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Inhibition of IAA-Induced Growth of Wheat Coleoptile Fragments by Chitin-Chitosan Oligomers

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Abstract

We studied the comparative effects of the phytohormone indolylacetic acid (IAA) and chitooligosaccharides (5–10 kDa, degree of deacetylation 35%) on the growth of coleoptile fragments of 3-day wheat germlings. IAA (10mg/l) stimulated elongation of coleoptile fragments by 80%, as compared to the control (water). Chitooligosaccharides at 0.01–1500 mg/l or higher did not exert auxin-like effects, but at 100 mg/l or higher, suppressed elongation of the coleoptile fragments, as compared to the control (water). Incubation of coleoptile fragments in solutions containing both IAA and chitooligosaccharides (100 mg/l or higher) suppressed their IAA-induced elongation, which correlated with the inhibition of growth of 3-day wheat germlings after wetting seeds in solutions of chitooligosaccharides. It has been proposed that the phytohormone-like properties of chitooligosaccharides can be related to changes in the endogenous balance of phytohormones in plants.

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Authors and Affiliations

  1. Department of Biochemistry and Cytochemistry, Ufa Scientific Center of the Russian Academy of Sciences, pr. Oktyabrya 69, Ufa, 450054, Russia

    R. M. Khairullin, I. E. Akhmetova & Z. R. Yusupova

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  1. R. M. Khairullin
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  2. I. E. Akhmetova
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  3. Z. R. Yusupova
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Khairullin, R.M., Akhmetova, I.E. & Yusupova, Z.R. Inhibition of IAA-Induced Growth of Wheat Coleoptile Fragments by Chitin-Chitosan Oligomers. Biology Bulletin 29, 135–138 (2002). https://doi.org/10.1023/A:1014302915627

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