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Presynaptic Modulation of K+-Evoked [3H]Dopamine Release in Striatal and Frontal Cortical Synaptosomes of Normotensive and Spontaneous-Hypertensive Rats

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Abstract

Regional differences in presynaptic [3H]dopamine ([3H]DA) release and its modulation by D2 DA-receptors between the frontal cortex and striatum obtained from Wystar-Kyoto (WKY) and spontaneous-hypertensive rats (SHR) have been evaluated using superfused synaptosomes. Synaptosomal tritium content was significantly lower in the frontal cortex than in the striatum in both SHR and WKY (∼45% and 48%, respectively), but no differences in tritium content were obtained between strains. However, the 15 mM K+-evoked [3H]DA overflow was lower in the SHR as compared to WKY rats in both brain regions (striatum ∼23%, frontal cortex ∼21). Concentration-response curves for quinpirole (1nM-10 μM)-mediated inhibition of 15mM K+-evoked [3H]DA release showed no differences between SHR and WKY. These results suggest that SHR has less ability to release [3H]DA as compared to WKY rats, but SHR did not show differences in the autoregulation of such release in both the frontal cortex and striatum.

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Authors and Affiliations

  1. Departament de Farmacologia i Terapèutica, Facultat de Medicina, Univeristat Autònoma de Barcelona, Spain

    Moulay A. Yousfi-Alaoui, Susanna Hospital, Alicia Garcia-Sanz, Albert Badia & M. Victoria Clos

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  1. Moulay A. Yousfi-Alaoui
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  2. Susanna Hospital
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  3. Alicia Garcia-Sanz
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  4. Albert Badia
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  5. M. Victoria Clos
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Yousfi-Alaoui, M.A., Hospital, S., Garcia-Sanz, A. et al. Presynaptic Modulation of K+-Evoked [3H]Dopamine Release in Striatal and Frontal Cortical Synaptosomes of Normotensive and Spontaneous-Hypertensive Rats. Neurochem Res 26, 1271–1275 (2001). https://doi.org/10.1023/A:1014237215200

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