Skip to main content
Log in

Preservation methods for the storage of anaerobic sludges

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Different preservation methods were evaluated for the storage of anaerobic sludges: room temperature, refrigeration at 4 °C, freezing at −20 °C and freeze-drying. Specific methanogenic activity for glucose and acetate were used as indicators of the subsequent recovery of the anaerobic sludge. Storage at room temperature and refrigeration resulted in a better conservation of the methanogenic activity than freezing and freeze-drying.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bae BU, Shin HS, Paik BC, Chung JC (1995) Re-activation characteristics of preserved anaerobic granular sludges. Bioresour. Technol. 53: 231-235.

    Google Scholar 

  • Coates JD, Coughlan MF, Colleran E (1996) Simple method for the measurement of the hydrogenotrophic methanogenic activity of anaerobic sludges. J. Microbiol. Meth. 26: 237-246.

    Google Scholar 

  • Colleran E, Concannon F, Golden T, Geoghegan F, Crumlish B, Killilea E, Henry M, Coates J (1992) Use of methanogenic activity tests to characterize anaerobic sludges, screen for anaerobic biodegradability and determine toxicity thresholds against individual anaerobic trophic groups and species. Water Sci. Tech. 25: 31-40.

    Google Scholar 

  • Hippe H (1984) Maintenance of methanogenic bacteria. In: Kirsop BE, Snell JJS, eds. Maintenance of Microorganisms. A Manual of Laboratory Methods. London: Academic Press, pp. 69-82.

    Google Scholar 

  • Impey CS, Phillips BA (1984) Maintenance of anaerobic bacteria. In: Kirsop BE, Snell JJS, eds. Maintenance of Microorganisms. A Manual of Laboratory Methods. London: Academic Press, pp. 47-56.

    Google Scholar 

  • Shin HS, Bae BU, Oh SE (1993) Preservation characteristics of anaerobic granular sludge. Biotechnol. Lett. 15: 537-542.

    Google Scholar 

  • Soubes M, Muxi L, Fernadez A, Tarlera S, Queirolo M (1994) Inhibition of methanogenesis from acetate by Cr+3 and ammonia. Biotechnol. Lett. 16: 195-200.

    Google Scholar 

  • Soubes M, Muxi L, Zunino L, Fernandez A (1989) Microbiological degradation of lactate under methanogenic conditions. MIRCEN J. Appl. Microbiol. Biotechnol. 5: 193-198.

    Google Scholar 

  • Touzel JP, Albagnac G (1983) Isolation and characterization of Methanococcus mazei strain MC3. FEMS Microbiol. Lett. 16: 241-245.

    Google Scholar 

  • Wu WM, Jain MK, Thiele JH, Zeikus JG (1995) Effect of storage on the performance of methanogenic granules. Water Res. 29: 1445-1452.

    Google Scholar 

  • Yukselen MA (1997) Preservation characteristics of UASB sludges J. Environ. Sci. Hlth. A32: 2069-2076.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Castro, H., Queirolo, M., Quevedo, M. et al. Preservation methods for the storage of anaerobic sludges. Biotechnology Letters 24, 329–333 (2002). https://doi.org/10.1023/A:1014080526608

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1014080526608

Navigation