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Differential Scanning Calorimetric and Circular Dichroistic Studies on Plant Antifreeze Proteins

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Abstract

Antifreeze protein (AFP) can lower the freezing point by inhibiting the growth of ice crystals. In this article, the thermal hysteresis activity (THA) of a plant AFP was measured with differential scanning calorimetry (DSC). As is shown, when the amount of ice in the sample was less than 5% THA of this AFP reached as high as 0.35°C. The secondary structure of this AFP was studied with circular dichroism (CD). The CD spectrum from 195to 240 nm indicated a well-defined secondary structure consisting 11% α-helix, 34%antiparallel β-sheet and 55% random coil.

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References

  1. C. L. Hew and D. S. C. Yang, Eur. J. Biochem., 203 (1992) 33.

    Article  CAS  Google Scholar 

  2. J. G. Duman, Biochimica et Biophysica Acta, 1206 (1994) 129.

    CAS  Google Scholar 

  3. W. C. How, M. Griffith, P. Chong and D. S. C. Yang, Plant Physiol, 104 (1994) 971.

    Google Scholar 

  4. T. N. Hansen and J. G. Baust, Biochim. Biophys. Acta, 957 (1988) 217.

    CAS  Google Scholar 

  5. J. G. Duman and T. M. Olsen, Cryobiology, 30 (1993) 322.

    Article  Google Scholar 

  6. V. S. Ananthanarayanan, D. Slaughter and C. L. Hew, Biochim. Biophys. Acta, 870 (1986) 154.

    CAS  Google Scholar 

  7. D. Slaughter, G. L. Fletcher, V. S. Ananthanaryanan and C. L. Hew, J. Bio. Chem., 256 (1981) 2022.

    CAS  Google Scholar 

  8. F. D. Sonnichsen, B. D. Sykes, H. Chao and P. L. Davies, Science, 259 (1993) 1154.

    CAS  Google Scholar 

  9. P. L. Davies and C. L. Hew, FASEB J., 4 (1992) 2460.

    Google Scholar 

  10. A. L. Devries and Y. Lin, Biochim. Biophys. Acta, 495 (1977) 388.

    CAS  Google Scholar 

  11. D. Wen and R. A. Lausen, Biophys. J., 63 (1992) 1659.

    Article  CAS  Google Scholar 

  12. J. G. Duman, J. Comp. Physiol. B, 131 (1979) 347.

    Article  Google Scholar 

  13. J. G. Duman, J. Exp. Zool., 201 (1997) 333.

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Institute of Physical Chemistry, Peking University, Beijing, 100871, P. R. China

    M. Lu, B. Wang & Zh. Li

  2. Institute of Developmental Biology, Chinese Academy of Sciences, Beijing, 100080, P. R. China

    Y. Fei, L. Wei & Sh. Gao

Authors
  1. M. Lu
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  2. B. Wang
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  3. Zh. Li
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  4. Y. Fei
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  5. L. Wei
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  6. Sh. Gao
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Lu, M., Wang, B., Li, Z. et al. Differential Scanning Calorimetric and Circular Dichroistic Studies on Plant Antifreeze Proteins. Journal of Thermal Analysis and Calorimetry 67, 689–698 (2002). https://doi.org/10.1023/A:1014369208229

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  • DOI: https://doi.org/10.1023/A:1014369208229

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