Abstract
Formation kinetics, specificity, and analytical potential of polyclonal antibodies raised in rabbits against BSA conjugates of zearalanone carboxymethyloxime (CMO-ZAN) and zearalenone carboxymethyloxime (CMO-ZEN) have been studied. Preparation of the conjugates involved conversion of CMO-ZAN and CMO-ZEN into activated esters or carbodiimide condensation. Two versions of a group-specific enzyme immunoassay (for zearalenone/α-zearalenol and zearalanone/α-zearalanol) based on the heterologous combination of solid-phase antigens are described (sensitivity, 0.01 ng/ml).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Potter, S.M., Baum, J.A., Teng, H., et al., Am. J. Clin. Nutr., 1998, vol. 68, no. 9, pp. 1375–1379.
Wang, G., Kuan, S.S., Francis, O.J., et al., J. Agric. Food. Chem., 1990, vol. 38, no. 2, pp. 185–190.
Gestetner, B., Ishaaya, I., Birk, Y., and Bondi, A., Isr. J. Chem., 1963, vol. 1, no. 3, pp. 460–466.
Tekhnologiya pererabotki zhirov (Technology of Lipids Processing), Arutyunyan, N.S., Ed., Moscow: Nauka, 1975.
Martynova, O.M., Sorokoumova, G.M., Selishcheva, A.A., et al., Byull. Eksp. Biol. Med., 2000, vol. 129, pp. 159–162.
Svetashev, V.I. and Vaskovsky, V.E., J. Chromatogr., 1972, vol. 65, no. 4, pp. 451–455.
Kates, M., Techniques of Lipidology, Amsterdam: Elsevier, 1972.
Kirchner, J., Thin-Layer Chromatography, New York: Wiley, 1978, vol. 1.
Ditmer, J.C. and Lester, R.L., J. Lipids, 1964, vol. 5, no. 1, pp. 126–129.
Bardygula-Nonn, L.G., Raster, J.I., and Glonek, T., Lip-ids, 1995, vol. 30, no. 7, pp. 1047–1051.
Rodriguer-Vico, F., Caynela, M., Perigrin, E., and Ramirez, H., Anal. Biochem., 1989, vol. 183, no. 2, pp. 275–278.
Birk, Y., Bondi, A., Gestetner, B., and Ishaaya, I., Nature (London), 1963, vol. 197, no. 4872, pp. 1089–1090.
Ivanova, S.D., Dubil'nye veshchestva, saponiny, antratsen-proizvodnye i flavonoidy v lekarstvennykh preparatakh (Tannins, Saponins, Anthracene Products, and Flavonoids in Medicinal Preparations), Moscow: Nauka, 1961.
Gestetner, B., Birk, Y., Bondi, A., and Teneer, Y., Phy-tochemistry, 1966, vol. 5, no. 5, pp. 803–806.
Vladimirov, Yu.A., Fotokhimiya i lyuministsentsiya belkov (Photochemistry and Protein Luminescence), Moscow: Mir, 1965.
Wolf, W.J. and Thomas, B.W., J. Chromatogr., 1971, vol. 56, no. 2, pp. 281–293.
Shiraiwa, M., Kudo, S., Shimoyamada, M., et al., Agric. Biol. Chem., 1991, vol. 55, no. 2, pp. 315–322.
Kersten, G., Spiekstra, A., Beuvery, E., and Crommelin, D., Biochim. Biophys. Acta, 1991, vol. 1062, no. 2, pp. 165–171.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Burkin, A.A., Kononenko, G.P. & Soboleva, N.A. Group-Specific Antibodies against Zearalenone and Its Metabolites and Synthetic Analogs. Applied Biochemistry and Microbiology 38, 169–176 (2002). https://doi.org/10.1023/A:1014318818469
Issue Date:
DOI: https://doi.org/10.1023/A:1014318818469