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Synthesis of peptide-modified pyoverdins by a fluorescent Pseudomonas strain grown in isoleucine-supplemented medium

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Abstract

The pattern of pyoverdin-type siderophoresproduced by Pseudomonas putida BTP16 in iron-limitedsuccinate medium supplemented or not with isoleucine wascharacterized by electrospray ionization mass spectrometry.Two new compounds were observed in culture supernatantsfrom BTP16 grown in the presence of isoleucine. For bothmolecules, mass differences with pyoverdins originallysynthesized by the strain were only interpreted consideringthe incorporation of Ile into the peptide chain.Further evidence supporting this phenomenon was provided byradioactivity incorporation in pyoverdins produced in thepresence of 14C-labeled Ile. Our results are discussedin relation to the non-ribosomal mechanism supposedto be involved in the synthesis of these molecules.

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Author notes

  1. Marc Ongena

    Present address: Centre Wallon de Biologie Industrielle, Université de Liège, Bâtiment 40, B-4000, Liège/Sart Tilman, Belgium

Authors and Affiliations

  1. Centre Wallon de Biologie Industrielle, Unité de Bioindustries, Faculté Universitaire des Sciences Agronomiques, B-5030, Gembloux, Belgium

    Marc Ongena

  2. Centre Wallon de Biologie Industrielle, Unité de Bioindustries, Faculté Universitaire des Sciences Agronomiques, B-5030 Gembloux, Belgium

    Philippe Jacques & Philippe Thonart

  3. Laboratoire de Spectrométrie de Masse, Université de Liège, Bâtiment B6, B-4000, Liège/Sart-Tilman

    Edwin de Pauw

Authors
  1. Marc Ongena
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  2. Philippe Jacques
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  3. Edwin de Pauw
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  4. Philippe Thonart
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Correspondence to Marc Ongena.

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Ongena, M., Jacques, P., de Pauw, E. et al. Synthesis of peptide-modified pyoverdins by a fluorescent Pseudomonas strain grown in isoleucine-supplemented medium. Letters in Peptide Science 8, 21–27 (2001). https://doi.org/10.1023/A:1014225628123

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