Abstract
In this study we used invasion and tubulogenesis assays to measure the morphogenetic potential of primary human keratinocytes in vitro. We also used human embryonic and dermal fibroblasts in coculture with keratinocytes and compared their influence on keratinocyte behavior in these different cell culture systems. The keratinocytes retained morphogenetic potential in vitro. In the presence of embryonic fibroblasts, the keratinocytes formed tubes and invaded collagen gel. A three-dimensional architecture of invasion clusters was reconstructed. We conclude that the cellular mechanisms of invasion and tubulogenesis are similar.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Alexandrova, A.Y., Dugina, V.B., Ivanova, O.Y., et al., Scatter Factor Induces Segregation of Multinuclear Cells into Several Discrete Motile Domains, Cell Motil.Cytoskeleton, 1998, vol. 39, pp. 147–158.
Dugina, V.B., Alexandrova, A.Y., Lane, K., et al., The Role of the Microtubular System in the Cell Response to HGF/SF, J.Cell Sci., 1995, vol. 108, pp. 1659–1667.
Asselineau, D., Bernhard, B., Bailly, C., and Darmon, M., Epidermal Morphogenesis and Induction of the 67 kD Keratin Polypeptide by Culture of Human Keratinocytes at the Air-Liquid Interface, Exp.Cell Res., 1985, vol. 159, pp. 536–539.
Brinkmann, V., Foroutan, H., Sachs, M., et al., Hepatocyte Growth Factor/Scatter Factor Induces a Variety of Tissue-Specific Morphogenic Programs in Epithelial Cells, J.Cell Biol., 1995, vol. 131, pp. 1573–1586.
Maas-Szabowski, N., Stark, H.-J., and Fusenig, N.E., Keratinocyte Growth Regulation in Defined Organotypic Cultures through IL-1-Induced Keratinocyte Growth Factor Expression in Resting Fibroblasts, J.Invest.Dermatol., 2000, vol. 114, pp. 1075–1083.
Montesano, R., Matsumoto, K., Nakumura, T., and Orel, L., Identification of a Fibroblast-Derived Epithelial Morphogen as Hepatocyte Growth Factor, Cell, 1991a, vol. 67, pp. 901–908.
Montesano, R., Schaller, G., and Orel, L., Induction of Epithelial Tubular Morphogenesis in vitro by Fibroblast-Derived Soluble Factors, Cell, 1991b, vol. 66, pp. 697–711.
Pentland, A.P., Marcelo, C.L., Jordan, M.A., et al., Effects of Gas Tension on Epidermal Keratinocyte DNA Synthesis and Prostaglandin Production, J.Invest.Dermatol., 1996, vol. 86, pp. 177–180.
Pittelkow, M.R., Cook, P.W., Shipley, G.D., et al., Autonomous Growth of Human Keratinocytes Requires Epidermal Growth Factor Receptor Occupancy, Cell Growth Differ., 1993, vol. 4, pp. 513–521.
Pollack, A.L., Runyan, R.B., and Mostov, K.E., Morphogenetic Mechanisms of Epithelial Tubulogenesis: MDCK Cell Polarity Is Transiently Rearranged without Loss of Cell–Cell Contact during Scatter Factor/Hepatocyte Growth Factor-Induced Tubulogenesis, Dev.Biol., 1998, vol. 204, pp. 64–79.
Saitoh, K., Takahashi, H., Sawada, N., and Parsons, P.G., Detection of the c-met Proto-Oncogene Product in Normal Skin and Tumors of Melanocytic Origin, J.Pathol., 1994, vol. 174, pp. 191–199.
Sato, C., Tsuboi, R., Shi, C.M., et al., Comparative Study of Hepatocyte Growth Factor/Scatter Factor and Keratinocyte Growth Factor Effects on Human Keratinocytes, J.Invest.Dermatol., 1995, vol. 104, pp. 958–963.
Smola, H., Thiekotter, G., and Fusenig, N.E., Mutual Induction of Growth Factor Gene Expression by Epidermal–Dermal Cell Interaction, J.Cell Biol., 1993, vol. 122, pp. 417–429.
Stoker, M., Gherardi, E., Ferryman, M., and Gray, J., Scatter Factor Is a Fibroblast-Derived Modulator of Epithelial Cell Mobility, Nature, 1987, vol. 327, pp. 239–242.
Trusolino, L., Cavassa, S., Angelini, P., et al., HGF/Scatter Factor Selectively Promotes Cell Invasion by Increasing Integrin Avidity, FASEB J., 2000, vol. 14, pp. 1629–1640.
Turken, K., Choi, Y., and Fuchs E., Transforming Growth Factor α Induces Collagen Degradation and Cell Migration in Differentiating Human Epidermal Raft Cultures, Cell Reg., 1991, vol. 2, pp. 613–625.
Vasiliev, A.,V., Voloshin, A.V., Vorotelyak, E.A., and Terskikh, V.V., Migration of the Colonies of Epidermal Keratinocytes in Culture, Dokl.Ross.Akad.Nauk, 1993, vol. 329, pp. 232–235.
Vorotelyak, E.A., Migration of Epidermal Human Cells in Culture, Cand. Sci. (Biol.) Dissertation, Moscow, 1998.
Vorotelyak, E.A., Leonova, O.G., Shinin, V.V., et al., Growth of Human Epidermal Keratinocytes on Collagen Gel, Dokl.Ross.Akad.Nauk, 1999, vol. 369, no. 5, pp. 232–235.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shinin, V.V., Chernaya, O.G. & Terskikh, V.V. Induction of Invasion and Tubulogenesis in Primary Human Keratinocyte Culture. Biology Bulletin 29, 101–108 (2002). https://doi.org/10.1023/A:1014347629739
Issue Date:
DOI: https://doi.org/10.1023/A:1014347629739