Abstract
Gonadotropin-releasing hormone (GnRH) molecular variants were characterized by gradient reverse phase high performance liquid chromatography (RP)-HPLC) from brain extracts of two perciforms with economic importance for Argentina and Uruguay. RP-HPLC fractions were tested in radioimmunoassays (RIAs) with both poly-specific and specific antisera. Both species showed the presence of the same three molecular forms, immunologically and chromatographically indistinguishable from sbGnRH, cGnRH-II and sGnRH. This study supports the hypothesis that their expression is a common pattern in perciforms.
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Montaner, A., Miranda, L., Vizziano, D. et al. Gonadotropin-releasing hormone in two perciform fishes: Micropogonias furnieri and Pagrus pagrus . Fish Physiology and Biochemistry 24, 243–246 (2001). https://doi.org/10.1023/A:1014062415118
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DOI: https://doi.org/10.1023/A:1014062415118
- GnRH
- perciform fish
- radioimmunoassay