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Bovine serum albumin-bovine hemoglobin conjugate as a candidate blood substitute

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Abstract

A bovine serum albumin-bovine hemoglobin conjugate was prepared using 3-maleimidobenzoic acid N-hydroxysuccinimide ester as cross-linker. The conjugate was purified using DEAE Sepharose. It had an M r of 127 kDa. Its P50 (half-saturated O2 pressure) value and Hill coefficient were 27 mm Hg and 2, respectively.

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Authors and Affiliations

  1. National Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100080, P.O. Box 353, P.R. China

    Tao Hu & Zhiguo Su

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  1. Tao Hu
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  2. Zhiguo Su
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Hu, T., Su, Z. Bovine serum albumin-bovine hemoglobin conjugate as a candidate blood substitute. Biotechnology Letters 24, 275–278 (2002). https://doi.org/10.1023/A:1014005924482

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  • DOI: https://doi.org/10.1023/A:1014005924482

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