Abstract
Samphire Hoe is a newly-created land platform comprising the sub-seabed material excavated during the construction of the Channel tunnel. It represents a unique resource where the arrival and establishment of arbuscular mycorrhizal fungi (AMF) within a sown plant community on a low nutrient substrate can be monitored. Arbuscular mycorrhizal fungi invasion was monitored in a number of ways: by assessing the degree of root colonisation within the roots of plants on the site, by using a successive trap culture technique to determine AMF species richness, and by using sterile substrate bins to determine the extent of wind-borne and rain-dispersed immigration of AMF propagules into the site. Levels of colonisation of indigenous plants by AMF were high in May–June (the pre-flowering phase of growth for many plants) reflecting the important role of the mycorrhizal symbiosis in dry, low nutrient soils. Twelve species of AMF were identified, representing a relatively high diversity for a recently deposited subsoil. An on-site experiment indicated that inoculum of AMF could enter the site within 8 months and that wind dispersal and/or rain were possible vectors. A field experiment compared the outplanting performance of commercially-produced Elymus pycnanthus seedlings (in a commercial compost with added nutrients) with seedlings produced in a low nutrient substrate and inoculated with AMF isolated from the site (a mixture of 5 species of Glomus) or left uninoculated. After 14 months in the field seedlings, inoculated with the indigenous AMF, had the same tiller production as commercially-produced plants, despite slower initial growth. In contrast, non-mycorrhizal controls grew very poorly with a greater frequency of plant mortality compared with the other treatments. Elymus seedlings inoculated with the indigenous AMF ultimately produced approximately seven times the mean number of seed spikes per surviving plant as commercially-produced seedlings and five times greater weight of seed spike. A phyto-microbial approach to the revegetation of nutrient-poor soils is proposed to stimulate plant successional processes as a economically-viable sustainable input for landscaping anthropogenic sites.
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References
Allen MF (1987) Re-establishment of mycorrhizas on Mount St Helens: migration vectors. Trans. Brit. Mycol. Soc. 88: 413–417
Allen MF, Hipps LE and Wooldridge GL (1989) Wind dispersal and subsequent establishment of VA mycorrhizal fungi across a successional arid landscape. Landscape Ecol 2: 165–171
Brundrett M (1991) Mycorrhizas in natural ecosystems. Adv. Ecol. Res. 21: 171–313
Brundrett MC, Abbott LK and Jasper DA (1999) Glomalean mycorrhizal fungi from tropical Australia. 1. Comparison of the effectiveness and specificity of different isolation procedures. Mycorrhiza 8: 305–314
Carey PD, Fitter AH and Watkinson AR (1982) A field study using the fungicide benomyl to investigate the effect of mycorrhizal fungi on plant fitness. Oecologia 90: 550-555
DeMars BG and Boerner REJ (1996) Vesicular arbuscular mycorrhizal development in the Brassicaceae in relation to plant life span. Flora 191: 179–189
Dodd JC, Boddington CL, Rodriguez A, Gonzalez-Chavez C and Mansur I (2000) Mycelium of Arbuscular Mycorrhizal fungi (AMF) from different genera: form, function and detection. Plant and Soil 226: 131–151
Gould AB and Hendrix JW (1998) Relationship of mycorrhizal activity to time following reclamation of surface mine land in western Kentucky.II. Mycorrhizal fungal communities. Can. J. Bot. 76: 204–212
Harley JL and Harley EL (1987) A check list of mycorrhiza in the British Flora. New Phytol. (Suppl) 105:1–102
Kershaw KR, Mitchley J, Buckley GP and Helliwell DR (1995) Slope protection and establishment of vegetation on Channel Tunnel spoil in an environmentally sensitive coastal site. In: Barker DH (ed) Vegetation and Slopes, pp 117–126. Institute of Civil Engineering. Thomas Telford, London
Koide RT and Lu X (1992) Mycorrhizal infection of wild oats: maternal effects on offspring growth and reproduction. Oecologia 90: 218–226
Koide RT (2000) Mycorrhizal symbiosis and plant reproduction. In: Kapulnik Y and Douds D (eds) Arbuscular Mycorrhizas: Physiology and Function, pp 19–46. Kluwer Academic Publishers, Dordrecht, The Netherlands
Koske RE (1987) Distribution of VA mycorrhizal fungi along a latitudinal temperature gradient. Mycologia 79: 55–68
Lee PJ and Koske RE (1994) Gigaspora gigantea: parasitism of spores by fungi and actinomycetes.Mycol. Res. 98: 458–466
Lu X and Koide RT (1991) Avena fatua L. seed and seedling nutrient dynamics as influenced by mycorrhizal infection of the maternal generation. Plant Cell Environ. 14: 931–939
McMillen BG, Juniper S and Abbott LK (1998) Inhibition of hyphal growth of a vesicular arbuscular mycorrhizal fungus in soil containing sodium chloride limits the spread of infection from spores. Soil Biol. Biochem. 30: 1639–1646
Merryweather JW and Fitter A (1998) The arbuscular mycorrhizal fungi of Hyacinthoides non-scripta. I. Diversity of fungal taxa. New Phytol. 138: 117–129
Mitchley J and Buckley P (1995) Habitat creation on Channel Tunnel Spoil in a maritime environment at Dover, UK. Land Contamination and Reclamation 3: 150–152
Mitchley J, Buckley GP and Helliwell DR (1996) Vegetation establishment on chalk marl spoil: the role of nurse grass species and fertiliser application. J. Vegetation Sci. 7: 543–548
Pfleger FL, Stewart EL and Noyd RK (1994) Role of VAM fungi in mind land revegetation In: Pfleger FL and Linderman RG (eds)Mycorrhizae and Plant Health, pp 47–81. American Phytopathological Society Press, St. Paul, Minnesota
Phillips JM and Hayman DS (1970) Improved procedure for clearing roots and staining parasitic and vesicular arbuscular fungi for rapid assessment of infection. Trans. Brit. Mycol. Soc. 55: 158–161
Stutz JC and Morton JB (1996) Successive pot cultures reveal high species richness of arbuscular endomycorrhizal fungi in arid ecosystems. Can. J. Bot. 74: 1883–1889
Tsang A and Maun MA (1999) Mycorrhizal fungi increase salt tolerance of Strophostyles helvola in coastal foredunes. Plant Ecol. 144: 159–166
Van der Heijden MGA, Boller T, Wiemken A and Sanders IR (1998a) Different arbuscular mycorrhizal fungal species are potential determinants of plant community structure. Ecology 79: 2082–2091
Van der Heijden MGA, Klironimos JN, Ursic M, Moutoglis P, Streitwolf-Engel R, Boller T, Wiemken A and Sanders IR (1998b) Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nature 396: 69–72
Warner NJ, Allen MF and MacMahon JA (1987) Dispersal agents of vesicular-arbuscular mycorrhizal fungi in a disturbed arid ecosystem. Mycologia 79: 721–730
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Dodd, J.C., Dougall, T.A., Clapp, J.P. et al. The role of arbuscular mycorrhizal fungi in plant community establishment at Samphire Hoe, Kent, UK – the reclamation platform created during the building of the Channel tunnel between France and UK. Biodiversity and Conservation 11, 39–58 (2002). https://doi.org/10.1023/A:1014062311463
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DOI: https://doi.org/10.1023/A:1014062311463