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Polymerase-chain-reaction-based detection of individual polyhydroxyalkanoate synthase phaC1 and phaC2 genes*,**

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Abstract

A semi-nested PCR method for the specific and individual amplification of type II phaC1 and phaC2 subgenomic fragments containing the catalytically important lipase box-like sequence and the phosphopantetheine binding site was developed. Direct sequencing of these ΔphaC1 and ΔphaC2 fragments is possible, thus greatly facilitating the characterization of these genes. The method was used to show that three strains of Pseudomonas oleovorans harbor different pha loci, while five strains of P. corrugata contain identical ΔphaC1 and ΔphaC2 subgenomic fragments.

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Authors and Affiliations

  1. ARS, Eastern Regional Research Center, U.S. Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA

    Daniel K.Y. Solaiman

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  1. Daniel K.Y. Solaiman
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Solaiman, D.K. Polymerase-chain-reaction-based detection of individual polyhydroxyalkanoate synthase phaC1 and phaC2 genes*,** . Biotechnology Letters 24, 245–250 (2002). https://doi.org/10.1023/A:1014017609866

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  • DOI: https://doi.org/10.1023/A:1014017609866

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