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Anaerobic Degradation of Biphenyl by the Facultative Anaerobic Strain Citrobacter freundii BS2211

Abstract

Using a synthetic medium supplemented with biphenyl (a polycyclic aromatic hydrocarbon), a new bacterial strain of Citrobacter freundiiwas isolated from enrichment cultures containing soil and industrial wastewater samples of the Serpukhov Condenser Factory. This strain was found to be capable of degrading biphenyl under anaerobic conditions in the course of nitrate reduction. When the initial concentration of biphenyl in the culture medium equaled 150 mg/ml, the culture with a titer of 109 cells/ml degraded up to 26–28% of biphenyl in 3 days (28°C). At 250 mg/ml, the culture with a titer of 107 cells/ml degraded 15% of biphenyl in 21 days. Approximately 10% of the substrate consumed was utilized completely, whereas the remainder underwent transformation.

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Grishchenkov, V.G., Slepen'kin, A.V. & Boronin, A.M. Anaerobic Degradation of Biphenyl by the Facultative Anaerobic Strain Citrobacter freundii BS2211. Applied Biochemistry and Microbiology 38, 125–128 (2002). https://doi.org/10.1023/A:1014350230764

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  • DOI: https://doi.org/10.1023/A:1014350230764

Keywords

  • Nitrate
  • Wastewater
  • Hydrocarbon
  • Polycyclic Aromatic Hydrocarbon
  • Bacterial Strain