Abstract
A microinjection process is described that involves the photodynamically-assisted puncture of an animal cell membrane by means of a brief and partial degeneration resulting from cell membrane damage. The ratio of dye-injected rat pheochromocytoma cells that became detached three and six days after photodynamically-assisted microinjection was 9%; however, it was 83% when a conventional mechanical microinjection process was used.
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Saito, T.K., Muguruma, H. & Mabuchi, K. Photodynamic assistance increases the efficiency of the process of microinjection in animal cells. Biotechnology Letters 24, 309–314 (2002). https://doi.org/10.1023/A:1014072904009
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DOI: https://doi.org/10.1023/A:1014072904009