. Fractional extraction of chitosan hydrolysate by aqueous ethanol (ethanol:distilled water) yielded fractions with molecular weights in the range 3.2–26.4 kDa.

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  1. Bioinzheneriya Center of the Russian Academy of Sciences, Moscow, 117312, Russia

    A. V. Il'ina, Yu. V. Tkacheva & V. P. Varlamov

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  1. A. V. Il'ina
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  2. Yu. V. Tkacheva
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  3. V. P. Varlamov
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Il'ina, A.V., Tkacheva, Y.V. & Varlamov, V.P. Depolymerization of High-Molecular-Weight Chitosan by the Enzyme Preparation Celloviridine G20x. Applied Biochemistry and Microbiology 38, 112–115 (2002). https://doi.org/10.1023/A:1014394013017

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